Genotoxicity and cytotoxicity of mineral trioxide aggregate and regular and white Portland cements on Chinese hamster ovary (CHO) cells in vitro

Ribeiro, Daniel Araki; Sugui, Marina Mariko; Matsumoto, Mariza Akemi; Duarte, Marco Antônio Húngaro; Marques, Mariângela Esther Alencar; Salvadori, Daisy Maria Fávero

doi:10.1016/j.tripleo.2005.02.080

Cited by 78 publications

(68 citation statements)

References 17 publications

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“…Therefore, and taking into account the lack of data currently available, the assessment of the potential genotoxicity of CEM is justified. In addition, it has been reported in previous studies that MTA has no cytotoxic 7,31,32) and no genotoxic 15,20,33,34) effects in vitro. For these reasons, the aim of this study was to evaluate the biocompatibility of CEM compared with MTA.…”

Section: Discussionmentioning

confidence: 79%

Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells

et al. 2014

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Mineral trioxide aggregate (MTA) has shown good biocompatibility in several studies. In the present study, the genotoxic and cytotoxic effects of calcium enriched mixture (CEM) were evaluated compared with MTA using MTT and single-cell gel (comet) assays with serial ascending concentrations (0 to 1,000 µg/mL) of tested materials. Cytotoxicity data indicated that there is no significant difference between CEM and MTA at all concentrations except for the full concentration (1,000 µg/mL); CEM had lower cytotoxicity. Genotoxic effects were more evident with CEM at concentrations of 15.6 and 250 µg/mL; however, was less than that of MTA at concentrations of 500 and 1,000 µg/mL. The cytotoxicity and genotoxicity effects of the two experimental groups generally increased with consistency. Under the conditions of this study, CEM is biocompatible in terms of cyto-and genotoxicity. It appears to be an alternative to MTA as an endodontic biomaterial offering several advantages.

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Section: Discussionmentioning

confidence: 79%

Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells

et al. 2014

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“…It has already been demonstrated that the association of ZrO 2 with PC promotes radiopacity 10,11) , not causing damage to the fibroblasts 4) and periodontal ligament cells 12) . It was also showed that 30% of ZrO2 added to PC does not interfere in the hydration mechanism of this cement, providing calcium ions release and resulting in a bioactive material 14) .…”

Section: Discussionmentioning

confidence: 99%

“…MTA has proper physicochemical properties, sealing capacity, biocompatibility, and ability to induce bone mineralization [2][3][4] . MTA is basically composed by Portland cement (PC) which has physicochemical, antimicrobial and biological properties similar to commercially available MTA [3][4][5] . PC shows biocompatibility and high compressive strength allowing that this material may be suitable for medical indications, such as orthopedic applications 6) .…”

Section: Introductionmentioning

confidence: 99%

Radiopacity, pH and antimicrobial activity of Portland cement associated with micro- and nanoparticles of zirconium oxide and niobium oxide

et al. 2014

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The aim of this study was to evaluate some properties of the calcium silicate materials Mineral Trioxide Aggregate (MTA) and Portland cement (PC) with microparticulated (micro) and nanoparticulated (nano) zirconium oxide (ZrO 2 ) or niobium oxide (Nb 2 O 5 ). The experimental materials: White PC (PC), MTA-Angelus ® (MTA), PC+ZrO 2 micro, PC+ZrO 2 nano, PC+Nb 2 O 5 micro and PC+Nb 2 O 5 nano were submitted to radiopacity and pH evaluations. Furthermore, the antimicrobial activity against different microorganisms was assessed by agar diffusion test. MTA presented higher radiopacity than other materials. However, all materials except PC presented higher radiopacity than recommended by ISO/ADA. MTA promoted higher pH values in all analyzed periods (p≤0.05). At the initial periods, PC and PC+ZrO 2 micro showed pH similar to MTA. All materials showed antimicrobial activity against the evaluated microorganisms. In conclusion, ZrO 2 and Nb 2 O 5 could be alternative radiopacifiers to be added to calcium silicate materials.

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“…MTA showed no mutagenic or genotoxic effects according to results from the micronucleus test 25 and the comet assay 26 . MTA and Ca 3 SiO 5 cement differ in their specific chemical formulations.…”

Section: Discussionmentioning

confidence: 95%

Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement

et al. 2016

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Ca 3 SiO 5 is new cement based on the composition of Portland that has been developed to have superior physicochemical and biological properties. In a clinical evaluation, the cement did not appear to have cytotoxic properties and allowed for the proliferation of pulp cells and gingival fibroblasts. However, no previous studies have evaluated the genotoxicity or the mutagenicity of Ca 3 SiO 5 in vivo. Therefore, the goal of this study is to evaluate the genotoxic and mutagenic potential of Ca 3 SiO 5 -based cement in vivo. Twenty-four male Wistar rats were divided into 3 groups (n = 8). Group A rats received subcutaneous implantation of Ca 3 SiO 5 in the dorsum. Group B rats received a single dose of cyclophosphamide (positive control). Group C rats received subcutaneous implantation of empty tubes in the dorsum (negative control). After 24 hours, all animals were euthanized and the bone marrow of the femurs was collected for use in the comet assay and the micronucleus test. The comet assay revealed that the Ca 3 SiO 5 group had a tail intensity of 23.57 ± 7.70%, the cyclophosphamide group had a tail intensity of 27.43 ± 7.40%, and the negative control group had a tail intensity of 24.75 ± 5.55%. The average number of micronuclei was 6.25 (standard deviation, SD = 3.53) in the Ca 3 SiO 5 group, 9.75 (SD = 2.49) in the cyclophosphamide group, and 0.75 (SD = 1.03) in the negative control group. There was an increase in the micronuclei frequency in the Ca 3 SiO 5 group compared to that of the negative control group (p < 0.05). Our data showed that exposure to the Ca 3 SiO 5 -based cement resulted in an increase in the frequency of micronuclei, but no genotoxicity was detected according to the comet assay.

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Genotoxicity and cytotoxicity of mineral trioxide aggregate and regular and white Portland cements on Chinese hamster ovary (CHO) cells in vitro

Cited by 78 publications

References 17 publications

Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells

Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells

Radiopacity, pH and antimicrobial activity of Portland cement associated with micro- and nanoparticles of zirconium oxide and niobium oxide

Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement

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