Genotoxicity and cytotoxicity analysis of curcumin and sunset yellow in human lymphocyte culture

Haverić, Anja; Haverić, Sanin; Hadžić, Maida; Lojo-Kadric, Naida; Ibrulj, Slavka

doi:10.14715/cmb/2018.64.3.14

Cited by 10 publications

(6 citation statements)

References 27 publications

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“…Apoptosis induction in tumor cells was the most efficient in curcumin concentrations of 20-25 µM and longer treatment periods while higher concentrations increased ratio of necrotic cells (Kuo et al, 1996;Syng-ai et al, 2004;Moustapha et al,2015). Genotoxic and cytotoxic effects of different curcumin concentrations (1, 2, 4, and 8 mM) in normal human lymphocytes culture were not recorded (Haverić et al, 2018b). However, curcumin citotoxicity and apoptosis induction has been also reported in some normal cells (Nelson et al, 2017).…”

Section: Resultsmentioning

confidence: 97%

Effects of Curcumin and Luteolin on Viability and Cell Death Induction in NFS-60 Cell Line

Haverić

Goletić

Durgut

et al. 2018

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Inducing cell death in tumor cells has been recognized as a promising strategy in curing tumors. Parallely, natural products, especially those with long-known usage in folk medicine, are gaining demanding and extensive clinical interests. Aiming to contribute to overall knowledge of curcumin and luteolin antitumour potentials, we analyzed their effects on cell death induction in NFS-60 cell line, using Trypan blue exclusion assay and TransDetect® Anenexin V-EGFP/PI assay. Results show that both tested agents induce cell death, especially in higher applied concentrations, but further investigations are needed to elucidate the mechanisms behind it.

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Section: Resultsmentioning

confidence: 97%

Effects of Curcumin and Luteolin on Viability and Cell Death Induction in NFS-60 Cell Line

Haverić

Goletić

Durgut

et al. 2018

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“…Human peripheral lymphocyte cultures were studied with chromosomal aberrations assay and micronucleus assay. As a result of this study, it was determined that sunset yellow were showed dosedependent genotoxic potential in both assays (Haverić et al, 2018). Due to the widespread use of stevia extracts, it was investigated in human peripheral blood lymphocytes.…”

Section: Some Of the Genotoxicity Studies With Food Additivesmentioning

confidence: 97%

Food Additives and Genotoxicity

Altunkaynak

Yilmaz

2021

International Journal of Science Letters

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In today's changing conditions, there has been an increase in the consumption of ready-made food with the change in eating habits. Moreover, parallel to the increase in ready-made food production, there has been an increase in the food additives used. The dose amounts of food additives are determined as a result of experimental analyses. However, some additives show long-term toxic effects on the human body in genotoxicity tests. In this review, definition of substances, purposes of usage, classification, genotoxicity, definitions of tests and publications of genotoxicity studies in food additives were discussed. The search was conducted in peer-reviewed journals using Science-Direct, Web of Science and Google Scholar. In this study, genotoxicity studies conducted with food additives between 2015-2021 were complied. For this purpose, the keywords ''food additive'', ''genotoxicity'' were used together and research articles were included in this study.

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“…In another micronucleus study conducted on human lymphocytes revealed the mutagenic action of azo FCA at 2500 µg mLˉ1 concentration (Sekeroglu et al, 2017). Likewise, the genotoxic and cytotoxic studies of turmeric yellow and sunset yellow FCA conducted on human lymphocyte culture showed significant structural and chromatid-associated aberrations at 4 and 8 mM concentrations, proving its hazardous effect on human health (Haverić et al, 2018). Chequer et al (2017) investigated the molecular impact and expression profile of cherry pink and tartrazine FCAs on human DNA repair genes and cell cycle.…”

Section: Microscopic Analysismentioning

confidence: 99%

Geno-Toxic Appraisal of Widely Used Food Color Additives on Model Plant Allium cepa Root Tip Cells

Farheen¹,

Mansoor²,

Abid³

2021

JIS

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Azo dyes are consisted of nitrogen and carbon atom having R-N= N-R' functional group in which Rs is usually aryl. The delocalization of aryl gives vivid colors such as red, orange, and yellow. It is usually produced by an electrophilic substitution reaction of an aryl diazotization with acetoacetic amide or aromatic amines (Clarke and Kirner, 1941), which are well documented carcinogenic and mutagenic agents (Khan et al., 2020).Several studies have been conducted on the nature and characterization of azo dyes which presented a higher level of cytotoxicity on plants cells, severe allergic reactions in humans, and even cancer in lab-tested animals than any other synthetic FCAs (Rangan and Barcelous, 2009;Micic et al., 2014;Kaur et al., Abstract | The frequent use of meal and beverages having food color additives (FCAs) may cause mutation in humans' proto-oncogenes which leads to carcinogenicity. The current findings aimed to evaluate the genotoxic impact of widely used azo FCAs on the cell cycle by using onion as a model plant. The study was designed in a complete randomized design where the grown onion roots were exposed to 0%, 0.001%, 0.01%, 0.1%, and 1% concentration of FCA for 120 hours for macroscopic and 36 h for microscopic evaluation. The onion root tips morpho-physiology was severely affected as the concentration of FCAs increased. The macroscopic analysis manifested that allura red had 95% broken-ended extremely thin and transparent root tips at 1% concentration. In the microscopic analysis, FCAs induced eight different kinds of chromosomal aberrations in which allura red significantly induced the highest frequencies of abnormal prophase, sticky metaphase, c-mitosis, sticky anaphase, and laggard anaphase at each concentration. While, dislocated metaphase, forwarded anaphase, and bridge anaphase were only found in sunset yellow, tartrazine, and fast green, respectively. The use of evaluated FCAs especially allura red in foodstuff, cosmetics, and skincare products can cause cancer in humans.

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Genotoxicity and cytotoxicity analysis of curcumin and sunset yellow in human lymphocyte culture

Cited by 10 publications

References 27 publications

Effects of Curcumin and Luteolin on Viability and Cell Death Induction in NFS-60 Cell Line

Effects of Curcumin and Luteolin on Viability and Cell Death Induction in NFS-60 Cell Line

Food Additives and Genotoxicity

Geno-Toxic Appraisal of Widely Used Food Color Additives on Model Plant Allium cepa Root Tip Cells

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