Genotoxic effects of chromium(VI) and cadmium(II) in human blood lymphocytes using the electron microscopy in situ end-labeling (EM-ISEL) assay

Depault, François; Cojocaru, Marilena; Fortin, Fléchère; Chakrabarti, Swapan; Lemieux, Nicole

doi:10.1016/j.tiv.2005.09.003

Cited by 41 publications

(16 citation statements)

References 30 publications

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“…DNA damage observed in this study coincides with the results of previous studies on human lymphocytes that demonstrated an increase in DNA migration pattern and single strand breaks at different non cytotoxic concentrations of Cd (Mouron et al, 2001;Depault et al, 2006). Moreover, Celik et al (2009) found that Cd chloride administration induced genetic damage in both BM and peripheral blood with increase in DNA single strand breaks indicating an interaction between DNA and Cd chloride.…”

Section: Discussionsupporting

confidence: 90%

The Different Responses of Some Adult Albino Rats’ Body Organs to the Genotoxic Effect of Cadmium

Elhaleem

Ahmed

2012

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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Cadmium (Cd) is one of the most toxic environmental pollutants affecting the DNA. Cadmium interferes with DNA repair process and induces oxidative stress leading to DNA damage. Since the most recent molecular cancer genetic and cytogenetic data have provided strong evidence that genetic damage can be the initial step of the malignant process, the early detection of DNA-damaging effect of Cd in different organs is very important to avoid its carcinogenic potential .Hence, this study was undertaken to determine the response of some different rat organs to the genotoxic effect of Cd. The study was conducted on 48 adult male albino rats divided into three main groups; group I (negative control group) consisted of 15 rats, group II (positive control group) consisted of 15 rats, received 1 ml distilled water daily by gavage and group III (cadmium group) consisted of 18 rats, received cadmium chloride in a dose of 5 mg/kg body weight daily by gavage. Each one of these three main groups was subdivided according to the experimental period into three equal subgroups; (a), (b), and (c). The experimental period was two weeks for subgroups Ia, IIa, and IIIa, four weeks for subgroups Ib, IIb, and IIIb and eight weeks for subgroups Ic, IIc, and IIIc. Determination of possible DNA damage using comet assay in lymphocytes, bone marrow (BM), lung, liver, and kidney along with malondialdehyde and Cd levels were carried out. The results of this study revealed a heterogeneous response of the studied organs to the genotoxic effect of Cd. Lymphocytes and BM were early affected while the liver showed the highest genetic damage with longer exposure period. These findings suggest that lymphocytes and BM are more sensitive to Cd genotoxicity than the lung, liver, and kidney. So lymphocytes and BM can be used as early determinants of Cd-induced genetic damage. Considering these issues, health educational programs should be implemented to highlight the dangerous impact of Cd on health. Moreover, persistence of this metal in the environment requires a long-term move towards minimizing human exposure through environmental management and preservation of lower Cd levels wherever possible. Also, further studies are needed to detect the genotoxicity of Cd in other organs.

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Section: Discussionsupporting

confidence: 90%

The Different Responses of Some Adult Albino Rats’ Body Organs to the Genotoxic Effect of Cadmium

Elhaleem

Ahmed

2012

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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“…3 Cadmium (Cd 2+ ) is another highly toxic metal, which can lead to pathological problems with central nervous system, kidney's filtering system, and immune system. 4,5 For these reasons, it is vitally important to develop techniques for reliable monitoring of the heavy metals in the environment, drinking water, food, and biological liquids. Despite high sensitivity and acceptable selectivity, conventional bulky methods for heavy metal detection have several drawbacks, including time-consuming sample preparation procedure, strong demand in training professional staff, high cost of the detection tools.…”

Section: Introductionmentioning

confidence: 99%

Insights into the origin of the excited transitions in graphene quantum dots interacting with heavy metals in different media

Shtepliuk

Khranovskyy

Yakimova

2017

Phys. Chem. Chem. Phys.

