Genotoxicity and antigenotoxicity of cashew (Anacardium occidentale L.) in V79 cells

Evaluation of mutagenicity and antimutagenicity of cashew stem bark methanolic extract in vitro

“…The reduction in the mean CA frequencies obtained in the present study in treatments realized throughout the entire cycle and in the cell cycle phases G1 and S, corroborate the data obtained in the comet assay previously realized by our group on V79 cells exposed to CSBME and MMS (Barcelos et al, 2007). Therefore, the mechanism through which the compounds present in the CSBME protect DNA is possibly of bioantimutagenesis type, i.e., the compounds present in the extract probably increase replication fidelity of DNA (Kada et al, 1982).…”

“…In a work previously realized by our group (Barcelos et al, 2007), MMS was also used as a damage-inducing agent in V79 cells and showed that CSBME reduced the primary damage caused by MMS. It was suggested that CSBME acted as a bioantimutagenic agent, since it was more efficient in cells treated simultaneously with the extract and MMS and in cells treated with CSBME after 2 h of treatment with MMS.…”

“…The concentrations of 500, 1000 and 2000 g/mL of culture environment were used for evaluation of the mutagenic activities and antimutagenic from this extract and were determined previously from the experiments of citotoxity (Barcelos et al, 2007).…”

Pharmacological properties of cashew (Anacardium occidentale)

“…Cashew pulp juice and methanol extract of stem bark (500-2000 µg/ml) have antigenotoxic activity against Salmonella typhimurium and Chinese hamster lung fibroblasts (V79 cells), respectively (Barcelos et al, Leite et al 18572007a. According to Melo-Cavalcante et al (2005), cashew pulp (cajuína) protected S. typhimurium (TA102) from damage induced by aflatoxin B1 (AFB1), while the methanolic extract of bark (500-2000 µg/ml) of Chinese hamster (V79) in doxorubicin (0.75 mg/ml) induced damage (Barcelos et al, 2007b).…”

Anacardium occidentale

“…Studies using the Comet assay on Chinese hamster lung fibroblasts (V79 cells) revealed that the two lowest concentrations of cashew stem bark methanolic extract (500 mg/ml, 1,000 mg/ ml) tested presented no genotoxic activity, whereas the highest dose of 2,000 m/ml produced genotoxicity (Barcelos et al 2007a). All of the concentrations showed protective activity in simultaneous and post-treatment in relation to methyl methanesulfonate (MMS) as a positive control.…”