Genotoxicity and cytotoxicity assay of water sampled from the underground nuclear explosion site in the north of the Perm region (Russia)

Evseeva, T.; Гераськин, С. А.; Шуктомова, И. И.; Taskaev, A. I.

doi:10.1016/j.jenvrad.2004.08.014

Cited by 32 publications

(16 citation statements)

References 13 publications

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“…Indeed, in both studies carried out in different radioecological conditions, the high levels of genotoxicity are observed in all samples tested, with the important contribution from severe types of cell damage. The present work and our previous studies [7][8][9] show that an adequate environment quality assessment cannot rely only on information about pollutants concentrations. Authorities in charge of controlling the levels of industrial load have dealt with limited lists of toxicants.…”

Section: Discussionsupporting

confidence: 52%

“…Authorities in charge of controlling the levels of industrial load have dealt with limited lists of toxicants. Furthermore, a mutual intensification of the effects from environmental contaminants is very possible, especially when they occur in situations of low-level exposures, as demonstrated earlier [8,9]. It is therefore impossible to estimate the biological risk from a combined exposure if based only on the knowledge of contaminants levels in the environment.…”

Section: Discussionmentioning

confidence: 99%

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Bioindication-based estimates as an integral part of the environment quality assessment

et al. 2009

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Abstract.A complex approach for the environment health assessment integrating information on contaminants levels registered in environment compartments and bioindication-based estimates is developed and applied to two studies carried out at sites with different scenarios of long-term impact. The environment state is assessed at the territory of a nuclear waste storage facility, Obninsk, Russia, and in the post-mining areas of the Upper Silesia, Poland with enhanced levels of natural radioactivity. Concentrations of micro-, macroelements and appropriate radionuclides activities are measured in samples of water and sediment. With the Allium cepa plant system, phyto-and genotoxicity of the sampled water and soil is estimated to assess a hazardous potential of the complex contamination. As biological endpoints, frequency and spectrum of chromosome aberrations and mitotic abnormalities in ana-telophase cells as well as mitotic activity in Allium cepa root tips are used. An approach and techniques are developed to reveal factors and contamination components that play a governing role in an induction of the observed biological effect and estimate their relative contribution. Compatibility of findings observed and legislative standards adopted are discussed. From the studies carried out, the Allium-test proved to be a sensitive and simple bioassay efficient in genotoxic monitoring of environmental pollutants.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Bioindication-based estimates as an integral part of the environment quality assessment

et al. 2009

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“…On the contrary, the observed effect at soil pollution by 137 Cs, Pb and pesticides averaged only 50% from anticipated one proceeding from the additive model. Furthermore, our data [5,6] on genotoxicity assay of water samples from the natural reservoirs formed both in the nuclear explosion epicenter and located near the radium production industry storage cell suggest that substantial biological effects may be caused by metal and radionuclide combined exposure at concentrations below permissible exposure limits for human due to synergic response. Therefore, an application of findings on a separate action to a prediction of combined exposure biological effects is unacceptable and causes essential deviations from experimentally observed data.…”

Section: Synergetic and Antagonistic Effects Of Different Factors Commentioning

confidence: 82%

Plants ecotoxicology. A case of low doses and multipollutant exposure

et al. 2005

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Abstract. Results of laboratory, "green-house" and long-term field experiments carried out on different plant species (spring barley, Scots pine, bulb onion and others) to study ecotoxical effects of low doses and concentrations of such common environmental factors as acute and chronic γ-radiation, heavy metals, pesticides, artificial and heavy natural radionuclides, are presented. Special attention is paid to ecotoxic effects of chronic low dose exposures, synergistic and antagonistic effects of different factors' combined action. The results of long-term field experiments in the 30-km Chernobyl NPP zone and in the vicinity of a radioactive wastes storage facility are discussed. The data presented suggest that the further evolution of investigations in this field would issue in the development of a theoretical bases and practical procedures for environmental protection against radioactivity, taking into account the new experimentally confirmed facts about the presence of such essentially important singularities as the nonlinearity of a dose-effect relationship, radiation-induced genomic instability, phenomenon of radioadaptation, increased probability of synergetic and antagonistic effects of the combined action of different nature factors.

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“…For this spectrum, the absence of severe (lethal) alterations in cell (multi-polar mitoses, -mitoses, changes in the nucleus form, chromosome adhesion) is reported. Such genetic alterations in cells are caused by a combined action of radionuclides and chemicals found in water in relatively small concentrations [4,7,8].…”

Section: Results and Conclusionmentioning

confidence: 99%

“…The onion testsystem was employed to evaluate soil toxicity from the Chernobyl affected region [3]. Results from biotesting of water bodies at the site of underground nuclear detonation in the northern part of the Perm region have demonstrated that combined action of chemical and radiation factors causes triggering of significant biological effects in the test-object [4]. The application of biotesting methods in conditions of combined radiation and chemical pollution of a territory is the basis for the development of new principles for radiation protection of biota [5].…”

Section: Introductionmentioning

confidence: 99%

Toxicity assessment of natural waters from the Obninsk radioactive waste repository

2009

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Abstract. The assessment of cyto-and genotoxicity of water samples from a regional radioactive waste repository using the Allium-test was carry out. Biotesting of natural waters from the site has demonstrated that negative biological effects are generated under the influence of water from well, located near the source of contamination. The percentage of aberrant cells in water samples from this well 4 is 2 times higher than in the control or other sources. Effects of water composition at the genome level are season independent. The Allium-test was shown to be highly sensitive and effective in testing a combined effect from radiation and chemical factors in field conditions.

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Genotoxicity and cytotoxicity assay of water sampled from the underground nuclear explosion site in the north of the Perm region (Russia)

Cited by 32 publications

References 13 publications

Bioindication-based estimates as an integral part of the environment quality assessment

Bioindication-based estimates as an integral part of the environment quality assessment

Plants ecotoxicology. A case of low doses and multipollutant exposure

Toxicity assessment of natural waters from the Obninsk radioactive waste repository

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