Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips

Matsumoto, Sílvia Tamie; Mantovani, Mário; Malaguttii, Mirtis Irene Ariza; Dias, Ana Lúcia; Fonseca, Inês Cristina de Batista; Marin-Morales, Maria Aparecida

doi:10.1590/s1415-47572006000100028

Cited by 297 publications

(163 citation statements)

References 37 publications

(39 reference statements)

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“…Scalon et al (2010) have assessed the genotoxicity of the water collected from three points in the Sinos River during four seasons, using the comet assay in the fish Hyphessobrycon luetkenii, having found that the frequency of cells with DNA damage was higher during spring at the sites located in the upper and middle sections. Differences between sampling sites or periods in a river could be related to differences in the sources of contamination (types of pollutants), the volume of water (alteration in the concentration of genotoxic agents) and/or the cumulative effect of different sources (Matsumoto et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil

et al. 2015

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Some water bodies in the Sinos River Basin (SRB) have been suffering the effects of pollution by residential, industrial and agroindustrial wastewater. The presence of cytotoxic and genotoxic compounds could compromise the water quality and the balance of these ecosystems. In this context, the research aimed to evaluate the genotoxicity and cytotoxicity of the water at four sites along the SRB (in the cities of Santo Antônio da Patrulha, Parobé, Campo Bom and Esteio), using bioassays in fish and cell culture. Samples of surface water were collected and evaluated in vitro using the Astyanax jacuhiensis fish species (micronucleus test and comet assay) and the Vero lineage of cells (comet assay and cytotoxicity tests, neutral red -NR and tetrazolium MTT). The micronucleus test in fish showed no significant differences between the sampling sites, and neither did the comet assay and the MTT and NR tests in Vero cells. The comet assay showed an increase in genetic damage in the fish exposed to water samples collected in the middle and lower sections of the basin (Parobé, Campo Bom and Esteio) when compared to the upper section of the basin (Santo Antônio da Patrulha). The results indicate contamination by genotoxic substances starting in the middle section of the SRB.Keywords: water quality, micronucleus test, comet assay, fish bioassays, cell culture. Avaliação da genotoxicidade e da citotoxicidade de amostras de água da Bacia do Rio dos Sinos, sul do Brasil ResumoAlguns corpos d'água da Bacia Hidrográfica do Rio dos Sinos (BHRS) vêm sofrendo os efeitos da poluição por efluentes domésticos, industriais e agroindustriais. A presença de compostos citotóxicos e genotóxicos pode comprometer a qualidade da água e o equilíbrio desses ecossistemas. Neste contexto, o objetivo do trabalho foi avaliar a genotoxicidade e a citotoxicidade da água em quatro pontos ao longo da BHRS (Santo Antônio da Patrulha, Parobé, Campo Bom e Esteio), utilizando bioensaios em peixes e em cultura celular. As amostras de água de superfície foram coletadas e avaliadas in vitro utilizando a espécie de peixe Astyanax jacuhiensis (teste de micronúcleo e ensaio cometa) e a linhagem celular tipo Vero (ensaio cometa e os testes de citotoxicidade vermelho neutro -VN e tetrazólio MTT). O teste de micronúcleos em peixes não apresentou diferenças significativas entre os pontos de coleta, assim como o ensaio cometa e os testes VN e MTT nas células Vero. O ensaio cometa demonstrou aumento nos danos genéticos em peixes expostos às amostras de água coletadas nos trechos médio e inferior da bacia (Parobé, Campo Bom e Esteio) em relação ao trecho superior da bacia (Santo Antônio da Patrulha). Os resultados indicam contaminação por substâncias genotóxicas a partir do trecho médio da BHRS.Palavras-chave: qualidade da água, teste de micronúcleo, ensaio cometa, bioensaios com peixes, cultura celular.

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Section: Discussionmentioning

confidence: 99%

Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil

et al. 2015

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“…One could suggest that a synergistic effect of the elements found in vinasse could cause the observed changes. Also, according to Lerda [20] and Matsumoto et al [21], this can also be due to the exposure of cells to cadmium, copper, lead, chrome, and nickel, which increase the clastogenic and aneugenic effect on certain plants. The chemical analysis of vinasse samples did not reveal large quantities of these elements.…”

Section: Yamamoto and Kikuchimentioning

confidence: 99%

Mutagenic Action of Sugarcane Vinasse in the Tradescantia Pallida Test System

Pedro-Escher¹,

Maziviero²,

Fontanetti³

2014

J Ecosyst Ecogr

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The soil is a very complex and dynamic system and its contamination has become one of the main environmental concerns. Sugar-ethanol mills, for example, have a high hazard potential, mainly soil contamination, due to the production of different residues. The use of vinasse, a by-product of ethanol production, as fertilizer is a technically and economically feasible alternative. However, excessive quantities have been used, causing adverse changes in the soil. The present study was aimed at evaluating the toxic potential of vinasse in the Tradescantia pallida test system using the micronucleus test (Trad-MCN). Ten plants were exposed to ultrapure water as the negative control (NC), in MMS as the positive control (PC), raw vinasse (RV), and vinasse diluted to 50% (C1), vinasse diluted to 25% (C2), vinasse diluted to 12.5% (C3). The medium buds of each plant were used to assess the mutagenic effects based on the presence of micronuclei in tetrads. The results revealed that RV and C1 had a mutagenic potential compared to that of NC. The alterations observed suggest damage to the DNA of the test organism, which may not be reintegrated to the cell nucleus. The findings suggest caution in the application of this product to the soil despite this being an economically viable practice.

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“…16,[32][33][34][35] Fish have received special attention because they are efficient bioindicators of environmental quality and are capable of metabolizing xenobiotics and pollutants accumulated in the aquatic environment. [36][37][38][39] Additionally, they play different roles in the food chain and bioaccumulate contaminants dissolved in water. 40,41 However, few fish species manage to survive in both polluted urban streams and the different types of Amazonian waters, which are classified as white, clear, and black waters, each with different physical and chemical characteristics.…”

Section: Introductionmentioning

confidence: 99%

Genomic Organization Under Different Environmental Conditions:Hoplosternum Littoraleas a Model

et al. 2016

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The Amazon has abundant rivers, streams, and floodplains in both polluted and nonpolluted environments, which show great adaptability. Thus, the goal of this study was to map repetitive DNA sequences in both mitotic chromosomes and erythrocyte micronuclei of tamoatás from polluted and nonpolluted environments and to assess the possible genotoxic effects of these environments. Individuals were collected in Manaus, Amazonas (AM), and submitted to classical and molecular cytogenetic techniques, as well as to a blood micronucleus test. Diploid number equal to 60 chromosomes are present in all individuals, with 18S ribosomal DNA sites present in one chromosome pair and no interstitial telomeric sites on chromosomes. The micronucleus test showed no significant differences in pairwise comparisons between environments or collection sites, but the Rex3 retroelement was dispersed on the chromosomes of individuals from unpolluted environments and compartmentalized in individuals from polluted environments. Divergent numbers of 5S rDNA sites are present in individuals from unpolluted and polluted environments. The mapping of repetitive sequences revealed that micronuclei have different compositions both intra-and interindividually that suggests different regions are lost in the formation of micronuclei, and no single fragile region undergoes breaks, although repetitive DNA elements are involved in this process.

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Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips

Cited by 297 publications

References 37 publications

Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil

Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil

Mutagenic Action of Sugarcane Vinasse in the Tradescantia Pallida Test System

Genomic Organization Under Different Environmental Conditions:Hoplosternum Littoraleas a Model

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