Genotoxic evaluation of pirfenidone using erythrocyte rodent micronucleus assay

Alcántar-Díaz, Blanca Estela; Gómez‐Meda, Belinda Claudia; Zúñiga‐González, Guillermo Moisés; Zamora‐Pérez, Ana Lourdes; Gonzalez-Cuevas, J.; Alvarez-Rodríguez, Bertha A; Sánchez-Parada, María Guadalupe; García-Bañuelos, Jesús; Armendáriz‐Borunda, Juan

doi:10.1016/j.fct.2012.05.049

Cited by 7 publications

(7 citation statements)

References 27 publications

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“…Moringa oleifera has antioxidant activity because it contains phenolic compounds and flavonoids, specifically three classes of phytochemicals: glucomoringin, flavonoids (quercetin and kaempferol) and phenolic acids (chlorogenic acid) [41]. These bioactive compounds can exert antioxidant and anti-inflammatory effects that could induce cellular protection [42, 43], as can be observed in M. oleifera regulation of the formation of micronuclei in response to damage to the genetic material in cells. Micronuclei are chromosomal fragments or entire chromosomes that were not included into the daughter cell nuclei at mitosis.…”

Section: Discussionmentioning

confidence: 99%

Effect of Moringa oleifera consumption on diabetic rats

Villarruel-López

Mora

Vázquez-Paulino

et al. 2018

BMC Complement Altern Med

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BackgroundTherapeutic use of leaves of M. oleifera has been evaluated in diabetes because of its possible capacity to decrease blood glucose and lipids concentration after ingestion, as result of the polyphenols content and others compounds. Nevertheless most results have been obtain from leaf extract, therefore this study would use leaf powder as the regular way of consumption of population to know effects over toxicity glucose, triglycerides, cholesterol, corporal weight, and predominant groups of microbiota.MethodsPowdered leaf was administrated in different doses to know toxicity and genotoxicity using LD50 and micronuclei assay. Hyperglycemia was induced by alloxan on Sprague Dawley rats. Glucose and body weight were measured once a week meanwhile cholesterol and triglycerides were analyzed at the end of the study by commercial kits. Different organs were examined by hematoxylin-eosin technique. Lactic acid bacteria and Enterobacteriaceae were enumerated from stool samples.ResultsThe tested doses revealed no lethal dose and no significant differences in genotoxicity parameter. The consumption of the leaves showed a hypoglycemic effect (< 250 mg/dL in diabetic M. oleifera treated group), however in corporal weight showed an increased (> 30 g over no M. oleifera treated groups). There was no change in enumeration of lactic acid bacteria (8.4 CFU/g) but there were differences in the predominance of type of lactobacillus and enterobacteria enumeration.ConclusionsThese results help to increase information over the most popular use of M. oleifera and its safety. However there are needed more studies over the hypoglycemic mechanisms and effects over intestinal microbiota.

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Section: Discussionmentioning

confidence: 99%

Effect of Moringa oleifera consumption on diabetic rats

Villarruel-López

Mora

Vázquez-Paulino

et al. 2018

BMC Complement Altern Med

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“…In this case, newborn animals were more susceptible, and the effects of the damage were very clear [17, 19, 20, 33, 40]. …”

Section: Discussionmentioning

confidence: 99%

“…Female adult rats were mated, and pregnancy was established by the presence of sperm in a vaginal smear [19, 20]. The pups' day of birth was calculated once pregnancy was confirmed.…”

Section: Methodsmentioning

confidence: 99%

Micronucleated Erythrocytes in Peripheral Blood from Neonate Rats Exposed by Breastfeeding to Cyclophosphamide, Colchicine, or Cytosine-Arabinoside

Gómez‐Meda

Bañales-Martínez

Zamora‐Pérez

et al. 2016

BioMed Research International

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Genotoxic exposure to chemical substances is common, and nursing mothers could transmit harmful substances or their metabolites to their offspring through breast milk. We explored the possibility of determining genotoxic effects in the erythrocytes of breastfeeding rat pups whose mothers received a genotoxic compound while nursing. Ten groups of female rats and five pups per dam were studied. The control group received sterile water, and the experimental groups received one of three different doses of cyclophosphamide, colchicine, or cytosine-arabinoside. Blood smears were prepared from samples taken from each dam and pup every 24 h for six days. There were increased numbers of micronucleated erythrocytes (MNEs) and micronucleated polychromatic erythrocytes (MNPCEs) in the samples from pups in the experimental groups (P < 0.02) and increased MNPCE frequencies in the samples from the dams (P < 0.05). These results demonstrate the vertical transmission of the genotoxic effect of the compounds tested. In conclusion, assessing MNEs in breastfeeding neonate rats to assess DNA damage may be a useful approach for identifying genotoxic compounds and/or cytotoxic effects. This strategy could help in screening for therapeutic approaches that are genotoxic during the lactation stage and these assessments might also be helpful for developing preventive strategies to counteract harmful effects.

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“…Each female rat was flushed daily with a vaginal wash of 0.1 ml of sterile water using an adjustable-volume pipettor, and the contents were smeared onto clean slides, which were analyzed without stain using a light microscope. The day of the initial identification of sperm was established as the first day of the pregnancy after which female rats were housed in individual cages, and a dose administration schedule was assigned for each rat [18, 19] (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

Genome Damage in Rats after Transplacental Exposure to Jatropha dioica Root Extract

Morales-Velazquez

Lazalde-Ramos

Gómez‐Meda

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Jatropha dioica is traditionally used owing to its antiviral, antifungal, and antimicrobial properties. But, toxicological information regarding J. dioica root total extract is currently limited. The aim of this work was to evaluate in a rat model, the transplacental genotoxicity effect of J. dioica aqueous root total extract. Three different J. dioica aqueous root total extract doses (60, 100, and 300 mg/kg) were administered orally to Wistar rats during 5 days through the pregnancy term (16–21 days). Pregnant rats were sampled every 24 h during the last 6 days of gestation, and pubs were sampled at birth. Genome damage in dams and their newborn pups transplacentally exposed to J. dioica was evaluated by in vivo micronuclei assay. We evaluated the frequency of micronucleated erythrocytes (MNE), micronucleated polychromatic erythrocytes (MNPCE), and polychromatic erythrocytes (PCE) in peripheral blood samples from pups and MNPCE and PCE in pregnant rats. No genotoxic effect was observed after oral administration of the three different doses of aqueous root total extract of J. dioica in pregnant or in their newborn pubs, after transplacental exposure. A significant decrease in PCE frequency was noted in samples from pubs of rats treated with the highest dose of J. dioica extract. The aqueous total root extract of J. dioica at the highest dose tested in our research do have cytotoxic effect in pups transplacentally exposed to this plant extract. Moreover, neither a genotoxic nor a cytotoxic effect was observed in pregnant rats. In the present work, there was no evidence of genome damage in the rat model after transplacental exposure to J. dioica aqueous root total extract.

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Genotoxic evaluation of pirfenidone using erythrocyte rodent micronucleus assay

Cited by 7 publications

References 27 publications

Effect of Moringa oleifera consumption on diabetic rats

Effect of Moringa oleifera consumption on diabetic rats

Micronucleated Erythrocytes in Peripheral Blood from Neonate Rats Exposed by Breastfeeding to Cyclophosphamide, Colchicine, or Cytosine-Arabinoside

Genome Damage in Rats after Transplacental Exposure to Jatropha dioica Root Extract

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