Genotoxicity assessment of acute exposure of chlorpyrifos to freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis

Evaluation of genotoxic and oxidative stress response to dimethoate in freshwater fish Channa punctatus (Bloch)

“…The induction of DNA damage and micro nucleated erythrocytes following exposure to dimethoate indicates a potential mutagenic effect of dimethoate in fish. Some studies have previously reported on the genotoxic and mutagenic potential of organophosphate insecticide cholrprifos and monocorotofos in C.punctatus (Ali et al, 2008). It is well established that toxicants with oxidative stress have the potential to attack DNA resulting in clastogenic and molecular damages (Jha, 2008).…”

“…Dimethoate induced a significant increase in the frequencies of MN and DNA damage in erythrocytes in a concentration and time dependent manner. MN test as a genotoxic endpoint for clastogenic effects of pollutants has been widely used in aquatic organisms such as mussels (Mytilus galloprovincialis), bivalve mollusks (Crassotrea gigas), rana tadpoles (Rana perezi), rainbow trout(Oncorhynchus mykiss), salmoni form fish(Umbra pygmea), Prussian carp (Carassius auratus gibelio) and tilapias (Oreochromis mossambicus) (Vernier et al, 1997).The results obtained in the present study are in accordance with the finding of Ali et al (2008) for chlorpyrifos in fish erythrocytes. The DNA damage detected in this study could have originated from DNA single strand breaks, DNA double strand breaks, DNA adducts formation and DNA-DNA and DNA-protein cross links (Mitchelmore and Chipman, 1998), resulting from the interaction of pesticides or their metabolites with DNA (Fairbairn et al, 1995).…”

“…The alkaline single cell gel electrophoresis (SCGE)/comet assay was performed as a three-layer procedure (Singh et al, 1988) with slight modification (Ali et al, 2008). First, the slides were cleaned with 100% ethanol and flame dried.…”

Micronucleus assay in human lymphocytes after exposure to alloxydim sodium herbicide in vitro

“…Although there was no study with micronucleus assay in cyclohexanedione herbicides, MN test system was used by many investigators for other pesticide groups and they reported increases in MN formation (Zeljezic and Garaj-Vrhovac 2004;Yüzbasioglu et al 2006;Ergene et al 2007;Ali et al 2008;Revankar and Shyama 2009). These results support our findings.…”

Effects of insecticides on aquatic ecosystems in Bangladesh

“…For instance, the 96-h LC50 of juvenile common carp (Cyprinus carpio) ranged between 149 µg/L and 582 µg/L , which is 7 to 26 times higher than that of Banded Gourami larvae we reported. The higher LC50 of chlorpyrifos for adult spotted snakehead (Channa punctatus) than that of the larvae Banded Gourami was also reported by Ali et al (2008).…”