Genotoxic and mutagenic activity of environmental air samples in Flanders, Belgium

Four, V.A Du; Larebeke, N. Van; Janssen, Colin R.

doi:10.1016/j.mrgentox.2003.12.002

Cited by 34 publications

(14 citation statements)

References 34 publications

(50 reference statements)

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“…The seasonal differences in the total PAH levels were not as pronounced as that for the inorganic compounds found in PM: the ranges were 10-24 and 9-16 ng/m 3 in cold-dry and in the warm-dry seasons, respectively. The average PAH concentration (18.9 ng/m 3 ) was comparable to the 0.3-51.9 ng/m 3 range reported for other regions, such as Santiago, Chile, and Flanders, Belgium [Arrieta et al, 2003;Du Four et al, 2004], but it was higher than the PAH levels reported for rural areas and for some of the US cities (ranging from 0-0.4 ng/m 3 ) [WHO, 2000]. High proportions of carcinogenic PAHs (53.3%-73.3%) were found for the PAHs detected in the PM organic extracts, and these PAHs may be partially responsible for the observed genotoxicity.…”

Section: Chemical Characteristics Of Pmsupporting

confidence: 79%

Effect of chemical composition on the induction of DNA damage by urban airborne particulate matter

Gutiérrez-Castillo

Roubicek²,

Cebrián-García

et al. 2005

Environ and Mol Mutagen

109

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Airborne particulate matter (PM) contains a large number of genotoxic substances capable of endangering human health. In the present study, we have investigated the ability of chemically characterized water-soluble and organic-soluble fractions of two particle sizes (PM2.5 and PM10) from different regions of Mexico City to induce DNA damage in a human lung epithelial cell line. We also evaluated associations between the physicochemical parameters of the PM and its genotoxicity. The airborne particulate samples were collected from four regions of the city; a HiVol air sampler was used to collect PM10 on glass fiber filters and a tapered element oscillating system coupled to an automatic cartridge collection unit was used to collect PM2.5 on teflon filters. PM mass was determined by gravimetric analysis of the filters. Filters containing PM2.5 and one section of each PM10 filter were agitated either with deionized water to extract water-soluble compound, or with dichloromethane to prepare organic-soluble compounds. The chemical composition of the extracts was determined by ion and gas chromatography and atomic adsorption spectroscopy. A549 human type II alveolar epithelial cells were exposed to different concentrations of the PM2.5 and PM10 extracts, and alkaline single cell gel electrophoresis or the Comet assay was performed to measure DNA damage and repair. These analyses indicated that soluble transition metals and the organic-soluble PM fractions are crucial factors in the DNA damage induced by PM. PM composition was more important than PM mass for producing genotoxicity. The results of this study showed that the constituents of the water-soluble PM extract are more likely to induce DNA damage than the organic compounds.

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Section: Chemical Characteristics Of Pmsupporting

confidence: 79%

Effect of chemical composition on the induction of DNA damage by urban airborne particulate matter

Gutiérrez-Castillo

Roubicek²,

Cebrián-García

et al. 2005

Environ and Mol Mutagen

109

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“…Also it remains possible that both intensive agriculture (pesticides, fertilizers) and some industrial activities at a distance of about 15 km were involved. In 2000–2001, episodes of air pollution with polycyclic aromatic hydrocarbons and high mutagenic activity were observed in Peer ( Du Four et al 2004 ). These data and also the data from an ongoing Flemish biomonitoring program involving other rural areas ( Milieu en Gezondheid 2006 ) indicate that pollution is widespread in Flanders and suggest that exposure to pollutants may be quite homogeneous in some developed Western nations.…”

Section: Discussionmentioning

confidence: 99%

Differences in Tumor-Associated Protein Levels among Middle-Age Flemish Women in Association with Area of Residence and Exposure to Pollutants

