Genotoxicity effects of medicinal plants extracts against bacterial species, Mycoplasma hominis

Abstract

Aim: To assess the antimicrobial activity and genotoxicity of three medicinal plants used by Saudi Arabian people as traditional medicine against Mycoplasma hominis. Methodology: Different concentrations of Syzygium aromaticum (clove), Vachellia nilotica (acacia), and Thyme vulgaris (thyme) extracts were used as antimicrobial agents against M. hominis, and their lethal effects on Mycoplasma genome DNA were analyzed using repetitive element PCR(Rep-PCR). Results: The aqueous extracts of clove and Acacia at 3.125 mg ml-1 were found to be active antimicrobials against three tested Mycoplasm. Thyme extract exhibited antimicrobial activity at 12.5 mg ml-1. Moreover, this extract revealed potent lethal activities as growth turbidity decreased with increasing concentration or exposure time as compared to untreated Mycoplasma. The results of Rep-PCR clearly indicate that changes occured in the number of genetic bands in treated Mycoplasma at certain concentrations as compared to untreated Mycoplasma. Interpretation: These results indicate the possibility of using these extracts as a source of antibacterial compounds for treating infections caused by Mycoplasma. Key words: Antimicrobial activity, Genotoxicity, Mycoplasma hominis, Medicinal plants, S. aromaticum

Evaluation of Antibacterial Activity of Selected Edible Herbs Against Genital Mycoplasmas Among University Students in Enugu State, Nigeria

“…It was observed that methanol and ethanol extracts inhibited the test organisms more than the aqueous extracts. These results are in accordance with [5] who found antimicrobial activity of cloves active against Mycoplasma isolates. The result presented in tables 8 and 9 indicate that the MIC of ethanol and methanol extract of the plants was higher than the MIC of the aqueous extracts of the test plants, The MIC results ranged from 6.23mg/ml to 12.5mg/ml.…”

“…The result presented in tables 8 and 9 indicate that the MIC of ethanol and methanol extract of the plants was higher than the MIC of the aqueous extracts of the test plants, The MIC results ranged from 6.23mg/ml to 12.5mg/ml. lower MIC were observed in the studies of [5]. Table 7 revealed that synergestic actions of extract on isolates showed a higher inhibition zones than when applied singly.…”

“…The highest extract yield was obtained from methanol extraction of Curcuma longa. The presence of phytochemicals in plants is responsible for their antioxidant and antibacterial activity [5]. Table 3 shows a high occurrence of M. hominis in females at (81.61%) and a higher occurrence of Ureaplasma Spp in men at (88%).…”

“…There was variation in the pattern of antibiotic susceptiblity of isolates to other agents. Variation in pattern of antibiotic susceptibility has been observed in other studies [5,19]. The in-discriminate use of antibiotics [9], the adverse effects of some antibiotics on host such as hypersensitivity, immune -suppression, allergic reactions and even loss of hearing [10] and emergence of multiple drug resistance among pathogens are challenges in the health care system which necessitate the need for new antimicrobial substances.…”

Revealing Berberis aristata potential as an anti-viral agent to combat Paramyxoviridae infection

“…Medicinal herbs and shrubs have been utilized for ages to treat chronic infectious ailments such as those caused by highly mutating organisms belonging to genus Mycoplasma (Yassin al., 2021). To determine the antiviral activity of B. aristata extracts, standardization of HA units produced was 4 HA viral concentration.…”