Genotoxic effects of aluminum chloride in cultured human lymphocytes treated in different phases of cell cycle

Lima, Patrícia Danielle Lima de; Leite, D.S.; Vasconcellos, Marne Carvalho de; Cavalcanti, Bruno C.; Santos, Raquel; Costa-Lotufo, Letı́cia V.; Pessoa, Cláudia; Moraes, Manoel Odoríco de; Burbano, Rommel Rodrı́guez

doi:10.1016/j.fct.2006.12.022

Cited by 85 publications

(62 citation statements)

References 34 publications

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“…Al treatment caused an increased DNA damage as indicated by the increased fragmentation of DNA and the number of comets observed. DNA fragmentation and increase in the appearance of comets have also been reported in other studies as a consequence of Al exposure (Lima et al 2007). Al is known to increase the levels of reactive oxygen species (Bondy and Kirstein 1996;Bondy et al 1998) as well as nitrogen species as seen in the present study also, which are known to cause damage to various macromolecules and also to DNA.…”

Section: Discussionsupporting

confidence: 78%

Potential of lithium to reduce aluminium-induced cytotoxic effects in rat brain

2009

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The present study was aimed to explore the potential of an antidepressant drug lithium (Li) in reducing aluminium (Al) induced neurotoxicity. To carry out the investigations, Al was administered orally (100 mg AlCl(3)/Kg b wt/day) whereas, Li was administered through diet (1.1 g Li(2)CO(3)/Kg diet, daily) for a total duration of 2 months. Al treatment resulted in a significant increase in the activity of enzyme nitric oxide synthase and the levels of L-citrulline which, however, were decreased appreciably following lithium supplementation. Al treatment also revealed an increase in DNA fragmentation as evidenced by an increase in number of comets. Interestingly, Li supplementation to Al treated rats reduced the damage inflicted on DNA by Al. Ultrastructural studies revealed an increase in chromatin condensation with discontinuity in nuclear membrane in both the cerebrum and cerebellum of Al treated rats which showed improvement following Li supplementation. Alterations in the structure of synapse and mitochondrial swelling were also seen. The present study shows the potential of Li in containing the damage inflicted by Al on rat brain.

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Section: Discussionsupporting

confidence: 78%

Potential of lithium to reduce aluminium-induced cytotoxic effects in rat brain

2009

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“…The study indicated that AlCl3 induces DNA damage and is cytotoxic during all phases of the cell cycle. Also, the treatment of the cells at G1 phase resulted in polyploidy and endoreduplication, consistent with AlCl3 interacting with the mitotic spindle apparatus (Lima et al, 2007). These data, along with the results of other studies reported in the literature, indicates that AlCl3 is genotoxic and should be used with caution.…”

Section: Genotoxicity Of Aluminumsupporting

confidence: 86%

“…These results suggests, in a contrary way to the study of Scalon et al (2011), that Al caused deleterious DNA and cellular effects in aquatic organisms. Recently our research group published a study on the genotoxic, clastogenic and cytotoxic effects of AlCl3 in different phases of the cell cycle using in vitro temporary cultures of human lymphocytes (Lima et al, 2007). Moreover, the mitotic index (MI), chromosomal aberrations (CAs) and DNA damage index were analyzed by the comet assay.…”

Section: Genotoxicity Of Aluminummentioning

confidence: 99%

Alzheimer’s Disease and Metal Contamination: Aspects on Genotoxicity

Lima¹,

Vasconcellos²,

Montenegro³

et al. 2011

Alzheimer's Disease Pathogenesis-Core Concepts, Shifting Paradigms and Therapeutic Targets

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“…In addition, specifi c metal compounds exhibit unique mechanisms such as interruption of cell-cell adhesion by cadmium, direct DNA binding of trivalent chromium produced from hexavalent chromium [also see (27)], or perturbation of the mitotic spindle or inhibition of its formation. The latter holds true for Cr +6 products (Na 2 CrO 4 and CaCrO 4 ) and aluminium chloride (28,29).…”

Section: Figure 2 Micronucleus Frequency In Human Peripheral Blood Lymentioning

confidence: 89%

In Vitro Genotoxicity of Wastewaters from the Town of Settat, Morocco

Asslouj

Amahdar

Glouib

et al. 2009

Archives of Industrial Hygiene and Toxicology

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In recent years, the town of Settat has seen a considerable industrial growth, which has resulted in increased environmental pollution. This includes pollution by household and industrial wastewaters, which are released into the Boumoussa River without any preliminary treatment. The river valley crosses the community of Mzamza 8 km to the north of the town. Years of drought forced members of the community to use this polluted ground water for irrigation and put themselves and the environment at risk. The aim of this study was to determine the physicochemical and metal profi le of Settat wastewaters and to assess their impact on the water table. The second objective was to investigate the genotoxic potential of wastewater on human peripheral blood lymphocytes in vitro, using the micronucleus test and cellular proliferation index. This study demonstrated signifi cant pollution of Boumoussa valley groundwater and of the local wells. Sampled water induced a clear increase in the frequency of micronucleated cells and a lower cell proliferation in human peripheral blood lymphocytes in vitro.

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Genotoxic effects of aluminum chloride in cultured human lymphocytes treated in different phases of cell cycle

Cited by 85 publications

References 34 publications

Potential of lithium to reduce aluminium-induced cytotoxic effects in rat brain

Potential of lithium to reduce aluminium-induced cytotoxic effects in rat brain

Alzheimer’s Disease and Metal Contamination: Aspects on Genotoxicity

In Vitro Genotoxicity of Wastewaters from the Town of Settat, Morocco

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