Genotoxic effects and molecular docking of 1,4-dioxane: combined protective effects of trans-resveratrol

Kurt, Deniz; Acar, Ali; Çavuşoğlu, Dilek; Yalçın, Emine; Çavuşoğlu, Kültiğin

doi:10.1007/s11356-021-14387-3

Cited by 18 publications

(6 citation statements)

References 54 publications

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“…Root MDA levels were measured by applying the method suggested by Unyayar et al 19 and the MDA levels are shown as µM/g FW. GSH levels were analyzed by sulfhydryl level measurement as described by Kurt et al 20 and expressed as µmol/g. For enzyme activity measurements, enzyme extraction procedure was carried out at + 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Dose-dependent toxicity profile and genotoxicity mechanism of lithium carbonate

Kuloğlu

Yalçın

Çavuşoğlu

et al. 2022

Sci Rep

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The increasing widespread use of lithium, which is preferred as an energy source in batteries produced for electric vehicles and in many electronic vehicles such as computers and mobile phones, has made it an important environmental pollutant. In this study, the toxicity profile of lithium carbonate (Li2CO3) was investigated with the Allium test, which is a bio-indicator test. Dose-related toxic effects were investigated using Li2CO3 at doses of 25 mg/L, 50 mg/L, and 100 mg/L. The toxicity profile was determined by examining physiological, cytotoxic, genotoxic, biochemical and anatomical effects. Physiological effects of Li2CO3 were determined by root length, injury rate, germination percentage and weight gain while cytotoxic effects were determined by mitotic index (MI) ratio and genotoxic effects were determined by micronucleus (MN) and chromosomal aberrations (CAs). The effect of Li2CO3 on antioxidant and oxidant dynamics was determined by examining glutathione (GSH), malondialdehyde (MDA), catalase (CAT) and superoxide dismutase (SOD) levels, and anatomical changes were investigated in the sections of root meristematic tissues. As a result, Li2CO3 exhibited a dose-dependent regression in germination-related parameters. This regression is directly related to the MI and 100 mg/L Li2CO3 reduced MI by 38% compared to the control group. MN and CAs were observed at high rates in the groups treated with Li2CO3. Fragments were found with the highest rate among CAs. Other damages were bridge, unequal distribution of chromatin, sticky chromosome, vagrant chromosome, irregular mitosis, reverse polarization and multipolar anaphase. The genotoxic effects were associated with Li2CO3-DNA interactions determined by molecular docking. The toxic effects of Li2CO3 are directly related to the deterioration of the antioxidant/oxidant balance in the cells. While MDA, an indicator of lipid peroxidation, increased by 59.1% in the group administered 100 mg/L Li2CO3, GSH, which has an important role in cell defense, decreased by 60.8%. Significant changes were also detected in the activities of SOD and CAT, two important enzymes in antioxidant defense, compared to the control. These toxic effects, which developed in the cells belonging to the lithium-treated groups, were also reflected in the tissue anatomy, and anatomical changes such as epidermis cell damage, cortex cell damage, flattened cell nucleus, thickening of the cortex cell wall and unclear vascular tissue were observed in the anatomical sections. The frequency of these changes also increased depending on the Li2CO3 dose. As a result, Li2CO3, which is one of the lithium compounds, and has become an important contaminant in the environment with increasing technological developments, caused a combined and versatile toxicity in Allium cepa L. meristematic cells, especially by causing deterioration in antioxidant/oxidant dynamics.

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Section: Methodsmentioning

confidence: 99%

Dose-dependent toxicity profile and genotoxicity mechanism of lithium carbonate

Kuloğlu

Yalçın

Çavuşoğlu

et al. 2022

Sci Rep

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“…We observed green asparaguses were associated with accumulation of alpha-phellandrene, which was shown characteristic dill flavor [ 25 ]. Kurt et al [ 26 ] showed that the hydrogen bonds and hydrophobicity between 1,4-dioxane and antioxidant enzymes (such as superoxide dismutase and catalase) resulted in a strong interaction and binding affinity; therefore, Grace may have higher clearance rates for DPPH· and ·OH may due to its higher 1,4-dioxane levels. Nevertheless, the unique flavor profiles of three asparagus cultivars have not been determined.…”

