Genotypic and phenotypic profiles of virulence factors and antimicrobial resistance of Proteus mirabilis isolated from chicken carcasses: potential zoonotic risk

Sanches, Matheus Silva; Baptista, Ana Angelita Sampaio; Souza, Marielen de; Menck-Costa, Maísa Fabiana; Koga, Vanessa Lumi; Kobayashi, Renata Katsuko Takayama; Rocha, Sérgio Paulo Dejato da

doi:10.1007/s42770-019-00086-2

Cited by 24 publications

(23 citation statements)

References 68 publications

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“…The gene ireA is responsible for encoding a siderophore receptor, which is considered an important virulence factor in obtaining host Fe 3+ for the maintenance of bacterial metabolism during the infectious process [12]. A study published by our research group characterized the virulence profile of 32 P. mirabilis strains isolated from cellulitis lesions in broiler carcasses and demonstrated that all strains had a diversity of genes associated with P. mirabilis virulence [14].…”

Section: Discussionmentioning

confidence: 99%

“…The isolates were collected with the aid of sterile swabs and transported in Cary-Blair medium (Difco™, USA). Of the 34 P. mirabilis strains recovered from cellulitis in chicken carcasses, 32 were characterized for virulence profile and antimicrobial resistance in another study published by our research group [14] and the other two strains (LBUEL-A33 and LBUEL-A34) were selected for the present study. The strain LBUEL-A33 was chosen because it has a greater number of virulence factors and the strain LBUEL-A34 because it has a smaller number.…”

Section: Samplingmentioning

confidence: 99%

“…The genes mrpA, pmfA, atfA, ucaA (fimbriae), zapA and ptA (proteases), hpmA and hlyA (hemolysins), and ireA (siderophore receptors), which are associated with the virulence of P. mirabilis [9], and ompT (protease), hlyF (hemolysin), iss (serum resistance), iutA and iroN (siderophore receptors), which are associated with the virulence of APEC [15], were detected using polymerase chain reaction (PCR) with the primers described in Table 1. Bacterial DNA was obtained using the boiling extraction method according to Sanches et al [14]. PCR was performed on a GeneAmp® PCR System 9700 thermal cycler (Applied Biosystems, USA) in a final volume of 25 μL and composed of 2 mM MgCl 2 (Invitrogen™), 10× buffer (Invitrogen™), 0.2 mM dNTPs (Invitrogen™), 20 pmol primer forward, 20 pmol primer reverse (Invitrogen™), 1.25 U Taq DNA polymerase (Invitrogen™), DNA template, and Milli-Q ultrapure water (Millipore™).…”

Section: Detection Of Virulence Genesmentioning

confidence: 99%

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Proteus mirabilis causing cellulitis in broiler chickens

et al. 2020

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Given the need to understand the virulence profile of Proteus mirabilis isolates from cellulitis in broiler chickens and their ability to cause lesions, the present study aimed to characterize genotypically and phenotypically the virulence profiles of two strains of P. mirabilis isolated from cellulitis in broilers, as well as to evaluate their ability to experimentally reproduce the lesions in vivo. The strain with the highest virulence potential (LBUEL-A33) possessed mrpA, pmfA, ucaA, atfA (fimbriae), zapA, ptA (proteases), hpmA (hemolysin), and ireA (siderophore) genes, formed a very strong biofilm, and expressed the pattern of aggregative adhesion and cytotoxicity in Vero cells. The strain with the lowest virulence potential (LBUEL-A34) did not present the pmfA and ucaA genes, but expressed the pattern of aggregative adhesion, formed a strong biofilm, and did not show cytotoxicity. Both strains developed cellulitis in an animal model within 24 h post-inoculation (PI), and the degree of lesions was not significantly altered up to 120 h PI. The LBUEL-A33 strain was also inoculated in combination with an avian pathogenic Escherichia coli (APEC 046), and the lesions showed no significant changes from the individual inoculation of these two strains. Histological analysis showed that the LBUEL-A33 strain developed characteristic cellulitis lesions. Thus, both strains of P. mirabilis isolated in our study have several virulence factors and the ability to develop cellulitis in broilers.

