“…This microorganism is considered pathogenic to P. xylostella, but little bit virulent (Jun 2000), in contrast to the results of this study. This virulence variability can be attributed to genetic diversity of this isolates, based on insect host and geographical region, as observed by Suwannakut et al (2005).…”
The objectives were to evaluate the efficiency of entomopathogenic fungi against Plutella xylostella (L.) and the compatibility of the most virulent isolates with some of the insecticides registered for use on cabbage crops. Pathogenicity tests used isolates of Beauveria bassiana, Metarhizium rileyi, Isaria fumosorosea, Isaria sinclairii, and Lecanicillium muscarium standardized at a concentration of 10(7) conidia/ml. Cabbage leaf discs were immersed in these suspensions, and after evaporation of the excess water, were placed 10 second-instar larvae of P. xylostella, totaling 10 leaf discs per treatment. Mortality was assessed 7 d after treatment, and the isolates that caused mortality>80% were used to estimate LC50 and LT50. The compatibilities of the most virulent isolates and the insecticides were tested from the mixture of these into the culture medium, and after solidifying, the medium was inoculated with an aliquot of the isolated suspension. The following parameters were evaluated: growth of the colony, number and viability of conidia after 7 d. The isolated IBCB01, IBCB18, IBCB66, and IBCB87 of B. bassiana, LCMAP101 of M. rileyi, and ARSEF7973 of I. sinclairii caused mortality between 80 and 100%, with LC50 and LT50 between 2.504 to 6.775×10(4) conidia/ml and 52.22 to 112.13 h, respectively. The active ingredients thiamethoxam and azadirachtin were compatible with the entomopathogenic fungi. The results suggest that the use of these isolates is an important alternative in the pesticidal management of P. xylostella, with the possible exception of the associated use of chemical controls using the active ingredients thiamethoxam or azadirachtin.
“…This microorganism is considered pathogenic to P. xylostella, but little bit virulent (Jun 2000), in contrast to the results of this study. This virulence variability can be attributed to genetic diversity of this isolates, based on insect host and geographical region, as observed by Suwannakut et al (2005).…”
The objectives were to evaluate the efficiency of entomopathogenic fungi against Plutella xylostella (L.) and the compatibility of the most virulent isolates with some of the insecticides registered for use on cabbage crops. Pathogenicity tests used isolates of Beauveria bassiana, Metarhizium rileyi, Isaria fumosorosea, Isaria sinclairii, and Lecanicillium muscarium standardized at a concentration of 10(7) conidia/ml. Cabbage leaf discs were immersed in these suspensions, and after evaporation of the excess water, were placed 10 second-instar larvae of P. xylostella, totaling 10 leaf discs per treatment. Mortality was assessed 7 d after treatment, and the isolates that caused mortality>80% were used to estimate LC50 and LT50. The compatibilities of the most virulent isolates and the insecticides were tested from the mixture of these into the culture medium, and after solidifying, the medium was inoculated with an aliquot of the isolated suspension. The following parameters were evaluated: growth of the colony, number and viability of conidia after 7 d. The isolated IBCB01, IBCB18, IBCB66, and IBCB87 of B. bassiana, LCMAP101 of M. rileyi, and ARSEF7973 of I. sinclairii caused mortality between 80 and 100%, with LC50 and LT50 between 2.504 to 6.775×10(4) conidia/ml and 52.22 to 112.13 h, respectively. The active ingredients thiamethoxam and azadirachtin were compatible with the entomopathogenic fungi. The results suggest that the use of these isolates is an important alternative in the pesticidal management of P. xylostella, with the possible exception of the associated use of chemical controls using the active ingredients thiamethoxam or azadirachtin.
“…As mortalidades confirmadas para as diferentes concentrações do isolado UFMS 03 apresentaram correlação polinomial positiva com a concentração de conídios viáveis (Figura 3). A diferença na origem do isolados e do hospedeiro que foi infectado resulta em diferentes valores de patogenicidade (Suwannakut, Boucias & Wiwat, 2005).…”
This work aimed to evaluate the pathogenicity of the UFMS 03 strain of Metarhizium rileyi to the Spodoptera cosmioides caterpillars under laboratory conditions. The experimental trial consisted of a completely randomized design, with six treatments. Each treatment consisted of 10 repetitions, containing five S. cosmioides caterpillars, standardized in 3 mm in length. The S. cosmioides caterpillars were placed inside Petri dishes and sprayed with the aid of an adapted Poter Tower, at a pressure of 15 pounds / inch, using 1 mL of the concentrations 1.0×1010, 1.0×109, 1.0×108, 1.0×107 and 1.0×106 conidia mL-1. Afterwards, the plates were sealed with pvc film and placed in an air-conditioned chamber at 25±1 °C, 70±10% RH, and a 12-hour photophase. Mortality was verified 24 hours after spraying. The concentrations of the M. rileyi strain UFMS 03 proved to be efficient, with emphasis on the concentration 1.0×108 conidia mL-1 showing 98% efficiency, in less lethal time (14.11 days). These results show the strain UFMS 03 of M. rileyi as a control agent for S. cosmioides.
“…Metarhizium rileyi (Farlow) Kepler frequently causes natural epizootics in lepidopteran populations, especially from the Noctuidae family, in different subtropical and temperate agroecosystems, reducing larval populations, which makes it an excellent biocontroller for its high virulence (Boucias et al, 2000;Inglis et al, 2001;Devi et al, 2003;Suwannakut et al, 2005). The aim of the study was to determine the natural occurrence of M. rileyi on S.…”
Section: Obtención De Parasitoidesmentioning
confidence: 99%
“…Its conventional control involves the application of chemical insecticides. However, the incorrect use of these pesticides has caused multiple problems of intoxication towards farmers and generated resistance by the pest (Tinoco & Halperin, 1998;Ahmad et al, 2007 (Boucias et al, 2000;Inglis et al, 2001;Devi et al, 2003;Suwannakut et al, 2005).…”
Temperature, relative humidity (RH) and accumulated precipitation were obtained from the weather stations of Unifrut and INIFAP. The highest percentage (48 %) of mycosed larvae by M. rileyi was obtained during the sample performed in August, where temperature and RH average were 16.76 °C and 80.1 %, respectively, and an accumulated precipitation of 218 mm. These results suggest that the climatic conditions were optimal for the development of the fungal epizootic on field.
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