Genotoxicity of quercetin in the micronucleus assay in mouse bone marrow erythrocytes, human lymphocytes, V79 cell line and identification of kinetochore-containing (CREST staining) micronuclei in human lymphocytes

Caria, Helena; Chaveca, Teresa; Laires, A.; Rueff, José

doi:10.1016/0165-1218(95)90075-6

Cited by 82 publications

(27 citation statements)

References 31 publications

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“…The latter findings were originally correlated with the possible clastogenic action of the bracken fern compounds. Quercetin, the most probable candidate, has been described as mutagenic (Walter-Moura et al, 1988) and clastogenic (Caria et al, 1995 ;Carver et al, 1983 ;Popp & Schimmer, 1991). The present data show that chromosomal damage occurs in the absence of bracken fern carcinogens and precedes or occurs independently of the development of haematuric disease and is probably due to BPV and its effect on chromosomal stability.…”

Section: Discussionsupporting

confidence: 49%

“…The objective of the present study was to verify the possibility of virus transmission through blood and its clastogenic activity in cows not exposed to a bracken fern diet. The clastogenic effect of bracken fern compounds has been assessed in vitro and in vivo (Caria et al, 1995 ;Carver et al, 1983) ; however, the potential contribution of BPV to clastogenicity has not been clarified. In this report, papillomavirus infection was achieved by intramuscular injection of blood samples taken from haematuric cows, since peripheral blood lymphocytes have been reported to harbour BPV (Campo et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

“…The virus is believed to interact with bracken fern compounds such as quercetin (5,7,3h,4h-tetrahydroxyflavone) in the malignant transformation of primary bovine cells in vitro (Pennie & Campo, 1992). Quercetin alone induces mutations in bacteria (Walter-Moura et al, 1997), has clastogenic effects in cultured mammalian cells (Caria et al, 1995 ;Carver et al, 1983), and, in eukaryotic cells, intercalates into DNA, causing single-strand breaks as a consequence of free radical generation (Popp & Schimmer, 1991). However, quercetin failed to induce cancer in controlled in vivo experiments (Zhu et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Bovine papillomavirus transmission and chromosomal aberrations: an experimental model.

Stocco

Lindsey

Ferraz

et al. 1998

Journal of General Virology

106

141

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Enzootic haematuria and urinary bladder cancer in cattle are associated with feeding on bracken fern and bovine papillomavirus (BPV) infection. An increased rate of chromosomal aberrations in peripheral blood lymphocytes from chronically affected haematuric cows raised in bracken fern pastures has been reported, suggesting the presence of BPV in the peripheral blood of afflicted animals. The purpose of the present investigation was to examine the role of peripheral blood as a potential BPVtransmitting agent and search for clastogenic effects in experimentally infected animals kept on a bracken fern-free diet. Healthy cows were inoculated with blood samples of haematuric animals every two weeks for 18 months. Recipient cows, their offspring, donor animals and a control group were kept on a bracken fern-free diet throughout the experiment. Clinical and molecular analyses for

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Section: Discussionsupporting

confidence: 49%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bovine papillomavirus transmission and chromosomal aberrations: an experimental model.

Stocco

Lindsey

Ferraz

et al. 1998

Journal of General Virology

106

141

View full text Add to dashboard Cite

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“…To determine the total number of MN in binucleated cells, a total of 1000 binucleated cells with well-preserved cytoplasm (500 per replicate) were scored for each subject. MN were scored blindly by the same reader and identified according to the criteria of Caria et al (1995) using 500-fold magnification.…”

Section: Methodsmentioning

confidence: 99%

Micronucleus analysis in a Portuguese population exposed to pesticides: Preliminary survey

Costa

Silva

Coelho

et al. 2007

International Journal of Hygiene and Environmental Health

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The general population is exposed in their everyday life to different chemicals namely to pesticides. Many of these compounds are capable of inducing mutations in DNA and lead to several diseases including cancer. With this study we intended to evaluate DNA damage inflicted by pesticide exposure in a population occupationally exposed to those chemicals by means of the micronucleus (MN) test. The obtained results showed a significant increase in MN frequency in occupationally exposed individuals (po0.001) compared with the control group. Higher frequencies of MN were associated with a specific workplace (greenhouses) and the lack of protective measures (gloves) during labour activities. These results reinforce that conditions in workplace should be improved to minimize exposure to these chemicals. This study also emphasizes the need to reinforce the good practices campaigns in order to enlighten those who work with pesticides on the potential hazard of occupational exposure and the importance of using protective measures.

