Genotoxicity of water samples from Dianchi lake detected by the Vicia faba micronucleus test

Duan, Changqun; Hu, Bin; Jiang, Xiaohua; Wen, Chuanhao; Wang, Zhenghong; Wang, Yingxue

doi:10.1016/s0027-5107(99)00053-6

Cited by 18 publications

(5 citation statements)

References 9 publications

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“…Micronuclei are chromosome fragments or whole chromosomes that lag at cell division due to the lack of a centromere, damage, or a defect in cytokinesis [Heddle et al, 1991]. Genotoxicity has been measured in the marine and freshwater environments by scoring MN induction in plants [Duan et al, 1999;Yang, 1999;Monarca et al, 2003], amphibians [Fernandez et al, 1989;Djomo et al, 2000;Wirz et al, 2005], mussels [Klobucar et al, 2003;Barsiene et al, 2004;Nigro et al, 2006], and fish [Ayllon and Garcia-Vazquez, 2000;De Andrade et al, 2004;Cavas and Ergene-Gozukara, 2005b;Porto et al, 2005;Da Silva Souza and Fontanetti, 2006]. Fish are extremely valuable in toxicity monitoring as they accumulate hazardous compounds in their tissues, are directly or indirectly consumed by humans, and are capable of transforming xenobiotic compounds into carcinogenic and mutagenic metabolites [Law, 2001;Van Der Oost et al, 2003].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of river water genotoxicity using the piscine micronucleus test

Ergene

Çavaş

Çelık

et al. 2007

Environ and Mol Mutagen

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The Berdan River, which empties into the Mediterranean Sea on the east coast of Turkey, receives discharges of industrial and municipal waste. In the present study, the in vivo piscine micronucleus (MN) test was used to evaluate the genotoxicity of water samples collected from different locations along the Berdan River. Nile tilapia (Oreochromis niloticus) were exposed in the laboratory for 2, 4, and 6 days, and micronuclei were evaluated in peripheral blood erythrocytes, gill cells, and caudal fin epithelial cells. A single dose of 5 mg/L cyclophosphamide was used as a positive control. In addition to micronuclei, nuclear abnormalities (NAs), such as binucleated cells and blebbed, notched, and lobed nuclei, were assessed in the erythrocytes, and chemical analyses were carried out to determine the amount of heavy metals in the water samples. MN and NA frequencies were significantly elevated (up to 2- to 3-fold) in fish exposed to river water samples taken downstream of potential discharges, and the elevated responses in gill and fin cells were related to the concentration of heavy metals in the water. MN frequencies (expressed as micronucleated cells/1,000 cells), in both treated and untreated fish, were greatest in gill cells (range: 0.80-3.70), and generally lower in erythrocytes (range: 0.50-2.80), and fin cells (range: 0.45-1.70). The results of this study indicate that the Berdan River is contaminated with genotoxic pollutants and that the genotoxicity is related to the discharge of wastes into the river water.

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Section: Introductionmentioning

confidence: 99%

Evaluation of river water genotoxicity using the piscine micronucleus test

Ergene

Çavaş

Çelık

et al. 2007

Environ and Mol Mutagen

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“…The difference in toxicity observed between RS and DS at the 50% dose could only be due to the OM content, since the concentrations of Cu and Zn were similar. Phenols in the raw slurry that had been shown to be toxic or genotoxic [10][11][12][13][14][15][16][17][18][19][20][21][22][23] were degraded during anaerobic digestion [27,28], which may explain the decrease of the toxic effect of the OM after treatment.…”

Section: Micronucleus Test Of Slurry Leachatesmentioning

confidence: 99%

“…Plant assays are highly sensitive, quite easy to conduct, inexpensive, and good predictors of carcinogenicity [12]. Particularly, the Vicia faba micronucleus test has been shown to be sensitive in evaluating chromosomal aberrations and assessing genotoxicity from both organic and inorganic contaminants of soil [13,14], sediment [15], organic material such as sewage sludge or composts [16], and water [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the genotoxicity of Cu and Zn in raw and anaerobically digested slurry with the Vicia faba micronucleus test

Marcato-Romain

Pinelli

Pourrut

et al. 2009

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Vicia faba and Allium cepa are widely used for the evaluation of genotoxic potential of disinfected wastewater, surface water, and drinking water [12,16,[34][35][36][37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Influence of Temperature on Mutagenicity in Plants Exposed to Surface Disinfected Drinking Water

Gustavino¹,

Ceretti²,

Zani³

et al. 2012

JWARP

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Disinfection of surface drinking water, particularly water chlorination, produces by-products with potential genotoxic and/or carcinogenic activity. A study carried out at a pilot plant for drinking water disinfection of lake water revealed mutagenic activity of three different disinfectants (sodium hypochlorite, chlorine dioxide and peracetic acid) in different seasons using in situ mutagenicity assays, both in animal (micronucleus test) and in plant organisms (anaphase chromosomal aberration and micronucleus tests). The effects of the disinfectants appeared to be modulated by the season of exposure. In this study, we tried to understand if (and to what extent) the temperature parameter could actually play an independent role in the registered seasonal variation of mutagenic effects, neglecting the variation of other parameters, e.g. physical conditions and chemical composition of the lake water. Therefore plants (Allium cepa for chromosomal aberration test and Vicia faba for micronucleus test) were exposed to the same disinfected lake-water samples at different temperatures (10℃, 20℃ and 30℃), according the ones registered during the in situ experiment. Long-term expo- sure at the temperatures of 20℃ (both Vicia faba and Allium cepa) and 30℃ (Vicia faba only) to disinfected waters induced clear mutagenic effects. These results show that temperature is an important variable which should be taken into account when in situ exposure of plants is planned for mutagenicity testing. Also, different plant systems clearly show specific temperature ranges suitable for their growth, thereby indicating the need for an accurate selection of the test organism for a specific experimental plan

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Genotoxicity of water samples from Dianchi lake detected by the Vicia faba micronucleus test

Cited by 18 publications

References 9 publications

Evaluation of river water genotoxicity using the piscine micronucleus test

Evaluation of river water genotoxicity using the piscine micronucleus test

Assessment of the genotoxicity of Cu and Zn in raw and anaerobically digested slurry with the Vicia faba micronucleus test

Influence of Temperature on Mutagenicity in Plants Exposed to Surface Disinfected Drinking Water

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