Genotype MTBDR plus assay for molecular detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis

Sharma, Soniya; Madan, Molly; Agrawal, Chaturbhuj; Asthana, A. K.

doi:10.4103/0377-4929.138738

Cited by 6 publications

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“…The most common mutation associated with rifampicin resistance was S531L mutation in the rpoB region consistent with the findings of various other studies [ 12 – 15 ].…”

Section: Discussionsupporting

confidence: 91%

“…This study showed low rates (1%) of MDR TB among new sputum positive pulmonary TB patients similar to other Indian studies [ 9 , 10 ]. The prevalence of INH monoresistance ranging from 1% to 7% has been reported in various Indian studies [ 11 , 12 ] and our study population showed 1.5% INH monoresistance.…”

Section: Discussionsupporting

confidence: 64%

“…In this study, the common mutation associated with INH resistance was C15T in the -15 nucleic acid position of the promoter region of inhA gene. The prevalence of mutations in the katG and inhA genes seems to vary widely in different geographic locations as reported in various studies conducted in different countries [ 12 , 22 , 23 ]. In a study by Maurya and et al [ 24 ], the most prominent mutations in rpoB , katG , and inhA genes were S531L, S315T1, and C15T, respectively, similar to this study.…”

Section: Discussionmentioning

confidence: 99%

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Primary Multidrug Resistant Tuberculosis and Utility of Line Probe Assay for Its Detection in Smear-Positive Sputum Samples in a Tertiary Care Hospital in South India

Yacoob

Jose

Lelitha

et al. 2016

Journal of Pathogens

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In a high tuberculosis burdened country like India, rapid, cost-effective, and reliable diagnostic tools for tuberculosis are an urgent need of the hour to prevent inappropriate treatment strategies and further spread of resistance. This study aimed to estimate the proportion of new smear-positive tuberculosis cases with primary resistance to rifampicin and/or isoniazid as well as identify the common mutations associated with it. Sputum of 200 newly diagnosed smear-positive cases of 1+ score and above was directly subjected to Line Probe Assay using the GenoType MTBDRplus assay kit. All samples were inoculated onto solid media and 61 samples were inoculated in automated liquid culture also. The Line Probe Assay gave hundred percent interpretable results with 2.5% of the study population showing resistant pattern. Only 1% of the cases were primary multidrug resistant tuberculosis and 1.5% showed isoniazid monoresistance. S531L and C15T were the most common genetic mutations seen for rifampicin and isoniazid resistance, respectively. 40% had absent rpoB wild type 8 band indicating probable silent mutation after clinical correlation. The average turnaround time for Line Probe Assay was far less (3.8 days) as compared to solid and liquid cultures (35.6 days and 13.5 days, resp.).

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“…The most common mutation associated with rifampicin resistance was S531L mutation in the rpoB region consistent with the findings of various other studies [ 12 – 15 ].…”

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Primary Multidrug Resistant Tuberculosis and Utility of Line Probe Assay for Its Detection in Smear-Positive Sputum Samples in a Tertiary Care Hospital in South India

Yacoob

Jose

Lelitha

et al. 2016

Journal of Pathogens

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“…Most of the isolates had S315T and S531L mutations in the katG and rpoB genes, respectively. This is in line with what was observed among MDR isolates by using not only line-probe assays (69.8 vs. 55.9–90.6% for S315T katG; 64.1 vs. 41.4–67.2% for S531L rpoB ) (Farooqi et al, 2012 ; Shubladze et al, 2013 ; Sharma et al, 2014 ; Javaid et al, 2016 ; Spinato et al, 2016 ) but also sequencing strategy (66–76% for S315T katG; 46.3–66% for S531L rpoB ) (Jou et al, 2005 ; Ali et al, 2011 ; Makadia et al, 2012 ; Jagielski et al, 2015 ; Unissa et al, 2015 ). Both these mutations are thought to be low fitness cost mutations, with no adverse effect on transmission capacity (Gagneux, 2009 ).…”

Section: Discussionsupporting

confidence: 87%

Evaluation of Genotype MTBDRplus and MTBDRsl Assays for Rapid Detection of Drug Resistance in Extensively Drug-Resistant Mycobacterium tuberculosis Isolates in Pakistan

