Genotype detection of fimH gene of Acinetobacter baumannii isolated from different clinical cases

Abstract

Background: Forty isolates were obtained of Acinetobacter baumannii from (200) samples, the samples were collected from different cases including: - wounds, burns, Stool, Urinary tract infection urine.
 Aim of study: The aim of study is to isolate and diagnose Acinetobacter baumanniii from different clinical cases and to investigate the fimH gene.
 Objective: Respiratory tract infection and blood sample.For the period between 1/9/2016 to 30/11/2016.
 Materials and methods: Identification of 40 isolates confirmed to be Acinetobacter baumannii included 9 isolates from blood, 1 isolate from urinary tract infections, 4 isolates from each of wound infections and Respiratory tract infection, 8 isolates from burns and 14 isolates from stool sample.
 Results: The result revealed that the fimH gene was present in (20) isolates (50%) of Acinetobacter baumannii. The result Showed (7 (isolate (17.5%) of Stool and blood has fimH gene to each of them and Burns (3) isolates (7.5%) has fimH gene and (2) isolate (5%) of respiratory tract infection has fimH gene and finally only (1) isolate (2.5 %) of Wound infection. While all isolated for each of the urinary tract infections doesn't the fimH gene. The gel electrophoresis showed that the molecular weight of fimH gene was 508 bp. 
 Conclusion: Sequential analysis detection of fimH showed three silent mutations that did not affect the amino acid translation.

Factors mediating Acinetobacter baumannii biofilm formation: Opportunities for developing therapeutics

“… 70–92% Silva et al., 2021 ; Zeighami et al., 2019 bla PER-1 Β-lactamase Strains harbouring bla PER-1 gene showed increased biofilm formation compared to strains where it was absent. 30.2% Liu et al., 2016 FimH Type I Fimbriae protein Bacterial cell adhesion 6.8 – 50% Abdullah and Ahmed, 2019 ; Bentancor et al., 2012 ; Padmaja et al., 2020 epsA Polysaccharide export outer membrane protein Potentially plays a role in the export of polysaccharides during biofilm formation. 95% Russo et al., 2010 ; Zeighami et al., 2019 Ptk/wzc Putative tyrosine kinase Plays a role in K1 capsular polysaccharide production 95% Russo et al., 2010 ; Zeighami et al., 2019 csgA Curli specific gene A Amyloidogenic proteins contribute to matrix formation 20.54%−70% Jaisankar et al., 2020 kpsMII Group 2 capsule synthesis Production of capsule 57–75% Kanaan et al., 2021 ; Zeighami et al., 2019 , Pap Pili system Homologous to E. coli pili and associated with A. baumannii biofilm formation 80% Kanaan et al., 2021 ; Li et al.…”