Genotypic differences in organogenesis from callus of ten triticale lines

Sharma, Govind C.; Bello, L. L.; Sapra, V. T.

doi:10.1007/bf00023222

Cited by 28 publications

(10 citation statements)

References 7 publications

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“…There was no correlation between a line's ability to produce callus and the ability of that callus to regenerate a plant (Rakoczy-Trojanovska and Melepszy, 1993). Genotype has also been found to affect both callus formation and plant regeneration from immature embryos in the other cereal species (reviewed by Bhaskaran and Smith, 1990), in particular the economically important cereals such as wheat (Sears and Deckard, 1982;Machii et al, 1998), barley (Hanzel et al, 1985;Castillo et al, 1998;Baillie et al, 1993b), oats (Gana et al, 1995) and triticale (Sharma et al, 1980).…”

Section: Introductionmentioning

confidence: 93%

“…Immature embryos are the most widely reported explant, having been used successfully to regenerate rye (Eapen and Rao, 1982;Lu et al, 1984;Castillo et al, 1994), triticale (Sharma et al, 1980;Eapen and Rao, 1982), wheat (Ozias-Akins and Vasil, 1982;Fennell et al, 1996;Machii et al, 1998), barley (Hanzel et al, 1985;Goldstein and Kronstad, 1986;Bregitzer et al, 1998) and oats (Gana et al, 1995). The use of immature embryos as the explant is labor intensive as plants must be maintained and grown almost to maturity to obtain the young embryos.…”

Section: Introductionmentioning

confidence: 98%

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Callus formation and plant regeneration from immature and mature embryos of rye (Secale cereale L.)

Ward

Jordan

2001

In Vitro Cell.Dev.Biol.-Plant

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An efficient protocol was developed to regenerate entire plants from immature embryos of elite genotypes of rye as a prerequisite to plant transformation. Three winter genotypes and one spring genotype were tested using both immature and mature embryos as explants. Four types of callus initiation media and five kinds of regeneration media were tested in all possible combinations. Immature embryos gave much higher levels of plant regeneration than mature embryos, but mature embryos could be induced to regenerate plants for all genotypes and media tested, although at low levels. A minimum stage of embryo development must be reached before embryos can be cultured successfully. Genotypic effects were less pronounced than those reported for inbred cereal species such as wheat and barley, but there was an effect of genotype on percentage of callus formation. There was a significant interaction between genotype and initiation media. Composition of the initiation media affected both the percentage of callus formation from embryos and subsequent frequencies of plant regeneration. Composition of the regeneration media had no effect on level of plant regeneration. Immature embryos of all genotypes tested could be induced to produce 90±100% callus on appropriate initiation media and all regenerated shoots from approximately one-half to three-quarters of the calluses produced.

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 98%

Callus formation and plant regeneration from immature and mature embryos of rye (Secale cereale L.)

Ward

Jordan

2001

In Vitro Cell.Dev.Biol.-Plant

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“…Evidence exists that genetic factors are the main contributors to in vitro response to regeneration in crop plants (Hartmann et al, 1989). Genotypic differences in callus induction and plant regeneration ability have been established in several crops including corn (Green et al, 1974;Tomes and Smith, 1985), oats (Cummings et al, 1976;Rines and McCoy, 1981), barley (Hanzel et al, 1985), triticale (Sharma et al, 1980) and wheat (Ahloowalia, 1982). Genetic control of regeneration has been reported to vary from dominant effect of nuclear genes in corn (Hodges et al, 1986) to additive gene action in wheat and rice (Lazar et al, 1984;Miah et al, 1985;Quimio and Zapata, 1990).…”

Section: Introductionmentioning

confidence: 97%

A Study of in vitro Regeneration in Relation to Doses of Growth Regulators in Hybrids of Upland Cotton

