Genotoxic Effects of Semi-Synthetic Isodillapiole on Oviposition in Aedes aegypti (Linnaeus, 1762) (Diptera: Culicidae)

Abstract

INTRODUCTION: Semi-synthetic dillapiole compounds derived from Piper aduncum essential oil are used as alternative insecticides to control insecticide-resistant Aedes aegypti . Thus, we aimed to evaluate the genotoxic effects of semi-synthetic isodillapiole on the nuclei of neuroblasts (larvae) and oocytes (females) and the mean oviposition rates of the females over four generations (G 1 , G 2 , G 3 , and G 4 ) of Ae. aegypti . METHODS: Larvae were captured in the city of Manaus, Amazonas state, Brazil, and exposed to isodillapiole in bioassays (20, 40, and 60 µg/mL) and a negative control (0.05% DMSO in tap water) for 4 h. The cerebral ganglia were extracted from the larvae and oocytes from the adult females to prepare slides for cytogenetic analysis. Breeding pairs were established and eggs counts were quantified taken after the bioassays. RESULTS: The analysis of 20,000 interphase nuclei of neuroblasts and oocytes indicated significant genotoxicity (micronuclei, budding, polynucleated cells, and other malformations) compared to that of the control. Metaphasic and anaphasic nuclei presented chromosomal breaks; however, no significant variation and damage was observed in the negative control. A significant reduction in mean oviposition rates was also recorded following exposure to isodillapiole over the four generations (G 1 , G 2 , G 3 , and G 4 ). CONCLUSIONS: The toxic and genotoxic effects of isodillapiole on Ae. aegypti were caused by reduced oviposition in the females and nuclear abnormalities over the four generations of the trials. Further studies are required, rather than our in vitro assays, to verify the efficacy of exposure to this compound for controlling Ae. aegypti.

Genotoxic and mutagenic effects of methyl ether dillapiole on the development of Aedes aegypti (Diptera: Culicidae)

“…Likewise, dos Santos et al . (2020) observed for four generations that the isodillapiole derivative caused a toxic and genotoxic effect in Ae. aegypti , evidenced by nuclear and chromosomes abnormalities and a reduction in oviposition.…”

“…dos Santos et al . (2020) also observed that isodillapiole, another derivative of dillapiole, had a toxic and genotoxic effect on Ae. aegypti .…”

Educação em saúde para o controle de Aedes aegypti

“…Já os semissintéticos: E-isodilapiol (ED), éter metil dilapiol (EMD), éter etil dilapiol (EED) e éter n-butil dilapiol (EBD) têm atividade ovicida, larvicida e mutagênica em ovos e larvas de Ae. aegypti (DOMINGOS et al, 2014;SANTOS et al, 2020) e em Ae. albopictus (MEIRELES et al, 2016).…”

“…Estudos toxicológicos e de genotoxicidade de biomarcadores, voltados ao controle de mosquitos, têm apresentado resultados promissores (DOMINGOS et al, 2014, SANTOS et al, 2020. Meireles et al (2016) observaram alta mortalidade de larvas de Ae.…”

A importância da equipe multidisciplinar para administração de nutrientes por via parenteral em Unidade de Terapia Intensiva – UTI

“…No entanto, os mosquitos têm apresentado resistência, o que pode estar relacionado a uma redução na taxa de penetração do inseticida ou em seu metabolismo . Nesse sentido, pesquisas alternativas, utilizando compostos naturais à base de plantas, óleos essenciais e/ou seus derivados, têm emergido, para o controle de mosquitos vetores de doenças (MEIRELES et al, 2016;SANTOS et al, 2020).…”