Point Deletion or Insertion in CmeR-Box, A2075G Substitution in 23S rRNA, and Presence of erm(B) Are Key Factors of Erythromycin Resistance in Campylobacter jejuni and Campylobacter coli Isolated From Central China

Cheng, Ye; Zhang, Wenting; Lu, Qin; Wen, Guoyuan; Zhao, Zijin; Luo, Qing; Shao, Hongxia; Zhāng, Téngfēi

doi:10.3389/fmicb.2020.00203

Cited by 7 publications

(2 citation statements)

References 38 publications

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“…The latter has also been found in several other studies [ 54 ]. Two point mutations in 23S (A2075G) and gyrA (T86I) genes associated with erythromycin and quinolone resistance [ 52 , 55 ] in campylobacters were also found in our study. In addition, a previous study conducted in Estonia found gyrA (T86I) mutation in quinolone-resistant C. jejuni ST5 isolates [ 56 ].…”

Section: Discussionsupporting

confidence: 75%

Antibiotic Resistance in Campylobacter spp. Isolated from Broiler Chicken Meat and Human Patients in Estonia

et al. 2022

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Poultry meat is considered the most important source of Campylobacter spp. Because of rising antimicrobial resistance in Campylobacter spp., this study investigated the antimicrobial resistance of Campylobacter isolates from fresh broiler chicken meat originating from the Baltic countries sold in Estonian retail settings. Additionally, human clinical isolates obtained from patients with Campylobacter enteritis in Estonia were analysed. The aim of this study was to investigate the susceptibility of Campylobacter spp. to nalidixic acid, ciprofloxacin, tetracycline, streptomycin, erythromycin and gentamicin. The broth microdilution method with the EUCAMP2 panel was used for MIC determination and antimicrobial mechanisms were analysed using WGS data. A total of 46 Campylobacter strains were analysed, of which 26 (42.6%) originated from Lithuanian, 16 (26.2%) from Latvian, and 4 (6.6%) from Estonian fresh broiler chicken meat. In addition, 15 (24.6%) Campylobacter strains of patients with campylobacteriosis were tested. The antimicrobial resistance patterns of Campylobacter spp. isolated from fresh broiler chicken meat samples of Estonian, Latvian and Lithuanian origin collected in Estonian retail, and from patients with Campylobacter enteric infections, were determined. A total of 46 (75%) of the isolates tested were C. jejuni and 15 (25%) were C. coli. Campylobacter resistance was highest to nalidixic acid (90.2% of strains) and ciprofloxacin (90.2%), followed by tetracycline (57.4%), streptomycin (42.6%) and erythromycin (6.6%). All strains were sensitive to gentamicin. Additionally, antimicrobial resistance genes and point mutations were detected in 27 C. jejuni and 8 C. coli isolates previously assigned as resistant with the phenotypic method. A high antibiotic resistance of Campylobacter spp. in Lithuanian- and Latvian-origin broiler chicken meat and Estonian clinical isolates was found. Similar antibiotic resistance patterns were found for broiler chicken meat and human Campylobacter isolates.

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Section: Discussionsupporting

confidence: 75%

Antibiotic Resistance in Campylobacter spp. Isolated from Broiler Chicken Meat and Human Patients in Estonia

et al. 2022

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“…Para produção Research, Society and Development, v. 9, n. 7, e356974326, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i7.4326 6 de energia, há a dependência do ciclo do ácido cítrico, o que possibilita a degradação de aminoácidos. Essa bactéria é incapaz de produzir energia por meio de carboidratos, sendo o ferro, imprescindível para seu crescimento (Melo, 2017;Cheng et al, 2020).…”

Section: Fisiopatologia Da Gastroenterite Causada Por C Jejuniunclassified

Fatores associados ao impacto de gastroenterites por Campylobacter jejuni

Lima¹,

Oliveira

2020

RSD

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Campylobacter jejuni is a bacteria that is involved in the causality of several pathologies, from asymptomatic cases and others with great amplitude to the propensity of death. Its form of acquisition is facilitated, and the human being can become infected by the consumption of contaminated water and food, prepared incorrectly or under poor hygiene techniques. Because it has the possibility of adhesion and synthesis of toxins, there is a large-scale increase in its potential for pathogenicity. In Brazil, this microorganism is detected in cases of gastroenteritis in children. In developed countries it is the main cause of traveler's diarrhea. This work addresses the morphological characteristics of Campylobacter jejuni, its virulence and epidemiology factor, in addition to the possible diseases caused. To carry out the research, a literature review was carried out, based on publications indexed in the databases: Scielo Google Scholar and Pubmed, published between 2010 and 2020. The descriptors used were: Campylobacter Infections; Gastroenteritis; Campylobacter jejuni, where there were 731 articles, with only 44 selected. The Polymerase Chain Reaction, for measuring the genes of the CDT, is a differential for infectious, always combined with other laboratory and clinical data for a conclusive diagnosis. The use of antimicrobials for the treatment, prevention and / or growth of animals for slaughter has led to an increase in cases of resistance related to microbiota, including C. jejuni.

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Natural Competence and Horizontal Gene Transfer in Campylobacter

Golz

Stingl

2021

Current Topics in Microbiology and Immunology

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Thermophilic Campylobacter, in particular Campylobacter jejuni, C. coli and C. lari are the main relevant Campylobacter species for human infections. Due to their high capacity of genetic exchange by horizontal gene transfer (HGT), rapid adaptation to changing environmental and host conditions contribute to successful spreading and persistence of these foodborne pathogens. However, extensive HGT can exert dangerous side effects for the bacterium, such as the incorporation of gene fragments leading to disturbed gene functions. Here we discuss mechanisms of HGT, notably natural transformation, conjugation and bacteriophage transduction and limiting regulatory strategies of gene transfer. In particular, we summarize the current knowledge on how the DNA macromolecule is exchanged between single cells. Mechanisms to stimulate and to limit HGT obviously coevolved and maintained an optimal balance. Chromosomal rearrangements and incorporation of harmful mutations are risk factors for survival and can result in drastic loss of fitness. In Campylobacter, the restricted recognition and preferential uptake of free DNA from relatives are mediated by a short methylated DNA pattern and not by a classical DNA uptake sequence as found in other bacteria. A class two CRISPR-Cas system is present but also other DNases and restriction–modification systems appear to be important for Campylobacter genome integrity. Several lytic and integrated bacteriophages have been identified, which contribute to genome diversity. Furthermore, we focus on the impact of gene transfer on the spread of antibiotic resistance genes (resistome) and persistence factors. We discuss remaining open questions in the HGT field, supposed to be answered in the future by current technologies like whole-genome sequencing and single-cell approaches.

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Point Deletion or Insertion in CmeR-Box, A2075G Substitution in 23S rRNA, and Presence of erm(B) Are Key Factors of Erythromycin Resistance in Campylobacter jejuni and Campylobacter coli Isolated From Central China

Cited by 7 publications

References 38 publications

Antibiotic Resistance in Campylobacter spp. Isolated from Broiler Chicken Meat and Human Patients in Estonia

Antibiotic Resistance in Campylobacter spp. Isolated from Broiler Chicken Meat and Human Patients in Estonia

Fatores associados ao impacto de gastroenterites por Campylobacter jejuni

Natural Competence and Horizontal Gene Transfer in Campylobacter

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