Genotoxic effect of two commonly used food dyes metanil yellow and carmoisine using Allium cepa L. as indicator

Khan, Ishfaq Shafi; Ali, Moonis; Hamid, Rizwan; Ganie, Showkat Ahmad

doi:10.1016/j.toxrep.2020.02.009

Cited by 66 publications

(46 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…MI is calculated to quantify the mitotic proliferation performance of the cells and estimate the cytotoxic potency of the compounds 42 . Khan et al 43 stated that the decrease in MI can be caused by halting of G2 stage of the cell cycle, failure of removing free radicals or inhibition of DNA replication. MI reduction in fungicide treated-Group 4 should suggest that diniconazole is a mitodepressive compound.…”

Section: Resultsmentioning

confidence: 99%

Preventive efficiency of Cornelian cherry (Cornus mas L.) fruit extract in diniconazole fungicide-treated Allium cepa L. roots

Macar

Yalçın

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Cornelian cherry (Cornus mas L.) is a medicinal plant with antioxidant-rich fruits. Diniconazole, a broad-spectrum fungicide, is employed extensively. The present study was designed to evaluate the preventive efficiency of C. mas fruit extract (CME) against the toxic effects of diniconazole on a model organism, Allium cepa L. For this aim, physiological, cytogenetic and biochemical parameters as well as the meristematic cell damages were investigated in A. cepa treated with diniconazole and C. mas extract. A. cepa bulbs were divided into six groups which were treated with tap water, 0.5 g/L CME, 1.0 g/L CME, 100 mg/L diniconazole, 0.5 g/L CME + 100 mg/L diniconazole and 1.0 g/L CME + 100 mg/L diniconazole, respectively. Diniconazole application caused a significant reduction in germination percentage, root elongation and total weight gain. Mitotic index decreased, while chromosomal aberrations increased following diniconazole application. Diniconazole caused significant rises in malondialdehyde content and the total activities of superoxide dismutase and catalase enzymes. The meristematic cell damages induced by diniconazole were indistinct transmission tissue, epidermis cell deformation, thickening of the cortex cell wall and flattened cell nucleus. Aqueous C. mas extracts induced a dose-dependent prevention and amelioration in all damages arisen from diniconazole application.

show abstract

Section: Resultsmentioning

confidence: 99%

Preventive efficiency of Cornelian cherry (Cornus mas L.) fruit extract in diniconazole fungicide-treated Allium cepa L. roots

Macar

Yalçın

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Toxic activity is assessed when root growth is inhibited (Leite et al, 2015;Bolonhesi and Lopes, 2018), while cytotoxic activity is verified when mitotic index is inhibited (Mercado and Caleño, 2020;Mercado and Bayona, 2020). Genotoxicity is assessed through the frequency of chromosomal aberrations (Khan et al, 2020), while mutagenicity is evaluated through the micronucleus frequency (Silva et al, 2013). Thus, the A. cepa system is a key in vivo model in the evaluation of natural and synthetic substances, capable of identifying damage at the cellular level (Leite et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Research Article Toxicogenetic potential of Mimosa pigra (Fabaceae) infusion in Allium cepa meristematic cells

Araújo¹,

Santos²,

Barros-Filho³

et al. 2020

Genet. Mol. Res.

View full text Add to dashboard Cite

Mimosa pigra is a plant commonly used for medicinal purposes in the treatment of several diseases. However, studies regarding its toxicological properties are scarce. We evaluated the toxic, cytotoxic, genotoxic and mutagenic activities of leaf and stem infusions of M. pigra collected in the region of Delta do Parnaíba, Piauí, Brazil, through an in vivo Allium cepa test. Three concentrations of leaf and stem treatments were used (leaf treatment 1 and stem treatment 1 = 0.771 g/L; leaf treatment 2 and stem treatment 2 = 1.542 g/L; leaf treatment 3 and stem treatment 3 = 3.084 g/L), in addition to the negative controls (water) and positive controls (copper sulfate-1.2 mg/mL-A. cepa test). All the treatments were toxic, causing significant root growth inhibition. Leaf treatment 1, leaf treatment 3 and stem treatment 1 showed significant inhibition of the mitotic index. Leaf treatment 2, stem treatment 1 and stem treatment 3 showed significant genotoxic activity based on the frequency of chromosomal aberrations. The tested concentrations did not provoke significant mutagenic activity ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 19 (3): gmr18588 M.S. Araújo et al. 2 when compared to the negative control. We suggest that further studies with other biological systems are needed to evaluate the safetyness of M. pigra infusions for therapeutic purposes.