Larebeke¹,

Bracke

Nelen

et al. 2006

Environ Health Perspect

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We measured tumor-associated proteins (TAPs) and pollutants in blood, serum, and urine of 200 nonsmoking women 50–65 years of age, residing in the rural municipality of Peer or in Hoboken or Wilrijk, industrial suburbs of Antwerp, Belgium. Persons with occupational exposures or commuting to other towns were excluded. Residents from Hoboken had significantly higher levels of blood lead and serum zinc and polychlorinated biphenyls. Surprisingly, residents of Peer had significantly higher levels of serum cadmium, dioxin-like activity in blood fat, and urinary 1-hydroxypyrene. For 5 of the 12 TAPs assessed in this study, we observed significant differences in serum levels among residents of the three municipalities after adjusting for personal or lifestyle parameters. Although we found levels of internal exposure to pollutants to be quite homogeneous in Flanders, we found significantly higher levels of TAPs only in the industrial suburbs. In multiple regression with all 29 available personal, lifestyle, and internal exposure parameters, blood lead levels showed a positive association with serum levels of anti-p53, carcino-embryonic antigen (CEA), and tissue polypeptide–specific antigen (TPS) and with an index for mean TAP level (Itap); dioxin-like activity in serum and serum copper showed a positive association with serum CA 125 (cancer antigen 125); and serum zinc showed a positive association with serum levels of c-erbB-2 ectodomain and TPS. An index of internal exposure showed a positive association with serum levels of both CEA and anti-p53 and with Itap. This study provides some evidence that levels of internal exposure such as those present in Flanders, in particular concerning lead, are indeed associated with biologic effects.

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“…The environmental conditions during these periods were characterized by low temperatures and low relative humidity, which may lead to a higher molecular weight of the atmospheric pollutant. Similarly, studies report a higher mutagenic potential in colder months in European countries (Binkov a et al, 2003;Du Four et al, 2004;Piekarska et al, 2009Piekarska et al, , 2011, North and South America (Brown et al, 2006;Cavanagh et al, 2009) and New Zealand (T€ or€ ok et al, 1989;Müller et al, 2001).…”

Section: Mcnmentioning

confidence: 94%

Evaluation of vehicular pollution using the TRAD-MCN mutagenic bioassay with Tradescantia pallida (Commelinaceae)

Rocha

Candido

Pereira

et al. 2018

Environmental Pollution

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Biomonitoring is one of the tools used to assess the mutagenic potential of the atmosphere. In this study, the mutagenicity of Tradescantia pallida, a species of plant largely present in urban environments, was investigated. The objectives of this study was to estimate the mutagenic potential of vehicular flow through the TRAD-MCN bioassay in cities located at different altitudes in the southwest mesoregion of Mato Grosso do Sul, Brazil, to infer possible abiotic agents that may contribute to the effects of atmospheric pollutants, and finally to map the cities with greater risks to the health of the local population. To achieve these objectives, the Tradescantia-micronucleus test was performed on young buds of T. pallida collected between August 2015 and August 2016 in nine cities of Mato Grosso do Sul. These buds were exposed to traffic flows of various intensities. The data collected consisted of measurements of meteorological parameters and vehicular traffic counts for each city. The variables considered were: mean ambient temperature; micronuclei frequency; vehicular flow; altitude; relative humidity; pluviosity. The application of the Trad-MCN bioassay, with the consideration of environmental variables and altitudes, and the use of the Kernel interpolation technique, allowed us to map the areas with significant pollution risks to the population. The highest frequency of exposure to mutagens occurred in the cities with the highest vehicular traffic intensity. The average ambient temperature failed to show a linear association with the frequency of the micronuclei in the samples analyzed (r = 0.11). A positive correlation was observed between micronuclei frequency and vehicular flow, (r = 0.67; p ≤ 0.001%) and between micronuclei frequency and altitude (r = 0.24; p ≤ 0.05). A negative correlation was found between relative humidity and micronuclei frequency (r = -0.19; p ≤ 0.05%). Thus, higher micronuclei frequency tended to be present in locations with low relative humidity and high altitudes and vehicular flow.

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Genotoxic and mutagenic activity of environmental air samples in Flanders, Belgium

Cited by 34 publications

References 34 publications

Effect of chemical composition on the induction of DNA damage by urban airborne particulate matter

Effect of chemical composition on the induction of DNA damage by urban airborne particulate matter

Differences in Tumor-Associated Protein Levels among Middle-Age Flemish Women in Association with Area of Residence and Exposure to Pollutants

Evaluation of vehicular pollution using the TRAD-MCN mutagenic bioassay with Tradescantia pallida (Commelinaceae)

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