Bagasse is an underutilized agro-industrial residue with great potential as raw material for the production of cellulose nanofibrils (CNF) for a range of applications. In this study, we have assessed the suitability of bagasse for production of CNF for three-dimensional (3D) printing. Firstly, pulp fibers were obtained from the bagasse raw material using two fractionation methods, i.e. soda, and hydrothermal treatment combined with soda.Secondly, the pulp fibers were pre-treated by TEMPO-mediated oxidation using two levels of oxidation for comparison purposes. Finally, the CNFs were characterized in detail and assessed as inks for 3D printing. The results show that CNF produced from fibers obtained by hydrothermal and soda pulping were less nanofibrillated than the corresponding material