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“…Moreover, In Escherichia coli, quercetin has been shown to induce SOS activity, reverse mutations, and DNA single strand breaks with or without metabolic activation [46]- [49]. These positive mutagenic and genotoxic responses in bacterial test systems have been confirmed in eukaryotic cells, including yeast [50] and mammalian cells included mouse, hamster, rat and human [51]- [55], in which sister chromatid exchanges, chromosomal aberrations, unscheduled DNA synthesis (UDS), and micronucleus formation are among some of the endpoints evaluated. However, in a study for a 2-year rodent carcinogenicity bioassay conducted by the National Toxicology Program (NTP), these genotoxic effects have not confirmed suggesting the safety of quercetin for oral consumption in humans [52] [56] [57].…”

Section: Introductionmentioning

confidence: 99%

Possible Synergistic Effect and Antioxidant Properties of Chitosan Nanoparticles and Quercetin against Carbon Tetrachloride-Induce Hepatotoxicity in Rats

El-Denshary¹,

Aljawish²,

El‐Nekeety³

et al. 2015

SNL

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This study was conducted to prepare chitosan nanoparticles (CNPs), to determine their properties and to evaluate the synergistic protective role of CNPs alone or in combination with quercetin (Q) against oxidative stress and hepatotoxicity in rats. Female Sprague-Dawley rats were divided into 12 groups (7 rats/group) and were maintained on their respective diet for 3 weeks as follow: control group, the group treated with CCl4 (100 mg/kg b.w twice a week); the groups received CNPs at low and high doses (140 and 280 mg/kg b.w); the group received Q (50 mg/kg b.w); the groups received CNPs at the low or high doses plus Q and the groups treated with CCl4 plus Q and/or CNPs at the two tested doses. Blood and liver samples were collected at the end of experiment period for biochemical and histological studies. The results indicated that chitosan showed deacetylation degree of 17.5% and 19.2% and the molar mass average of monomer was 168.35 g/mol and 169.1 g/mol by UV and IR methods respectively. The particle size of CNPs was around 100 nm with a rough surface. The in vivo results revealed that CCl4 induced biochemical and histological changes typical to those reported in the literature. Animals treated with CNPs at the two tested doses alone or in combination with Q were comparable to the control. CNPs alone or plus Q succeeded to induce significant improvements in the biochemical parameters and histological picture of the liver in rats treated with CCl4. This improvement was in dose-dependent manner for CNPs and was * Corresponding author. E. S. El-Denshary et al. 37more pronounced in the group treated with the high dose plus Q. It could be concluded that both CNPs and Q could induce protection against hepatotoxicity. Consequently, CNPs was a promise candidate as drug delivery in liver diseases treatments.

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Genotoxicity of quercetin in the micronucleus assay in mouse bone marrow erythrocytes, human lymphocytes, V79 cell line and identification of kinetochore-containing (CREST staining) micronuclei in human lymphocytes

Cited by 82 publications

References 31 publications

Bovine papillomavirus transmission and chromosomal aberrations: an experimental model.

Bovine papillomavirus transmission and chromosomal aberrations: an experimental model.

Micronucleus analysis in a Portuguese population exposed to pesticides: Preliminary survey

Possible Synergistic Effect and Antioxidant Properties of Chitosan Nanoparticles and Quercetin against Carbon Tetrachloride-Induce Hepatotoxicity in Rats

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