Bovine papillomavirus transmission and chromosomal aberrations: an experimental model.

Stocco, Rita de Cássia; Lindsey, Charles Julian; Ferraz, O. P.; Pinto, José Ricardo; Mirandola, R S; Benesi, Fernando José; Birgel, Eduardo Harry; Pereira, Carlos Alberto de Bragança; Be√ßak, W

doi:10.1099/0022-1317-79-9-2127

Cited by 103 publications

(153 citation statements)

References 22 publications

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“…BPV DNA can occur in circulating lymphocytes of cattle, and latent BPV infection of lymphocytes was induced in experimental cattle (Stocco dos Santos et al, 1998). We have previously described the presence of BPV DNA in different tissues, not only epithelia, with evidence of virus activity (Yaguiu et al, 2006(Yaguiu et al, , 2008.…”

Section: Discussionmentioning

confidence: 99%

“…However, the presence of viral DNA sequences in different cells such as those of peripheral blood and gametes has been reported (Stocco dos Santos et al, 1998;Carvalho et al, 2003;Freitas et al, 2003). The significance of the presence of these nucleic acid sequences in these tissues has not yet been determined, considering that there is yet no evidence of expression of these nucleic acid sequences and of their potential to initiate infection.…”

Section: Introductionmentioning

confidence: 97%

“…Possible evidence of effects of these nucleic acid sequences can include chromo-somal abnormalities found in peripheral blood lymphocytes from chronically infected animals (Walter-Moura et al, 1988) or animals experimentally exposed to the virus (Stocco dos Santos et al, 1998) or in a cell line obtained from bovine palate fibroblasts submitted to a specific protocol of transfection with active Ha-ras, BPV-4 protein E7 and exposed to quercetin pulse (Leal et al, 2003). These detected chromosome aberrations can indicate a possible action of viral DNA sequences on the host chromatin and its eventual expression (Avanzo JL, Góes LGB, Dagli MLZ, Birgel EH Jr., et al, unpublished results).…”

Section: Introductionmentioning

confidence: 99%

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Can established cultured papilloma cells harbor bovine papillomavirus?

Campos¹,

Trindade²,

Ferraz³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. Papillomaviruses have been reported to be very difficult to grow in cell culture. Also, there are no descriptions of cell cultures from lesions of bovine cutaneous papillomatosis, with identification of different bovine papilloma virus (BPV) DNA sedifferent bovine papilloma virus (BPV) DNA sequences. In the present report, we describe primary cell cultures from samples of cutaneous lesions (warts). We investigated the simultaneous presence of different BPV DNA sequences, comparing the original lesion to different passages of the cell cultures and to peripheral blood. BPV 1, 2 and 4 DNA sequences were found in lesion samples, and respective cell cultures and peripheral blood, supporting our previous hypothesis of the possible activity of these sequences in different samples and now also showing how they can be maintained in different passages of cell cultures.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Can established cultured papilloma cells harbor bovine papillomavirus?

Campos¹,

Trindade²,

Ferraz³

et al. 2008

Genet. Mol. Res.

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“…Furthermore, both horizontal and vertical transmission of BPV-2 infection from peripheral blood of infected animals has been experimentally documented in healthy cattle (Stocco dos Santos et al, 1998). BPV-1/-2 E5 DNA has been detected in PBMCs of cattle suffering from urothelial tumours of the urinary bladder and horses affected by sarcoids (Brandt et al, 2008).…”

Section: Introductionmentioning

confidence: 98%

“…In these animals, tumours of the urinary bladder are common, and it is believed that BPV-2 and some immunosuppressive and/or carcinogenic chemicals of It has been suggested that blood cells can be a site of latent virus, as episomal BPV DNA has been detected in circulating lymphocytes (Campo et al, 1994). Furthermore, both horizontal and vertical transmission of BPV-2 infection from peripheral blood of infected animals has been experimentally documented in healthy cattle (Stocco dos Santos et al, 1998). BPV-1/-2 E5 DNA has been detected in PBMCs of cattle suffering from urothelial tumours of the urinary bladder and horses affected by sarcoids (Brandt et al, 2008).…”

mentioning

confidence: 98%

PBMCs are additional sites of productive infection of bovine papillomavirus type 2

Roperto

Comazzi

Ciusani

et al. 2011

Journal of General Virology

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Bovine papillomavirus type 2 (BPV-2) is an oncogenic virus infecting both epithelial and mesenchymal cells. Its life cycle, similar to other papillomaviruses (PVs), appears to be linked to epithelial differentiation. Human and bovine PVs have been known to reside in a latent, episomal form in PBMCs; therefore, it is believed that blood cells, like all mesenchymal cells, function as non-permissive carriers. Here, for the first time in veterinary and comparative medicine, the BPV-2 E5 oncoprotein and the major structural L1 capsid protein, known to be expressed only in productive infections, were shown to occur in defined subsets of PBMCs. E5 oncoprotein was detected in sorted T-and B-cells as well as in monocytes by flow cytometry and Western blot analysis. However, CD4 + and CD8 + lymphocytes appeared to be the main circulating targets of the virus, thus possibly representing the most important reservoir of active BPV-2 in blood. L1 protein was identified by flow cytometry in a population of blood cells recognized as lymphocytes by morphological scatter properties. Western blot analysis was performed on lysates obtained from the sorted subpopulations of PBMCs and detected L1 protein in CD4 + and CD8 + cells only. Thus, this study showed that CD4 + and CD8 + lymphocytes are permissive for BPV-2 and are new, hitherto unknown sites of productive PV infection. In light of these observations, the life cycle of PVs needs to be revisited to gain novel insights into the epidemiology of BPV infection and the pathogenesis of related diseases. INTRODUCTIONBovine papillomaviruses (BPVs) are a heterogeneous group of viruses responsible for tumours of the skin, genital and paragenital area, eye, upper gastrointestinal tract and urinary bladder (IARC, 2007). BPVs, like all other papillomaviruses (PVs), are usually strictly species specific. However, cases of cross-species infection are known to occur. BPV type 1 (BPV-1) and BPV-2, belonging to the genus Deltapapillomavirus (de Villiers et al., 2004), are responsible for infections in equids resulting in sarcoids (Chambers et al., 2003;Trenfield et al., 1985), as well as in bison and water buffaloes leading to warts (Literák et al., 2006;Silvestre et al., 2009). In addition, a variant of BPV-8, classified in the genus Epsilonpapillomavirus, also causes papillomas of the skin in bison (Tomita et al., 2007). More recently, it has been suggested that BPVs could be responsible for cutaneous sarcoids in cats and captive African lions (Munday & Knight, 2010;Orbell et al., 2010).BPV-1 and -2 are closely related serotypes (Shafti-Keramat et al., 2009) and can infect both epithelial and mesenchymal cells. Similar to other PVs, BPV-1/-2 replication and virion production are confined to the epithelial region of the lesions, whilst infection of mesenchymal cells appears to be non-productive (Campo, 2006;Shafti-Keramat et al., 2009).BPV-2 is known to play a central role in bladder carcinogenesis of adult cattle reared on pasturelands rich in bracken fern. In these animals, tumours of the u...

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Viral Diseases of Ferrets

Kiupel¹,

Perpiñán²

2014

Biology and Diseases of the Ferret

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Bovine papillomavirus transmission and chromosomal aberrations: an experimental model.

Cited by 103 publications

References 22 publications

Can established cultured papilloma cells harbor bovine papillomavirus?

Can established cultured papilloma cells harbor bovine papillomavirus?

PBMCs are additional sites of productive infection of bovine papillomavirus type 2

Viral Diseases of Ferrets

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