Genotype-dependent expression of specific members of potato protease inhibitor gene families in different tissues and in response to wounding and nematode infection

Expression profile of jasmonic acid-induced genes and the induced resistance against the root-knot nematode (Meloidogyne incognita) in tomato plants (Solanum lycopersicum) after foliar treatment with methyl jasmonate

“…Various PIs isolated from cowpea, sweet potato, and potato inhibited reproduction, growth and development of nematodes (Urwin et al, 1998;Cai et al, 2003;Vishnudasan et al, 2005). The genes of PI are also expressed in a tissue-and genotype-dependent manner after wounding and nematode invasion (Turra et al, 2009). Similarly cystatins (cysteine proteinase inhibitors) show the effect for nematode resistance.…”

Coordinate expression of AOS genes and JA accumulation: JA is not required for initiation of closing layer in wound healing tubers

“…JA has been shown to be capable of inducing accumulation of 3-hydroxy-3-methylglutarylcoenzyme A reductase transcripts, steroid-glycoalkaloids, kunitztype proteinase inhibitors and expansion of cells in tuber discs, i.e. wound-responding tuber tissue (Yamagishi et al, 1993;Choi et al, 1994;Takahashi et al, 1995;Turra et al, 2009). Although it has been shown that JA accumulation is rapidly induced after tuber wounding (Koda and Kikuta, 1994;Lulai and Suttle, 2009), it has not been determined if wound-induced JA is involved in the signaling of critical wound-healing processes such as the initiation of SPP accumulation as part of closing layer development.…”

A maternally inherited DNA marker, descended from Solanum demissum (2n = 6x = 72) to S. tuberosum (2n = 4x = 48)

“…The following thermal profile was used: 10 min at 95°C, 30 cycles of 30 sec at 95°C, 30 sec at 60°C and 1 min at 72°C, followed by a final extension step of 5 min at 72°C. To confirm the absence of genomic DNA contaminations in cDNA samples, PCR was carried out under the same condition with primers Tubf and Tubr (Table 2), which amplified a 525 bp β-tubulin gene fragment from cDNA instead of an approximately 1,600 bp fragment from genomic DNA containing an intron in potato (Turra et al 2009). …”

“…aPrimer sequences for amplification of GBSS and β-tubulin were obtained from Takeuchi et al (2009) and Turra et al (2009), respectively.bcDNA size (Turra et al 2009). …”