Section: Resultsmentioning

confidence: 99%

Analysis of volatile organic compounds and metabolites of three cultivars of asparagus (Asparagus officinalis L.) using E-nose, GC-IMS, and LC-MS/MS

et al. 2022

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Asparagus ( A. officinalis L.) is a perennial herb of the genus Asparagus that is rich in nutrients. This study aimed to distinguish three cultivars of asparagus (Paladin, Grace, and Jinggang red) based on their volatile organic compounds (VOCs) and metabolic profiles. VOCs in the three cultivars were separated and identified using electronic nose (E-nose) and gas chromatography–ion mobility spectrometry (GC–IMS). Differences in metabolites among the three cultivars were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). E-nose and GC-IMS showed that the VOCs in asparagus differed significantly among the three groups. E-nose result showed that purple asparagus (Jinggang red) was connected to a stronger earthy odor; green asparagus (Paladin and Grace) were shown characteristic dill flavor. Moreover, 50 VOCs were detected by using GC–IMS. Ketones and alcohols were most abundant in Paladin; methyl benzoate and dimethyl sulfide were most abundance in Grace; aldehydes and acids were most abundance in Jinggang red. Moreover, 130 and 71 different metabolites were detected in the positive and negative modes among three cultivars, such as quercetin and rutin. Functional analysis revealed that these metabolites were involved in beta-alanine metabolism and ATP-binding cassette (ABC) transporters. In summary, E-nose combined with GC-IMS and LC-MS/MS methods has good application prospects in evaluating and identifying VOCs and metabolites of different cultivars of asparagus. The identified VOCs and metabolites can provide guidelines for the development of functional asparagus products.

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“…GSH is a tripeptide produced in liver cells. It is a powerful antioxidant responsible for the detoxification process that takes place in the liver and GSH is known as the main anti-oxidant 36 , 37 . MDA is the end product of lipid peroxidation.…”

Section: Resultsmentioning

confidence: 99%

Investigation of cypermethrin toxicity in Swiss albino mice with physiological, genetic and biochemical approaches

Seven

Kültiğin²,

Çavuşoğlu

et al. 2022

Sci Rep

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In this study, cypermethrin toxicity was investigated using physiological, biochemical and cytogenetic parameters, and more than one organ and cell type was preferred to determine these effects. In this multifaceted study, the genotoxicity mechanism of cypermethrin was elucidated by molecular docking. In addition, comet assay technique was applied to detect and quantify DNA damage at the cell level. For this aim, body and organ weights, aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA), glutathione (GSH), blood urea nitrogen (BUN) and creatinine levels, mitotic index (MI), DNA fragmentation, frequency of micronucleus (MN) and chromosomal aberrations (CAs) were used as indicators of toxicity. Mice were divided into 4 groups. The control group was fed with tap water and the administration groups were orally exposed to 62.5, 125 and 250 mg/kg b.w cypermethrin for 28 days. Then, the mice were sacrificed and tissue samples were collected. Cypermethrin caused a decrease in body and organ weights, GSH levels and MI and an increase in AST, ALT, MDA, BUN, creatinine levels and the frequency of MN and CAs (break, ring, gap, acentric, etc.). Cypermethrin promoted MN formation in leukocyte, erythrocyte, buccal mucosa epithelial cells. CAs and MN formation promoted by cypermethrin have been associated with DNA-cypermethrin interactions. This interaction has been demonstrated by simulation with molecular docking method and experimentally by spectral measurements of DNA. As a result, all three doses of cypermethrin caused toxicity in different cell types. In other words, the effect of cypermethrin taken into the body was not limited to only one cell type or region. Therefore, cypermethrin is a pyrethroid insecticide that promotes multifaceted toxicity in non-target organisms.

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Genotoxic effects and molecular docking of 1,4-dioxane: combined protective effects of trans-resveratrol

Cited by 18 publications

References 54 publications

Dose-dependent toxicity profile and genotoxicity mechanism of lithium carbonate

Dose-dependent toxicity profile and genotoxicity mechanism of lithium carbonate

Analysis of volatile organic compounds and metabolites of three cultivars of asparagus (Asparagus officinalis L.) using E-nose, GC-IMS, and LC-MS/MS

Investigation of cypermethrin toxicity in Swiss albino mice with physiological, genetic and biochemical approaches

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