show abstract

“…The delocalization of aryl gives vivid colors such as red, orange, and yellow. It is usually produced by an electrophilic substitution reaction of an aryl diazotization with acetoacetic amide or aromatic amines (Clarke and Kirner, 1941), which are well documented carcinogenic and mutagenic agents (Khan et al, 2020).Several studies have been conducted on the nature and characterization of azo dyes which presented a higher level of cytotoxicity on plants cells, severe allergic reactions in humans, and even cancer in lab-tested animals than any other synthetic FCAs (Rangan and Barcelous, 2009;Micic et al, 2014;Kaur et al, Abstract | The frequent use of meal and beverages having food color additives (FCAs) may cause mutation in humans' proto-oncogenes which leads to carcinogenicity. The current findings aimed to evaluate the genotoxic impact of widely used azo FCAs on the cell cycle by using onion as a model plant.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

“…2019). The carmoisine or azorubine, metanil yellow, sunset yellow, tartrazine, and brilliant blue CFC induced number of mitotic aberrations in onion's meristematic cells such as micronuclei, binucleate cell, fragmentation, c-mitosis, bridge anaphase, laggard, forwarded, and stickiness at meta and anaphase of chromosomes (Kaur et al, 2019;Khan et al, 2020). Likewise, the allura red, sunset yellow, tartrazine, and fast green azo dye causes abdominal pain, headache, nausea, eczema, eye, and skin rashes and swelling as well as asthma in children (Freitas, 2012;Oliveira et al, 2013).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Geno-Toxic Appraisal of Widely Used Food Color Additives on Model Plant Allium cepa Root Tip Cells

Farheen¹,

Mansoor²,

Abid³

2021

JIS

View full text Add to dashboard Cite

Azo dyes are consisted of nitrogen and carbon atom having R-N= N-R' functional group in which Rs is usually aryl. The delocalization of aryl gives vivid colors such as red, orange, and yellow. It is usually produced by an electrophilic substitution reaction of an aryl diazotization with acetoacetic amide or aromatic amines (Clarke and Kirner, 1941), which are well documented carcinogenic and mutagenic agents (Khan et al., 2020).Several studies have been conducted on the nature and characterization of azo dyes which presented a higher level of cytotoxicity on plants cells, severe allergic reactions in humans, and even cancer in lab-tested animals than any other synthetic FCAs (Rangan and Barcelous, 2009;Micic et al., 2014;Kaur et al., Abstract | The frequent use of meal and beverages having food color additives (FCAs) may cause mutation in humans' proto-oncogenes which leads to carcinogenicity. The current findings aimed to evaluate the genotoxic impact of widely used azo FCAs on the cell cycle by using onion as a model plant. The study was designed in a complete randomized design where the grown onion roots were exposed to 0%, 0.001%, 0.01%, 0.1%, and 1% concentration of FCA for 120 hours for macroscopic and 36 h for microscopic evaluation. The onion root tips morpho-physiology was severely affected as the concentration of FCAs increased. The macroscopic analysis manifested that allura red had 95% broken-ended extremely thin and transparent root tips at 1% concentration. In the microscopic analysis, FCAs induced eight different kinds of chromosomal aberrations in which allura red significantly induced the highest frequencies of abnormal prophase, sticky metaphase, c-mitosis, sticky anaphase, and laggard anaphase at each concentration. While, dislocated metaphase, forwarded anaphase, and bridge anaphase were only found in sunset yellow, tartrazine, and fast green, respectively. The use of evaluated FCAs especially allura red in foodstuff, cosmetics, and skincare products can cause cancer in humans.

show abstract

Genotoxic effect of two commonly used food dyes metanil yellow and carmoisine using Allium cepa L. as indicator

Cited by 66 publications

References 31 publications

Preventive efficiency of Cornelian cherry (Cornus mas L.) fruit extract in diniconazole fungicide-treated Allium cepa L. roots

Preventive efficiency of Cornelian cherry (Cornus mas L.) fruit extract in diniconazole fungicide-treated Allium cepa L. roots

Research Article Toxicogenetic potential of Mimosa pigra (Fabaceae) infusion in Allium cepa meristematic cells

Geno-Toxic Appraisal of Widely Used Food Color Additives on Model Plant Allium cepa Root Tip Cells

Contact Info

Product

Resources

About