Genotype diversity of Bacillus anthracis strains isolated from the Caucasus region

Еременко, Е. И.; Ryazanova, A. G.; Tsygankova, O. I.; Цыганкова, Е. А.; Buravtseva, Nina; Kulitchenko, A. N.

doi:10.3103/s0891416812020024

Cited by 11 publications

(3 citation statements)

References 14 publications

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“…In recent years, Russian and foreign authors have published a substantial number of papers on the design of viable approaches to genotyping anthrax agent strains (Le Flèche et al, 2001;Keim et al, 2004;Van Ert et al, 2007;Gierczynski et al, 2009;Eremenko et al, 2012;Afanas'ev et al, 2014). Most of the available B. anthracis genotyping methods are based on polymorphism of tandem repeats or point mutations in their genome.…”

Section: Discussionmentioning

confidence: 99%

<i>Bacillus anthracis</i> strain differentiation based on SNP and VNTR loci

Anisimova¹,

Fakhrutdinov²,

Mirgazov³

et al. 2022

Vestn. VOGiS

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Bacillus anthracis is the anthrax causative agent. For its epidemiology, it is important not only to identify the etiological agent but also to determine the patterns of its evolution and spread. Modern methods of molecular biology make it possible to detect a number of genetic markers suitable for indicating and differentiating the strains of B. anthracis, including the loci arranged as variable number tandem repeats (VNTRs) and SNPs, one nucleotide-sized differences in the DNA sequence of the loci being compared. The objective of the present study was to examine the effectiveness of SNP analysis and PCR amplif ication of VNTR loci combined with the high-resolution amplicon melting analysis for identif ication and differentiation of the anthrax agent strains. In the study, seven strains of B. anthracis obtained from soil samples and animal carcasses were investigated using vaccine strain STI-1 as a reference. For molecular genetic characterization of these bacteria, analysis of 12 SNPs and variability analysis of eight VNTR loci were carried out. To detect the differences between the strains, their PCR product melting points were measured in the presence of the EvaGreen (Sintol, Russia) intercalating dye. For SNP detection, a PCR assay with double TaqMan probes was applied. It was found that the studied virulent strains, except for B. anthracis No. 1 and 3, could not be attributed to any phylogenetic subgroup of the anthrax agents. The proposed method made it possible to differentiate four out of the seven investigated strains. Strains No. 5–7 had identical SNP and HRM prof iles and, as a result, formed a single cluster. Our investigation has conf irmed that the proposed method can be successfully used for preliminary analysis of an epizootic situation in the case of anthrax.

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Section: Discussionmentioning

confidence: 99%

<i>Bacillus anthracis</i> strain differentiation based on SNP and VNTR loci

Anisimova¹,

Fakhrutdinov²,

Mirgazov³

et al. 2022

Vestn. VOGiS

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“…However, isolates from the Bhutan outbreak were found to be part of the multilocus variable-number tandem repeat analysis B1 lineage (genotype 83) and canonical single-nucleotide polymorphism subgroup B.Br.001/002 ( Figure 1 ) ( 10 – 13 ). The B lineage is less widespread and primarily associated with South Africa, but it has been reported in parts of the United States, Europe, and Asia, including the Caucasus region in a recent report ( 10 – 14 ). The team in Bhutan investigated the mode of B. anthracis transmission and spread among livestock and humans in outbreak areas and implemented control measures.…”

Section: The Studymentioning

confidence: 99%

Investigation and Control of Anthrax Outbreak at the Human–Animal Interface, Bhutan, 2010

Thapa¹,

Tenzin²,

Wangdi³

et al. 2014

Emerg. Infect. Dis.

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“…The research of anthrax infection and its causal agent has been the subject of numerous works by researchers all over the world, but despite the long history of research of B. anthracis, some of the properties of this pathogen remain poorly understood. Among them is the dependence on tryptophan in a number of strains whose virulence is reduced [3].…”

Section: Introductionmentioning

confidence: 99%

Variability of the Bacillus anthracis tryptophan operon

Еременко¹,

Bobrysheva²,

Писаренко³

et al. 2020

Preprint

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BackgroundBacillus anthracis is a causal agent of a zoonotic disease relevant for many countries, and is an agent of bioterrorism. Meanwhile, the reasons for the dependence on tryptophan of some strains with altered virulence have not been established with an almost complete absence of information on the tryptophan operon of this pathogen. In this study, we report gene variability and the structure of the tryptophan operon in B. anthracis strains of the three main lineages.ResultsFor in silico analysis we used 112 B. anthracis genomes, including 68 of those available at the GenBank database and 44 sequenced at our institute. The B. anthracis tryptophan operon has an ancestral structure with a complete set of seven partially overlapping genes. The results show that the variability of all seven tryptophan operon genes is determined by the presence of single nucleotide polymorphisms and InDels. The trpA genes of strains of the main lineage B and trpG genes of strains of the C lineage are pseudogenes and the proteomes lack the corresponding enzymes of the biosynthetic pathway, which may explain the dependence of the strains of line B on tryptophan. ConclusionIn this study, the differences in tryptophan operon genes for B. anthracis strains belonging to different main lineages were demonstrated for the first time. Mutation in the gene of the tryptophan synthase subunit alpha can explain the dysfunction of this enzyme and the dependence on tryptophan in strains of the main lineage B. Identified features suggest a further study of the dependence on tryptophan in B. anthracis strains of the main lineage B and may be of interest from the point of view of intraspecific evolution of the anthrax pathogen.

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Genotype diversity of Bacillus anthracis strains isolated from the Caucasus region

Cited by 11 publications

References 14 publications

<i>Bacillus anthracis</i> strain differentiation based on SNP and VNTR loci

<i>Bacillus anthracis</i> strain differentiation based on SNP and VNTR loci

Investigation and Control of Anthrax Outbreak at the Human–Animal Interface, Bhutan, 2010

Variability of the Bacillus anthracis tryptophan operon

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