Genotoxicity study of nano Al2O3, TiO2 and ZnO along with UV-B exposure: An Allium cepa root tip assay

Debnath, Pampa; Mondal, Naba Kumar; Sen, Kamalesh; Mishra, Debojyoti

doi:10.1016/j.scitotenv.2020.136592

Cited by 40 publications

(15 citation statements)

References 64 publications

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“…1e). Debnath et al (2020) stated that cellular DNA is a conspicuous target for UV-dependent genetic impairment in plants, and stickiness may be a result of polymeric deterioration of chromosomal DNA. Likewise the above-mentioned CAs, the increase in the amount of lycopene applied simultaneously with UV-C resulted in a gradual decrease in sticky chromosome frequency in G5 and G6.…”

Section: Resultsmentioning

confidence: 99%

Extenuating role of lycopene against 254-nm UV-C radiation-mediated damages in Allium cepa L. roots

Çavuşoğlu

Macar

et al. 2021

Environ Sci Pollut Res

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Section: Resultsmentioning

confidence: 99%

Extenuating role of lycopene against 254-nm UV-C radiation-mediated damages in Allium cepa L. roots

Çavuşoğlu

Macar

et al. 2021

Environ Sci Pollut Res

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“…Genotoxicity of ZnO NPs and also TiO 2 NPs were confirmed for Allium cepa root tips. Anaphase with multiple chromatin bridges and lagged metaphase were evidence of ZnO NPs and TiO 2 NPs at concentrations as low as 0.1 mg/L [ 127 ]. Genotoxic effects of ZnO NPs on root cells of Allium cepa and Vicia faba were reported by Ghosh et al [ 119 ].…”

Section: Discussionmentioning

confidence: 99%

Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death

2020

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Nanomaterials, including zinc oxide nanoparticles (ZnO NPs), have a great application potential in many fields, such as medicine, the textile industry, electronics, and cosmetics. Their impact on the environment must be carefully investigated and specified due to their wide range of application. However, the amount of data on possible negative effects of ZnO NPs on plants at the cellular level are still insufficient. Thus, we focused on the effect of ZnO NPs on tobacco BY-2 cells, i.e., a widely accepted plant cell model. Adverse effects of ZnO NPs on both growth and biochemical parameters were observed. In addition, reactive oxygen and nitrogen species visualizations confirmed that ZnO NPs may induce oxidative stress. All these changes were associated with the lipid peroxidation and changes in the plasma membrane integrity, which together with endoplasmatic reticulum and mitochondrial dysfunction led to autophagy and programmed cell death. The present study demonstrates that the phytotoxic effect of ZnO NPs on the BY-2 cells is very complex and needs further investigation.

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“…Additionally, the fish are frequently at the top of the food chain in aquatic systems and they feed on their prey, which bioaccumulates a substantial amount of metals (Rashed, 2001; Olaifa et al, 2004). They are important points at which concentrations of metals will become toxic and how these levels will affect the fish (Debnath et al, 2020). Several researchers also studied the toxic effects of individual and complex metal mixtures on fish such as Prochilodus lineatus , Labeo rohita , Cirrhina mrigala , Catla catla , Ctenopharyngodon idella , Cyprinus carpio , and Danio rerio (Galindo et al, 2010; Kousar and Javed, 2015; García-Medina et al, 2017; Gökalp et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

In vivo assessment of genotoxic effects in Cyprinus carpio L., 1758 (Teleostei: Cyprinidae) exposed to selected metal(oid)s

Kontaş

2022

Toxicol Ind Health

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Heavy metals and metalloids originating from industrial, agricultural, and urban wastes and increasing in aquatic ecosystems cause genotoxic damage to fish species. This study aimed to determine the potential genotoxic effects of mixtures of aluminum, arsenic, and manganese in Cyprinus carpio. The effects of the mixtures on erythrocyte cells of C. carpio were examined using the comet assay, micronucleus test, and erythrocytic nuclear abnormalities in two groups after exposure to three doses of the mixtures (Group A; Dose 1: 0.3 + 0.1 + 0.02 mg/L, Dose 2: 0.6 + 0.2 + 0.04 mg/L, Dose 3: 0.9 + 0.3 + 0.06 mg/L and Group B; Dose 1: 1 + 3 +0.1 mg/L, Dose 2: 2 + 6 + 0.2 mg/L, Dose 3: 3 + 9 + 0.3 mg/L). Experimental groups were formed according to the permissible limits specified in the Turkish Surface Water Quality Regulation (TSWQR). The results of comet assay parameters such as tail DNA %, tail moment, and olive tail moment confirmed the genotoxic effect of metal(oid)s mixtures on erythrocyte cells compared with control groups and showed that DNA damage increased with increasing the concentrations. The micronucleus and other nuclear abnormalities such as blebbed nuclei, notched nuclei, eightshaped nuclei, lobed nuclei, and binucleated cells were detected in the erythrocyte cells exposed to the mixtures. Consequently, it was found that the frequency of micronucleus and erythrocytic nuclear abnormalities significantly increased in the erythrocyte cells exposed to metal(oid) concentrations compared to control groups. These results show the existence of potential genotoxicity in C. carpio even at the minimum values specified in the TSWQR after exposure to the mixtures.

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Genotoxicity study of nano Al2O3, TiO2 and ZnO along with UV-B exposure: An Allium cepa root tip assay

Cited by 40 publications

References 64 publications

Extenuating role of lycopene against 254-nm UV-C radiation-mediated damages in Allium cepa L. roots

Extenuating role of lycopene against 254-nm UV-C radiation-mediated damages in Allium cepa L. roots

Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death

In vivo assessment of genotoxic effects in Cyprinus carpio L., 1758 (Teleostei: Cyprinidae) exposed to selected metal(oid)s

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