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Exploring graphene quantum dots (GQDs) is an attractive way to design novel optical and electrochemical sensors for fast and reliable detection of toxic heavy metals (HMs), such as Cd, Hg and Pb. There are two main strategies for achieving this: (i) surface modification of an electrochemical working electrode by nanoscale GQDs and (ii) using a GQD solution electrolyte for optical sensing. Further development of these sensing technologies towards reaching or exceeding the WHO permissible limits implies deep understanding of the interaction between GQDs and HMs in different dielectric media. Solvent is expected to be one of the key factors affecting the binding ability of the GQDs to HMs and their electronic and optical properties. Here we show that the solvent-solute interaction changes the geometrical configuration, stability and absorption spectra of zigzag/armchair-edged GQDs after complexation with neutral and charged HM species. We observe physisorption behavior of Cd and Hg adatoms on the sp surface with a solvent-mediated enhancement of the binding energy with increasing solvent polarity. For Pb adatoms, an opposite picture is revealed. We find that the solvent effect also manifests itself in weakening of the chemisorption strength in the HM cation-π system with increasing dielectric constant of the solvent. Thus, a solvent engineering strategy based on control of the dielectric permittivity can be a promising approach to reach the desired binding energy in the HM@GQDs and to provide high sensitivity and selectivity of both optical and electrochemical sensors to toxic HMs we are interested in.

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“…Chromium is a recognized carcinogen of the respiratory tract, lungs, and gastric mucosa epithelium [50][51][52][53][54][55][56]. Higher levels of Cr were found in blood plasma of people suffering from lymphoproliferative diseases [57], as well as hexavalent Cr genotoxicity measured by the amount of chromosome aberrations [58,59] and the amount of single and/or double-strand DNA breakage in human lymphocytes in vitro [60,61] and animal cells [62,63]. Maeng et al [64] indicated growth of exchange frequency of sister chromatids in genetic material, and Vaglenov et al [65] proved substantial increase of micronucleus frequency occurrence in the micronucleus test on lymphocytes (MN) in workers exposed to chromium.…”

Section: Correlation Coefficients Between Elements In Rainwater Andmentioning

confidence: 99%

The Content of Elements in Rainwater and Its Relation to the Frequency of Hospitalization for Chronic Lymphocytic Leukemia and Chronic Myeloid Leukemia in Opole Voivodship, Poland, During 2000–2002

Szyguła

Bunio

Tubek

2010

Biol Trace Elem Res

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Environmental factors play an essential role in the etiology of diseases of the hematopoietic system. Such factors include soil and water pollution and the presence of metals and toxic compounds in the air. Measuring the content of metallic elements in rainwater has become an accepted procedure for environmental pollution monitoring. In accordance with the above, it was decided to study relations between the content of selected elements in rainwater and hospitalization frequency due to chronic lymphocytic leukemia (CLL, C91 on ICD-10) and chronic myeloid leukemia (CML, C92 on ICD-10). It can be assumed that hospitalization frequency is a reliable indicator of exacerbations of these diseases. The annual average of hospitalizations due to a given disease during the years 2000-2002 was correlated with the annual average content of a given element in rainwater using the Spearman's correlation indicator to describe the relationship between the element content and the disease that is possibly a consequence of the element's presence in rainwater. In cases of CLL for all the subjected population and for men, no statistically significant correlations were found. For women, statistically significant correlations were found for chromium (r = 0.66), lead (r = 0.58), copper (r = 0.58), and cadmium (r = 0.51). For CML in all the studied population significant, negative correlations were found for magnesium (r = -0.6) and zinc (r = -0.52). In men, significant negative correlations were seen for magnesium (r = -0.69 and zinc (r = -0.55). No significant correlations were found in women. These results indicate the need of taking into account the environmental and gender factors in research connected with these diseases, which can be probably of help in improvements of therapy efficiency.

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Genotoxic effects of chromium(VI) and cadmium(II) in human blood lymphocytes using the electron microscopy in situ end-labeling (EM-ISEL) assay

Cited by 41 publications

References 30 publications

The Different Responses of Some Adult Albino Rats’ Body Organs to the Genotoxic Effect of Cadmium

The Different Responses of Some Adult Albino Rats’ Body Organs to the Genotoxic Effect of Cadmium

Insights into the origin of the excited transitions in graphene quantum dots interacting with heavy metals in different media

The Content of Elements in Rainwater and Its Relation to the Frequency of Hospitalization for Chronic Lymphocytic Leukemia and Chronic Myeloid Leukemia in Opole Voivodship, Poland, During 2000–2002

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