Genotypes of Mycobacterium bovis strains isolated from domestic animals and wildlife in Canada in 1985–2015

Mycobacterium bovis Persistence in Two Different Captive Wild Animal Populations in Germany: a Longitudinal Molecular Epidemiological Study Revealing Pathogen Transmission by Whole-Genome Sequencing

“…In contrast, the VNTR loci 3690 (3 repeats) and 4052 (3 repeats) in our study differed from the patterns reported from Canada (locus 3690, 2 repeats; locus 4052, 2, 4, and 5 repeats [48]), while they were concordant with the results reported in the South African study (49). VNTR locus 2163a, with four repeats in the present study, differed remarkably from locus 2163a of M. bovis isolates described in a previous study from South Africa (49) and in a Spanish study (50), with more than eight repeat units in both studies, while it was not reported in the Canadian study (48). The route of original introductions of this strain to the animal park and the captive deer farm or between these two settings could not be revealed, and trading contacts or other forms of interrelationships between them could not be verified.…”

“…With respect to the 24 standardized VNTR loci, the allele type pattern identified in the present study was mainly concordant with the patterns published in previous studies of genotypes of M. bovis isolates from Canada, with 137 isolates collected over 30 years (48), and South Africa, with 131 isolates collected over 15 years (49). The two VNTR loci with variations in our study, locus 2165 and locus 2461, were shown in these publications to have discriminatory indexes (HGDIs) of 0.741 (48) and 0.30 (49) for locus 2165 and 0.342 (48) and 0.50 (49) for locus 2461. But even these allele type variants of our strains were in agreement with the two previous reports.…”

Genome Sequences of Five Mycobacterium bovis Strains Isolated from Farmed Animals and Wildlife in Canada

“…In Canada, bTb has essentially been eliminated from livestock but remains in two wildlife reservoirs, Wood Buffalo National Park (WBNP) and Riding Mountain National Park (RMNP) ( 2 ). Whole-genome sequencing was performed on five M. bovis strains representing major genotype clusters observed in Canadian animal farms and wildlife between 1985 and 2015 ( 3 ) to provide reference information for epidemiologic investigations of bTb outbreaks and for comparative genomics studies. Strain 2002/0476 was isolated from cattle in the province of Ontario in 2002, strain 2006/0077 was recovered from an elk farm in Ontario in 2006, and strain 2011/0565 was recovered from cattle in British Columbia in 2011.…”

“… M. bovis isolates genotyped by conventional methods ( 3 ) ( Table 1 ) were grown on Middlebrook 7H11 agar for 4 weeks, and genomic DNA was extracted using the MasterPure Gram-positive DNA purification kit (Epicentre, USA). Sequencing libraries for M. bovis 2011/0565 and BMR25/85 were prepared using the Nextera XT DNA library preparation kit (Illumina, Inc., USA) and sequenced on a MiSeq sequencer (Illumina, Inc.) using the MiSeq reagent kit v2 to generate 2 × 250-bp paired-end sequences.…”

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“…Argentina (ZUMÁRRAGA et al, 2013;GHAVIDEL et al, 2018 Estudos anteriores no Brasil também relataram esse espoligotipo (ROCHA et al, 2013;CARVALHO et al, 2016). Esse perfil já foi detectado na Ásia, Europa e na Américas, sendo um dos espoligotipos mais disperso pelo mundo (ANDRIEVSKAIA et al, 2018;GHAVIDEL et al, 2018).…”