Genotypic characterization and antimicrobial resistance of Vibrio cholerae and Vibrio parahaemolyticus isolated from milk, dairy products, and humans with respect to inhibitory activity of a probiotic Lactobacillus rhamenosus

Tahoun, Asmaa B.M.B.; Elez, Rasha M.M. Abou; Elsohaby, Ibrahim; Abdellatif, Samah S.; Nada, Hend S.; Abdelfatah, Eman Nabil

doi:10.1016/j.lwt.2021.111930

Cited by 8 publications

(14 citation statements)

References 60 publications

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“…Though the ampicillin resistance in this study was 56.7%, it was the antibiotics to which the isolates from this study were most resistant. Higher levels of cephalosporin resistance were also reported previously 19,20 compared to findings from our research. Lei et al 17 revealed that isolates were susceptible to ceftazidime and cefotaxime, signifying third-generation cephalosporins effective toward V. parahaemolyticus infections.…”

Section: Discussionsupporting

confidence: 82%

“…www.nature.com/scientificreports/ Resistance to ampicillin was reportedly higher among Vibrio species 2,18 compared to those from our study. Ampicillin and ampicillin-sulbactam resistance (100%) reported previously 9,20,23 contradicted ours. The possible rationale for lower ampicillin resistance could be attributed to the different food types, and the hygiene studied.…”

Section: Discussioncontrasting

confidence: 80%

“…The high levels of intermediate susceptibility to antimicrobial drugs portray possible resistance to such drugs. The MAR index from our study ranged from 0 to 0.69, which is lower and contrasts the findings from previous studies 2, 23 and is similar to earlier reports 20 . Ahmed et al 23 reported that all isolates showed resistance to ≥ 7 antibiotics, which is way above our study's findings.…”

Section: Discussionsupporting

confidence: 73%

“…Findings on tetracycline resistance from our study were higher than previously reported 2,17 . Higher levels of quinolone and fluoroquinolone resistance were observed in our study than previously reported 19,20 . Additionally, the isolates from our study exhibited lower resistance to aminoglycosides compared to previous reports 2,4 .…”

Section: Discussioncontrasting

confidence: 60%

“…Prevalence between 3.67 and 4.96% compared to our study has been reported 4,17 . A higher prevalence between 37.5 and 90% has also been documented [18][19][20] . Several outbreaks of V. parahaemolyticus from food in different countries happen through the ingestion of food.…”

Section: Discussionmentioning

confidence: 91%

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Surveillance of Vibrio parahaemolyticus pathogens recovered from ready-to-eat foods

Beshiru

Igbinosa

2023

Sci Rep

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This study examined the occurrence of V. parahaemolyticus from ready-to-eat (RTE) food in Delta State, Nigeria. It also characterized antibiotic resistance and virulence gene profile patterns to determine the associated health risk hazard. Food samples total of 380 were collected randomly and assessed for V. parahaemolyticus. V. parahaemolyticus isolates were characterized for their virulence and antibiogram potentials using a phenotypic and polymerase chain reaction (PCR) approach. A total of 42 (11.1%) samples were contaminated with V. parahaemolyticus. In 17/42 (40.5%) of the V. parahaemolyticus-positive samples, the densities were < 10 MPN/g. However, 19/42 (45.2%) and 6/42 (14.3%) of the samples had densities of 10 – 102 and > 102 MPN/g, respectively. A total of 67 V. parahaemolyticus isolates were identified using PCR; 54(80.6%) isolates were multidrug resistant. A total of 22 (32.8%), 39 (58.2%), and 67 (100%) of the V. parahaemolyticus harbored the tdh, trh, and tlh toxin genes, respectively. The T3SS1 gene (vcrD1) was detected in 67 (100%) of the isolates. The T3SS2α genes which were vcrD2, vopB2, and vopT were detected in 21 (31.3%), 11 (16.4%) and 30 (44.8%) of the isolates respectively. Some of the V. parahaemolytics strains harbored the orf8 gene 20 (29.9%), and a combination of orf8 + tdh genes 12 (17.9%), categorized as pandemic strains. The antibiotic resistance genes detected in this study include blaTEM 33 (49.3), tetM 19 (28.4), cmlA 32(47.8) and sul1 14 (20.9). The concentration levels and prevalence of V. parahaemolyticus in RTE foods indicate contamination of ready-to-eat foods, particularly street foods consumed in the Delta State of Nigeria, threatening public health and consumer safety.

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Section: Discussionsupporting

confidence: 82%

Section: Discussioncontrasting

confidence: 80%