Coordinate expression of AOS genes and JA accumulation: JA is not required for initiation of closing layer in wound healing tubers

Lulai, Edward C.; Huckle, Linda L.; Neubauer, Jonathan D.; Suttle, Jeffrey C.

doi:10.1016/j.jplph.2010.12.001

Cited by 31 publications

(14 citation statements)

References 33 publications

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“…On the other hand, injury of potato tubers triggers a rapid increase (by 5-fold) of the basal JA content which peaks 4–6h after wounding and thereafter returns to basal levels, a pattern compatible with a role in the early wound response (Koda and Kikuta, 1994). However, Lulai et al (2011) showed no effect of JA treatment or inhibition of JA accumulation on suberin biosynthesis in the wound closing layer, in agreement with the lack of an enhancing or inhibiting effect of JA with regard to FHT induction (Fig. 8B).…”

Section: Discussionsupporting

confidence: 82%

“…Abscisic acid (ABA) is a potent phytoregulator that reduces evapotranspiration and hastens the wound-associated deposition of suberin (Soliday et al , 1978; Lulai et al , 2008), in contrast to ethylene which is not required for wound suberization (Lulai and Suttle, 2004, 2009). Furthermore, jasmonic acid (JA) is rapidly induced by wounding, but neither JA treatment nor inhibition of JA accumulation have any effect on suberin deposition (Lulai et al , 2011). Clarifying the effects of plant hormones in wound-associated suberization may contribute further to better understanding of the healing processes and might help to improve the quality and storage life of potatoes.…”

Section: Introductionmentioning

confidence: 99%

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The potato suberin feruloyl transferase FHT which accumulates in the phellogen is induced by wounding and regulated by abscisic and salicylic acids

Boher

Serra

Soler

et al. 2013

Journal of Experimental Botany

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The present study provides new insights on the role of the potato (Solanum tuberosum) suberin feruloyl transferase FHT in native and wound tissues, leading to conclusions about hitherto unknown properties of the phellogen. In agreement with the enzymatic role of FHT, it is shown that its transcriptional activation and protein accumulation are specific to tissues that undergo suberization such as the root boundary layers of the exodermis and the endodermis, along with the tuber periderm. Remarkably, FHT expression and protein accumulation within the periderm is restricted to the phellogen derivative cells with phellem identity. FHT levels in the periderm are at their peak near harvest during periderm maturation, with the phellogen becoming meristematically inactive and declining thereafter. However, periderm FHT levels remain high for several months after harvest, suggesting that the inactive phellogen retains the capacity to synthesize ferulate esters. Tissue wounding induces FHT expression and the protein accumulates from the first stages of the healing process onwards. FHT is up-regulated by abscisic acid and down-regulated by salicylic acid, emphasizing the complex regulation of suberin synthesis and wound healing. These findings open up new prospects important for the clarification of the suberization process and yield important information with regard to the skin quality of potatoes.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

The potato suberin feruloyl transferase FHT which accumulates in the phellogen is induced by wounding and regulated by abscisic and salicylic acids

Boher

Serra

Soler

et al. 2013

Journal of Experimental Botany

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“…The role of other phytohormones, including jasmonates, salicylic acid and ethylene, in wound-induced suberization in potato remains speculative Suttle, 2004, 2009;Lulai et al, 2008). That is, the application of inhibitors of ethylene synthesis had no impact on suberin deposition and, while jasmonate levels changed transiently, no specific role in suberization was identified (Lulai et al, 2011). More recently, Barberon et al (2016) demonstrated a role for ABA in the developmental deposition of Arabidopsis root suberin, and provided evidence for interference in the process by ethylene.…”

