Assessment of the genotoxicity of Cu and Zn in raw and anaerobically digested slurry with the Vicia faba micronucleus test

Marcato-Romain, Claire-Emmanuelle; Pinelli, Éric; Pourrut, Bertrand; Silvestre, Jérôme; Guiresse, Maritxu

doi:10.1016/j.mrgentox.2008.10.018

Cited by 41 publications

(24 citation statements)

References 34 publications

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“…Exposure time was 30 h (6 h for the treated groups followed by a 24 h recovery period). In view of lead toxicity after 24 h of exposure (blackening of the root tips and loss of mitosis), a 24 h recovery period was enough to develop micronuclei (AFNOR, 2004;El Hajjouji et al, 2007;Marcato-Romain et al, 2009). Maleic hydrazide (MH, Sigma Chemical Co., St. Louis, MO, USA) at the concentration of 40 mM was used as the positive control and aerated Hoagland's solution alone was used as the negative control (Table 1).…”

Section: Faba Micronucleus Testmentioning

confidence: 99%

“…Herná ndez -Allica et al (2007) also reported that proper management of the EDTA concentration can increase the uptake of metals with low phytoavailability like Pb and reduce their phytotoxicity. Recently, Marcato-Romain et al (2009) presented that MN frequencies in V. faba root tips were more than ten-fold higher in hydroponic exposure compared to direct contact in soil under the same range of pollutant concentrations. These observations could be related to lead speciation (different between soil and solution), with a particular protecting effect of soil organic matter.…”

Section: Relationship Between Lead Uptake and Genotoxicity In The Prementioning

confidence: 99%

“…Plant assays are quite easy to conduct, inexpensive, rapid and good predictors of genotoxicity (Panda and Panda, 2002). In particular, the V. faba micronucleus test is a very sensitive and useful method for the detection of both clastogenic and aneugenic effects (Duan et al, 2000;El Hajjouji et al, 2007;Marcato-Romain et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Lead-induced genotoxicity to V. faba was studied using current contact with aqueous extract or direct contact with soil by Marcato-Romain et al (2009). Due to lower genotoxicity observed in the case of direct contact with soil, these authors concluded to a potential influence of soil organic matters.…”

Section: Introductionmentioning

confidence: 99%

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Lead-induced genotoxicity to Vicia faba L. roots in relation with metal cell uptake and initial speciation

Shahid

Pinelli

Pourrut

et al. 2011

Ecotoxicology and Environmental Safety

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228

102

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. Lead-induced genotoxicity to Vicia faba L.roots in relation with metal cell uptake and initial speciation. Ecotoxicology and Environmental Safety, Elsevier, 2011, vol. 74, pp. 78-84. <10.1016/j.ecoenv.2010 a b s t r a c tFormation of organometallic complexes in soil solution strongly influence metals phytoavailability. However, only few studies deal with the influence of metal speciation both on plant uptake and genotoxicity. In the present study, Vicia faba seedlings were exposed for 6 h in controlled hydroponic conditions to 5 mM of lead nitrate alone and chelated to varying degrees by different organic ligands.Ethylenediaminetetraacetic acid and citric acid were, respectively, chosen as models of humic substances and low weight organic acids present in natural soil solutions. Visual Minteq software was used to estimate free lead cations concentration and ultimately to design the experimental layout. For all experimental conditions, both micronucleus test and measure of lead uptake by plants were finally performed. Chelation of Pb by EDTA, a strong chelator, dose-dependently increased the uptake in V. faba roots while its genotoxicity was significantly reduced, suggesting a protective role of EDTA. A weak correlation was observed between total lead concentration absorbed by roots and genotoxicity (r 2 ¼0.65). In contrast, a strong relationship (r 2 ¼0.93) exists between Pb 2 + concentration in exposure media and genotoxicity in the experiment performed with EDTA. Citric acid induced labile organometallic complexes did not demonstrate any significant changes in lead genotoxicity or uptake. These results demonstrate that metal speciation knowledge could improve the interpretation of V. faba genotoxicity test performed to test soil quality.

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Section: Faba Micronucleus Testmentioning

confidence: 99%

Section: Relationship Between Lead Uptake and Genotoxicity In The Prementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lead-induced genotoxicity to Vicia faba L. roots in relation with metal cell uptake and initial speciation

Shahid

Pinelli

Pourrut

et al. 2011

Ecotoxicology and Environmental Safety

Self Cite

228

102

View full text Add to dashboard Cite

show abstract

“…Contrasting and inconclusive data on the genotoxicity of Zn compounds are available in literature. The observations recorded on several herbaceous and woody species showed differentiated responses, depending on the concentration range, exposure duration, plant species, class of Zn compounds, and treatment with unary or binary solutions (Ince et al, 1999;Marcato-Romain et al, 2009;Patra et al, 2004;Steinkellner et al, 1998), but also on the number of somatic and metacentric chromosomes or on the length of the diploid complement (Ma et al, 1995). Some reports state that high Zn concentrations are not strongly genotoxic (Codina et al, 2000;Gómez-Arroyo et al 2001;Marcato-Romain et al, 2009).…”

Section: Frequency Of Abnormal Metaphasesmentioning

confidence: 99%

Zinc-Induced Genotoxic Effects in Root Meristems of Barley Seedlings

Truta

Gherghel

Bara

et al. 2013

Not Bot Hort Agrobot Cluj

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The pollution increase, as a result of the release into environment of genotoxic chemicals, including heavy metals, largely affects the ecosystems and the health of living organisms. Although zinc is not considered highly phytotoxic, its excess becomes noxious. In literature, the reports on zinc genotoxicity are equivocal. Therefore, the objective of this work was to evaluate the amplitude of cytogenetic damage induced in Hordeum vulgare L. cv. 'Madalin' after seed treatment with different concentrations (10, 100, 250, 500 µM) of Zn +2 , provided as zinc sulphate and zinc acetate. The mitostimulatory effect was present at all concentrations of both zinc compounds. The rate of ana-telophase aberrations exceeded by 2 -3 times the control, and the frequency of metaphase disturbances was 5.0-10.0 times higher than the control. The results indicate the clastogenic and aneugenic potential of zinc in barley and constitute a signal about the risks of its increasing presence into the environment, with repercussions on living systems, even on human health, due to the extensive use of zinc compounds including as pesticides.

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Nutrients and Plant Hormones in Anaerobic Digestates

Dong

2020

Biorefinery of Inorganics

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Assessment of the genotoxicity of Cu and Zn in raw and anaerobically digested slurry with the Vicia faba micronucleus test

Cited by 41 publications

References 34 publications

Lead-induced genotoxicity to Vicia faba L. roots in relation with metal cell uptake and initial speciation

Lead-induced genotoxicity to Vicia faba L. roots in relation with metal cell uptake and initial speciation

Zinc-Induced Genotoxic Effects in Root Meristems of Barley Seedlings

Nutrients and Plant Hormones in Anaerobic Digestates

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