Genotoxic potential and in vitro tumour-promoting potential of 2-dodecylcyclobutanone and 2-tetradecylcyclobutanone, two radiolytic products of fatty acids

Yamakage, Kohji; Sui, Hajime; Ohta, Ryo; Toyoizumi, Tomoyasu; Kawakami, Kumiko; Matsumoto, Hirotaka; Takahashi, Toshitaka; Sasaki, Kiyoshi; Ikezumi, Mayu; Negishi, Saki; Izumi, Kiyotaka; Todoriki, Setsuko; Kondo, Takashi; Furuta, Mamoru

doi:10.1016/j.mrgentox.2014.04.021

Cited by 7 publications

(5 citation statements)

References 33 publications

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“…They also stated that possible chronic toxicity remains to be evaluated [ 48 ]. Most often these are mutagenesis tests on bacterial strains in Petri dishes (Ames tests) which are only interested in the genotoxic effect, other authors indicating that it is the carcinogenesis-promoting effect of 2-alkylcyclobutanones that poses a real problem [ 100 ]. Finally, this subject is controversial, and the debate must remain open [ 101 ].…”

Section: Controversies Concerning Consumer Perception and Acceptanmentioning

confidence: 99%

Control of Foodborne Biological Hazards by Ionizing Radiations

Munir

Fédérighi

2020

Foods

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Ionization radiations are used to ensure food safety and quality. This irradiation process uses ions of beta or gamma rays to inactivate or destroy the food spoilage pests, microorganisms and their toxins without significantly increasing the temperature of the treated product. Meanwhile, various intrinsic and extrinsic factors are involved in determining the efficacy of ionization irradiation against these organisms. Therefore, the dose of radiations is recommended according to the type of irradiation, substrate and microorganisms. However, controversies are surrounding the use of irradiations in the food industry due to a negative perception of irradiations. This manuscript described the use of ionization radiations to control the foodborne biological hazards and increase shelf life. Firstly, the characteristics and mode of action of irradiations were discussed. Secondly, the role of extrinsic and intrinsic factors influencing the radioresistance of biological hazards were elaborated. This literature review also detailed the differential effects of irradiations on different microorganisms and pests having a role in food safety and deterioration. Finally, the regulatory status and the consumer values along with the controversies surrounding the use of ionization irradiations in the food sector were explained.

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Section: Controversies Concerning Consumer Perception and Acceptanmentioning

confidence: 99%

Control of Foodborne Biological Hazards by Ionizing Radiations

Munir

Fédérighi

2020

Foods

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“…On the other hand, Sommers () tested in 2 first lines E. coli and then in a later article Sommers and Schiestl () has completed its experiment by using S. typhimurium cell lines. We can mention Yamakage and others () as a reference of how to perform correctly all the suggestions for a complete and reliable study, the group used all necessary lines, most suitable board type, and a wide range of dosages, and as other studies also showed negative for mutagenicity of 2‐DCB compounds and 2‐tDCB.…”

Section: Discussionmentioning

confidence: 99%

Ames Test to Detect Mutagenicity of 2‐Alkylcyclobutanones: A Review

Barbezan

Martins

Bueno

et al. 2017

Journal of Food Science

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Food irradiation is an effective and safe method for preservation and long-term storage, and it is approved for use in over 60 countries for various applications in a wide variety of food products. This process is performed by use of accelerated electron beams, X-rays, or gamma radiation ( 60 Co or 137 Cs). 2-Alkylcyclobutanones (2-ACBs) are the only known radiolytic products generated from foods that have fatty acids (triglycerides) and are subjected to irradiation. Since the 1990s toxicological safety studies of 2-ACBs have been conducted extensively through synthetic compounds, then and tests to determine if the compounds have any mutagenic activity are strictly necessary. The Ames test was chosen by many researchers to assess the mutagenicity of 2-ACBs. The test uses distinct bacterial cell lines Salmonella typhimurium to detect point mutations at sites guanine-cytosine (G-C) and Escherichia coli to detect point mutations at sites adenine-thymine (A-T). This bibliographic research aims to bring together all the results obtained and a comparison and cell lines used, type of plates, and solvents. This research showed that no mutagenic activity was observed in any of the cell lines and concentrations evaluated by the works of authors, so the 2-ACBs compounds showed no mutagenic substance in concentrations detectable by the Ames test.

