Genotypic and metabolic approaches towards the segregation of Klebsiella pneumoniae strains producing different antibiotic resistant enzymes

Fatty Acid Methyl Ester (FAME) Profiling Identifies Carbapenemase-Producing Klebsiella pneumoniae Belonging to Clonal Complex 258

“…Taken together, these findings suggest that CC258 and non-CC258 K. pneumoniae isolates differ from one another with respect to fundamental aspects of metabolism, including the metabolism of amino acids (2-phenylacetate) and fatty acids (9(Z)-octadecenoate, hexadecanoate, and pentadecanoate). Such fundamental metabolic differences are in agreement with the conclusions that have been drawn from prior studies, for example [10][11][12]. Figure 2.…”

“…Rees and colleagues demonstrated that CRKP isolates belonging to CC258 produced a distinct volatile metabolic signature relative to both CSKP isolates belonging to other STs, as well as CRKP isolates belonging to non-CC258 lineages [11]. Low and colleagues demonstrated that CRKP and CSKP produced distinct soluble metabolic profiles using a population of resistant isolates that were likely neither CC258 nor KPC-producers based on the geographic region from which they were obtained [12]. Taken together, these findings suggest that carbapenemase-producing K. pneumoniae isolates arising from dominant lineages are transcriptionally and metabolically distinct from non-carbapenemase-producing isolates arising from a diverse range of other, non-dominant genetic lineages.…”

Caracterización molecular y detección de genes blaCTX-M grupos 1 y 9 en Klebsiella pneumoniae resistentes a ceftazidima, en un hospital de San José de Cúcuta, Colombia

“…En un trabajo realizado por Low y cols. (2017), la RPC-REP permitió agrupar en un cluster principal la mayoría de los aislados de K. pneumoniae caracterizados como resistentes a carbapenem 36 . Koroglu y cols., en el año 2015 realizaron un estudio para establecer las relaciones clónicas de 15 aislados de K. pneumoniae empleando RPC-REP y PFGE como métodos de tipificación, concluyendo que el método RPC-REP era claramente más rápido y conveniente que PFGE, pero su poder discriminatorio parece ser inferior al de PFGE 14 .…”

Coffee consumption revealed sex differences in host endogenous metabolism and gut microbiota in healthy adults

“…A standard 1‐dimensional (1D) 1 H‐NMR spectrum was acquired for each sample with a pulse (recycle delay [RD]–90°– t 1 –90°– t m –90°–acquire free induction decay [FID]) on a Bruker 600 MHz spectrometer (Bruker Biospin, Fallenden, Switzerland) equipped with a 5 mm BB(F)O broadband probe operating at 600.13 MHz (ambient probe temperature 27°C). The spectra were acquired according to parameters used previously (Low et al., 2017; Yap et al., 2015). The field frequency was locked on D 2 O solvent and water peak suppression was performed by gradient water pre‐saturation during RD of 4 s and a mixing time ( t m ) of 0.01 s. The 90° pulse length was adjusted to ~10 μs and an acquisition time of 2.65 s was used.…”

“…This study was approved by International Medical University Joint Research and Ethics Committee (IMU MREC number: BP 1-01/12 (09) 2015). The spectra were acquired according to parameters used previously (Low et al, 2017;Yap et al, 2015…”