Rauf

Rahman

2005

Plant Cell Tiss Organ Cult

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The frequency of in vitro callus induction and plant regeneration is influenced by several factors, including composition of culture medium, explant source, and the genotype. Crosses between regenerable and nonregenerable upland cotton cultivars were evaluated for hybrid vigour towards regeneration responses, which is consequential in recalcitrant crop species like cotton where regeneration is limited only to a few cultivars. The results indicated that regenerable and non-regenerable parental cultivars had similar potential of producing callus, but differed in producing callus weight and embryogenic calli. Mean performance of crosses, regarding callus induction, callus weight, callus growth rate, percent embryo induction, and percentage of germinating embryos, deviated considerably from the performance of their parents, signifying the presence of hybrid vigour for the expression of these traits. Magnitude of hybrid vigour varied across hormonal levels. Genetic component was evident for all the traits although of lower magnitude. The results indicated that genetic component in the phenotypic expression of callus growth, percentages of embryo induction and germinating embryos was higher than that of callus induction, callus weight and percentage of embryogenic calli. Hormonal concentration in the media had affect on the degree of gene expression responsible for regeneration in upland cotton. Over, partial-and additive-dominance types of gene effects were apparent in the expression of these traits. Genotype · growth regulator level interaction caused considerable variation in the expression of regeneration responses, suggesting that determination of specific level of growth regulator concentration in the medium was necessary for a particular genotype to obtain optimum response. Genotype · explant source interaction was, however, relatively less important. Differences among genotypes for percent embryo induction were clearly evident.

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“…Plant tissue culture techniques complement the breeding methods in the widening of genetic variation . In triticale various explants have been used for callus initiation and plant regeneration (Ono & Larter, 1976 ;Sharma et al ., 1980 ;Reddy & Reddy, 1988 ;Padmaja et al ., 1992) . In spite of several reports on callusing and regeneration in triticale, limited information is available on genetic basis of regeneration .…”

Section: Introductionmentioning

confidence: 99%

Genetic basis of plant regeneration in hexaploid triticale

Reddy

1993

Euphytica

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Glume cultures of monogenic recessive mutants PGTSLS1 (Plant Genetics triticale selection large spike 1) and PGTSLG2 (Plant Genetics triticale selection long grain 2) were employed along with their parent PGTS control (Plant Genetics triticale selection control) and their F1 and F2 progenies, to determine the genetic basis of plant regeneration in hexaploid triticale . The mutant PGTSLS1 exhibited greater efficiency of plant regeneration (22 .4%) followed by PGTS control (7 .6%) and PGTSLG2 did not exhibit any regeneration . All the three Fl's exhibited plant regeneration frequency on par with that of control (6 .9-7 .3%), suggesting dominant nature of control genotype over the isogenic mutants . The F2 results suggested that genetic control over the high frequency regeneration of PGTSLS1 was monogenic recessive in nature, and genetic control over the recalcitrant nature of PGTSLG2 also was monogenic recessive . The F2 of the cross PGTSLS1 x PGTSLG2 segregated into four classes . Of the 114 F2 plants, 19 showed no regeneration, 70 of them exhibited 6-8% regeneration, 20 of them 19-24% regeneration, and 5 of them exhibited highest frequency regeneration (57-60%) . These observations suggest dihybrid segregation for regeneration . The highest frequency of plant regeneration (57-60%) exhibited by 5 F2 plants may be due to the interaction of non-allelic genes in recessive condition . These results clearly demonstrate the association of at least two genes with plant regeneration in hexaploid triticale.

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Genotypic differences in organogenesis from callus of ten triticale lines

Cited by 28 publications

References 7 publications

Callus formation and plant regeneration from immature and mature embryos of rye (Secale cereale L.)

Callus formation and plant regeneration from immature and mature embryos of rye (Secale cereale L.)

A Study of in vitro Regeneration in Relation to Doses of Growth Regulators in Hybrids of Upland Cotton

Genetic basis of plant regeneration in hexaploid triticale

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