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“…As concentrações de 2-ACBs nos alimentos são da ordem de partes por milhão, por exemplo, a quantidade de 2-dDCB (2-Dodecilciclobutanona), que é derivada do ácido palmítico, foi estimada em 12,9 µg por 100 g de carne de frango irradiada a 3 kGy (Yamakage et al, 2014).…”

Section: Irradiação De Alimentosunclassified

“…Os A única equipe que utilizou a técnica do ensaio do micronúcleo para determinar possível genotoxicidade causada pelas 2-ACBs disponível na literatura foi liderado por Yamakage et al (2014), que fez uso de células derivadas de medula óssea de rato após os mesmos receberem os compostos via oral. O resultado obtido foi negativo para células de medula óssea, importante em se tratando de avaliar efeito sistêmico.…”

Section: Avaliação Da 2-ddcb -24hunclassified

“…Ao mesmo tempo, os poucos estudos existentes disponíveis sobre possíveis efeitos promotores carcinogênicos das 2-ACBs não são suficientes e são de suma importância que pesquisas sobre suas propriedades toxicológicas e metabólicas fossem mais aprofundadas, por menor que seja o risco, não só em células de cólon como demonstraram estudos já realizados, bem como em todos os órgãos envolvidos no processo digestório e que acumulam gordura, para acalmar definitivamente preocupações públicas relacionadas ao consumo de alimentos irradiados.Poucos estudos foram realizados referentes à avaliação dos possíveis efeitos genotóxicos das 2-ACBs. Ao que consta na literatura a maior parte dessas avaliações foram feitas por meio do teste do cometa e somente um grupo liderado porYamakage et al (2014) utilizou o ensaio do MN para esta finalidade. Diferentemente deste estudo em questão, a técnica utilizada por eles não foi a mesma, bem como o material avaliado.…”

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Estudos <i>in vitro </i> da genotoxicidade e citotoxicidade em células hepáticas da formação de 2-alcilciclobutanonas resultantes da irradiação de alimentos que contenham gordura

Barbezan¹

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Food irradiation has already been approved and has been used in several countries for applications and purposes of a wide variety of foods. Its benefits include increased shelf life, improved food hygiene and consequently less deterioration and losses compared to foods that do not undergo into radiation. In addition, food after irradiation is safe in terms of nutrients and pathogen reduction. However, foods that contain medium to high fat levels, induce the formation of a by-product called 2-Alkylcyclobutanones, which we know that part of these ingested compounds are normally excreted through the feces, but part remains deposited in the adipose tissues. Work performed with these compounds previously showed cytotoxic and genotoxic effects on colon cells. Thus, the objective of the present work was to investigate the cytotoxic effects performed in cell viability tests, genotoxic tests in micronucleus and mutagenic tests with Ames technique under in vitro experimental conditions of 2-dDCB and 2-tDCB compounds. Hence, the liver was the chosen organ to evaluate the possible effects of these compounds, since this organ is usually affected by accumulation of fat. Three hepatic cell lines were used: HepG2, BRL3A and HTC. Analysis of the cell viability results revealed that the 2-dDCBs presented a discrete cytotoxic effect at the concentration of 500 μM and the 2-tDCBs presented low damages from 100 μM and larger at 500 μM, showing to be dose dependent. In the mutagenicity results, the compounds did not show any mutagenic effects at the concentrations and doses used, detected by the Ames test. Finally, the micronucleus test corresponded to expectations demonstrating no genotoxic effects in the cell line, doses and times tested. Based on the results achieved, the 2 ACBs can be consumed with relative safety, from the perspective of possible mutagenic and genotoxic effects in the evaluated concentrations.

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Genotoxic potential and in vitro tumour-promoting potential of 2-dodecylcyclobutanone and 2-tetradecylcyclobutanone, two radiolytic products of fatty acids

Cited by 7 publications

References 33 publications

Control of Foodborne Biological Hazards by Ionizing Radiations

Control of Foodborne Biological Hazards by Ionizing Radiations

Ames Test to Detect Mutagenicity of 2‐Alkylcyclobutanones: A Review

Estudos <i>in vitro </i> da genotoxicidade e citotoxicidade em células hepáticas da formação de 2-alcilciclobutanonas resultantes da irradiação de alimentos que contenham gordura

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