et al. 2018

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Pakistan ranks 5th among the world's highest tuberculosis (TB) burden countries alongside the 6th among countries with the highest burden of drug-resistant TB, including multi-drug resistant (MDR)-TB. Methods for rapid and reliable drug susceptibility testing (DST) are prerequisite for the prompt institution of effective anti-TB treatment. The aim of this study was to evaluate the efficiency of Genotype MTBDRplus and MTBDRsl assays for the detection of MDR and (pre-) extensively drug-resistant (XDR-TB) isolates in Pakistan. The study included 47 pre-XDR and 6 XDR-TB isolates, recovered from 53 patients from Pakistan. Conventional DST was performed using the standard 1% proportion method on the Löwenstein-Jensen medium. For molecular determination of drug resistance, GenoType MTBDRplus and GenoType MTBDRsl assays (Hain Lifescience, Germany) were used. To evaluate discrepancies between conventional and molecular DST results, mutation profiling was performed by amplifying and sequencing seven genetic loci, i.e., katG, inhA, and mabA-inhA promoter, rpoB, gyrA, embB, rrs. The sensitivity of Genotype MTBDRplus was 71.7% for isoniazid (INH) and 79.2% for rifampicin (RIF). Sequence analysis revealed non-synonymous mutations in 93.3 and 27.3% of isolates phenotypically resistant to INH and RIF, respectively, albeit susceptible when tested by GenoType MTBDRplus. GenoType MTBDRsl had a sensitivity of 73.6, 64.7, 20, 25, and 100% for the detection of fluoroquinolones, ethambutol, kanamycin, amikacin, and capreomycin resistance, respectively. Upon sequencing, mutations were detected in 20, 77.8%, and all isolates phenotypically resistant to aminoglycosides, ethambutol, and fluoroquinolones, respectively, yet declared as susceptible with GenoType MTBDRsl. Low sensitivities seriously impede the large-scale application of the Genotype MTBDRplus and MTBDRsl assays. Unless further optimized, the currently available line-probe assays should rather be auxiliary to the conventional, phenotype-based methods in the detection of MDR- and XDR-TB in Pakistan.

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“…The main difference in these latter findings may show our inclusion of all forms of presumptive tuberculosis while other studies included identified cases of Mycobacterium tuberculosis . In contrast, our finding was higher when compared with studies conducted in Metema and Armacho (5.7%) [ 7 ], Felege Hiwot Referral Hospital, and Debre Tabor Hospital (14.6%) [ 25 ], in three referral hospitals and the regional laboratory in Addis Ababa (15.11%) [ 26 ], other parts of Ethiopia (4.7–10.8%) [ 27 – 29 ], Nigeria (10.3%) [ 30 ] and India (2.31%) [ 31 ]. The variations might be due to the difference in study design, type and number of participants, and environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

Trends of Mycobacterium tuberculosis and rifampicin resistance in Adigrat General Hospital, Eastern zone of Tigrai, North Ethiopia

Abay

Abraha

2020

Trop Dis Travel Med Vaccines

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Background: Tuberculosis is an infectious disease usually caused by Mycobacterium tuberculosis bacteria. The global emergence of mono-or multidrug-resistant tuberculosis and extensively drug-resistant forms of tuberculosis pose a considerable challenge to tuberculosis control programs. There has been no reliable and organized data on trends and drug resistance of Mycobacterium tuberculosis in the study area. Therefore, this study aimed to determine the trends of Mycobacterium tuberculosis and rifampicin resistance in the Adigrat General Hospital, eastern Zone of Tigrai, North Ethiopia. Methods: A hospital-based retrospective cross-sectional study was conducted at Adigrat General Hospital from January 2015 to 2018.Data was collected retrospectively from the GeneXpert™ TB registration book using a data extraction format. Data was entered into Epi-Info 3.1 and subsequently exported and analyzed using SPSS Version 20.The results were summarized using descriptive statistics, tables, and figures. Bivariate and multi-variant regression analysis was employed to measure the association between dependent and independent variables. P values < 0.05 were considered statistically significant. Result: A total of 5944 Mycobacterium tuberculosis presumptive patients were included in the study. The majority of the study participants were male (58.1%) with participants' median age of 40.0 (IQR 26-57) years, the majority were 30-44 years. The overall positive cases of Mycobacterium tuberculosis was 24.3% (1446) with a total of 132 (9.1%) found to be resistant to rifampicin. Of the total confirmed positive cases 8.7% (103/1188) and 11.2% (29/258) were rifampicin resistance of presumptive tuberculosis and presumptive drug resistance tuberculosis patients respectively. Age, the reason for diagnosis, site of presumptive tuberculosis, and/or being HIV infected showed significant association with our dependent variable; however, only age and being HIV infected were associated with rifampicin resistance. Conclusion: In our study, the overall trends of Mycobacterium tuberculosis and rifampicin resistance were found to be high. Rifampicin resistance is more common in patients with HIV and presumptive drug resistance tuberculosis individuals. Therefore, maximizing early detection of drug-resistant and strengthening tuberculosis infection control activities are recommended to reduce the burden of this contagious and potentially deadly disease.

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Genotype MTBDR plus assay for molecular detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis

Cited by 6 publications

References 0 publications

Primary Multidrug Resistant Tuberculosis and Utility of Line Probe Assay for Its Detection in Smear-Positive Sputum Samples in a Tertiary Care Hospital in South India

Primary Multidrug Resistant Tuberculosis and Utility of Line Probe Assay for Its Detection in Smear-Positive Sputum Samples in a Tertiary Care Hospital in South India

Evaluation of Genotype MTBDRplus and MTBDRsl Assays for Rapid Detection of Drug Resistance in Extensively Drug-Resistant Mycobacterium tuberculosis Isolates in Pakistan

Trends of Mycobacterium tuberculosis and rifampicin resistance in Adigrat General Hospital, Eastern zone of Tigrai, North Ethiopia

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