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Section: Discussionmentioning

confidence: 99%

Section: Samplingmentioning

confidence: 99%

Section: Detection Of Virulence Genesmentioning

confidence: 99%

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Proteus mirabilis causing cellulitis in broiler chickens

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“…The detection of virulence genes was performed by using PCR. The following genes were analyzed: ptA, zapA, ucaA, ireA [ 30 ], hpmA [ 7 ], mrpA [ 28 ], pmfA [ 36 ], and atfA [ 35 ]. The bacterial DNA was extracted using the boiling method, followed by thermal shock.…”

Section: Methodsmentioning

confidence: 99%

Virulence, resistance and clonality of Proteus mirabilis isolated from patients with community-acquired urinary tract infection (CA-UTI) in Brazil

Oliveira

Barbosa

Faustino

et al. 2021

Microbial Pathogenesis

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Urinary tract infections (UTIs) are among the most common human infections, both in hospitals and in communities. Proteus mirabilis is known to cause community-acquired urinary tract infection (CA-UTI) and is an important causative agent of nosocomial UTIs. The pathogenesis of this species is related to its ability to manifest virulence factors, such as biofilms, adhesion molecules, urease, proteases, siderophores, and toxins. In this study, we investigated the virulence, sensitivity to antimicrobials, and clonal relationship of 183 strains isolated from the urine of CA-UTI patients in Londrina, Paraná State, Brazil. A total of 100% of the strains were positive for hpmA, ptA, zapA, mrpA, pmfA, ireA, and atfA virulence genes. The ucaA gene was positive in 81.4% of the cases. The strains showed high rates of sensitivity to the evaluated antimicrobials, and only one was ESBL-positive. All the tested bacteria showed the capacity to form biofilms: 73.2% had a very strong intensity, while 25.7% had a strong intensity, and 1.1% had a moderate intensity. Regarding clonality, 40 clonal clusters were found among the microorganisms tested. Our results showed that strains of P. mirabilis isolated from CA-UTI patients have several virulence factors. Although the urinary clinical isolates studied showed high sensitivity to antimicrobials, the strains showed a strong capacity to form biofilms, making antibiotic therapy difficult. In addition, it was observed that there were clones of P. mirabilis circulating in the city of Londrina.

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“…All the analyzed contaminant strains recovered from mCCD agar were phenotypically characterized as nonproducing ESBLs Proteus mirabilis. Multidrug-resistant P. mirabilis showing β-lactamases genes have been previously isolated from chicken carcasses ( Sanches et al., 2019 ) and reported on Campylobacter cultures by next-generation DNA sequencing analysis ( Oakley et al., 2012 ).…”

Section: Resultsmentioning

confidence: 99%

Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil

et al. 2020

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Chicken is a leading source of thermotolerant Campylobacter , which triggers human foodborne enteritis. This study evaluated thermotolerant Campylobacter contamination of retail chicken in southern Brazil, using qualitative and quantitative analyses. Selective enrichment in Bolton broth for 24 and 48 h after plating onto modified charcoal–cefoperazone–deoxycholate ( mCCD ) agar and Preston agar was assessed. The combined results of the detection and enumeration methods revealed a frequency of 70% occurrence of thermotolerant Campylobacter in chicken samples. Campylobacter was enumerated in 60% of the samples, whereas 46% of the samples were positive in the qualitative analysis. Quantitative analysis showed average counts of 3.10 ± 0.15 log 10 CFU/sample. Higher numbers of Campylobacter -positive samples were found using 24-h enrichment before plating onto Preston agar (46%) than onto mCCD agar (2%). The majority of isolated strains were identified as Campylobacter jejuni , and Campylobacter coli was also found but to a lesser extent. Subtyping revealed a clear distinction between strains isolated from different chicken sources. The enriched samples plated onto mCCD agar showed extensive spreading of nonproducing extended-spectrum β-lactamases Proteus mirabilis that hampered the identification of Campylobacter colonies. P. mirabilis strains showed resistance to cefoperazone, trimethoprim, and polymyxin B present in broth and plate media used and were inhibited by rifampicin present in Preston agar. The results underline the effect of the spread of contaminant strains on Campylobacter cultures, which might be prevented using a recently revised International Organization for Standardization method for qualitative analysis of chicken.

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Genotypic and phenotypic profiles of virulence factors and antimicrobial resistance of Proteus mirabilis isolated from chicken carcasses: potential zoonotic risk

Cited by 24 publications

References 68 publications

Proteus mirabilis causing cellulitis in broiler chickens

Proteus mirabilis causing cellulitis in broiler chickens

Virulence, resistance and clonality of Proteus mirabilis isolated from patients with community-acquired urinary tract infection (CA-UTI) in Brazil

Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil

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