Section: Aba Levels Are Dynamic During Would Healingmentioning

confidence: 99%

Differential induction of polar and non‐polar metabolism during wound‐induced suberization in potato (Solanum tuberosum L.) tubers

et al. 2018

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SUMMARYWound-induced suberin deposition involves the temporal and spatial coordination of phenolic and fatty acid metabolism. Phenolic metabolism leads to both soluble metabolites that accumulate as defense compounds as well as hydroxycinnamoyl derivatives that form the basis of the poly(phenolic) domain found in suberized tissue. Fatty acid metabolism involves the biosynthesis of very-long-chain fatty acids, 1-alkanols, x-hydroxy fatty acids and a,x-dioic acids that form a poly(aliphatic) domain, commonly referred to as suberin. Using the abscisic acid (ABA) biosynthesis inhibitor fluridone (FD), we reduced wound-induced de novo biosynthesis of ABA in potato tubers, and measured the impact on the expression of genes involved in phenolic metabolism (StPAL1, StC4H, StCCR, StTHT), aliphatic metabolism (StCYP86A33, StCYP86B12, StFAR3, StKCS6), metabolism linking phenolics and aliphatics (StFHT) or acyl chains and glycerol (StGPAT5, StGPAT6), and in the delivery of aliphatic monomers to the site of suberization (StABCG1). In FD-treated tissue, both aliphatic gene expression and accumulation of aliphatic suberin monomers were delayed. Exogenous ABA restored normal aliphatic suberin deposition in FD-treated tissue, and enhanced aliphatic gene expression and poly(aliphatic) domain deposition when applied alone. By contrast, phenolic metabolism genes were not affected by FD treatment, while FD + ABA and ABA treatments slightly enhanced the accumulation of polar metabolites. These data support a role for ABA in the differential induction of phenolic and aliphatic metabolism during wound-induced suberization in potato.

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“…Nevertheless, the specific role of ABA in the regulation ⁄ promotion of wound-induced gene expression at the stem scar tissue of tomato fruits remains to be elucidated. Future experimental approaches need to address the functional involvement and interplay of other regulatory molecules, such as jasmonic acid, salicylic acid, auxin, ethylene and nitric oxide (León et al, 2001;París et al, 2007;Lulai et al, 2011), in the wound responses at the tomato fruit stem scar tissue.…”

Section: Importance Of Endogenous Aba In Wound Healingmentioning

confidence: 99%

Abscisic acid mediates the formation of a suberized stem scar tissue in tomato fruits

et al. 2012

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Summary• During harvest, fleshy berry tomato fruits (Solanum lycopersicum) were wounded at their stem scar. Within 3 d, this wound was rapidly sealed by a process covering the wound site with a membranous layer which effectively protects the tomato fruit from excessive water loss, nutrient elution and the entry of pathogens.• Chemical analysis of the de novo synthesized stem scar tissue revealed the presence of aromatic and aliphatic components characteristic of the biopolyester suberin.• Gene expression patterns associated with suberization were identified at the stem scar region. Changes in the relative abundance of different transcripts suggested a potential involvement of the plant hormone abscisic acid (ABA) in the wound-healing processes.• The amount of ABA present in the stem scar tissue showed a significantly increased level during wound healing, whereas ABA-deficient mutants notabilis, flacca and sitiens were largely devoid of this rise in ABA levels. The mutant fruits showed a retarded and less efficient suberization response at the stem scar wound, whereas the rate and strength of this response were positively correlated with ABA content. These results clearly indicate in vivo the involvement of ABA in the suberization-based wound-healing processes at the stem scar tissue of tomato fruits.

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Coordinate expression of AOS genes and JA accumulation: JA is not required for initiation of closing layer in wound healing tubers

Cited by 31 publications

References 33 publications

The potato suberin feruloyl transferase FHT which accumulates in the phellogen is induced by wounding and regulated by abscisic and salicylic acids

The potato suberin feruloyl transferase FHT which accumulates in the phellogen is induced by wounding and regulated by abscisic and salicylic acids

Differential induction of polar and non‐polar metabolism during wound‐induced suberization in potato (Solanum tuberosum L.) tubers

Abscisic acid mediates the formation of a suberized stem scar tissue in tomato fruits

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