Genotoxic and cytotoxic effects of ZnO nanoparticles for  Dunaliella tertiolecta  and comparison with SiO 2  and TiO 2  effects at population growth inhibition levels

Schiavo, Simona; Oliviero, Maria; Miglietta, Maria Lucia; Rametta, Gabriella; Manzo, Sonia

doi:10.1016/j.scitotenv.2016.01.135

Cited by 105 publications

(28 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The gel is then analysed for the amount of fluorescence in the head and tail and the tail length [62][63][64]. Comet assay was used to assess the toxicity of zinc oxide nanoparticles at 25 mg Zn/L on D. tertiolecta which resulted in 55% nuclei damage [65]. In another investigation, comet assay was used to measure the toxicity imposed by SiO 2 nanoparticles on D. tertiolecta at 125 mg/L, which resulted in increasing genotoxic effects after 72 h [66].…”

Section: Apoptosis Assaymentioning

confidence: 99%

In vitro and in vivo toxicity assessment of nanoparticles

2017

View full text Add to dashboard Cite

Nanotechnology has revolutionized gene therapy, diagnostics and environmental remediation. Their bulk production, uses and disposal have posed threat to the environment. With the appearance of these nanoparticles in the environment, their toxicity assessment is an immediate concern. This review is an attempt to summarize the major techniques used in cytotoxity determination. The review also presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles including carbon nanotubes, gold nanoparticles, silver nanoparticles, quantum dots, fullerenes, aluminium nanoparticles, zinc nanoparticles, iron nanoparticles, titanium nanoparticles and silica nanoparticles. It discusses the in vitro and in vivo toxological effects of nanoparticles on bacteria, microalgae, zebrafish, crustacean, fish, rat, mouse, pig, guinea pig, human cell lines and human. It also discusses toxological effects on organs such as liver, kidney, spleen, sperm, neural tissues, liver lysosomes, spleen macrophages, glioblastoma cells, hematoma cells and various mammalian cell lines. It provides information about the effects of nanoparticles on the gene-expression, growth and reproduction of the organisms.

show abstract

Section: Apoptosis Assaymentioning

confidence: 99%

In vitro and in vivo toxicity assessment of nanoparticles

2017

View full text Add to dashboard Cite

show abstract

“…The third mechanism is related with the shape, size and surface characteristics of nTiO 2 , which alter its availability to the plants (Zhang et al, 2015). On the other hand, higher concentrations of nTiO 2 may cause cyto-toxicity and geno-toxicity in plants (Mattiello et al, 2015;Schiavo et al, 2016). Thus, considering its multifaceted effects, precise optimizations of doses and modes of nTiO 2 applications are required, before its applications could be recommended at a wider scale on crop plants.…”

Section: Introductionmentioning

confidence: 99%

Titanium Dioxide Nanoparticles Improve Growth and Enhance Tolerance of Broad Bean Plants under Saline Soil Conditions

et al. 2017

View full text Add to dashboard Cite

Soil salinity is established as one of the major environmental problems, decreasing crop productivity worldwide, thereby threatening sustainable agriculture. In the present study, we evaluated the effects of titanium dioxide nanoparticles (nTiO2) for ameliorating soil salinity in broad bean, an important leguminous crop. As nTiO2 is known to have pro‐oxidant and antioxidant properties, the effects of three different nTiO2 concentrations (0·01%, 0·02% and 0·03%) were compared with respect to plant growth and stress responses. The 0·01% nTiO2 application significantly increased shoot length, leaf area and root dry weight of plants under normal conditions. These growth‐promoting effects were simultaneous with increased levels of chlorophyll b, soluble sugars and proline and enhanced activities of antioxidant enzymes. Under saline soil conditions, although proline level and enzymatic antioxidant activities were increased, plant growth significantly reduced. The 0·01% nTiO2 supplementation significantly increased the activities of enzymatic antioxidants and levels of soluble sugars, amino acids and proline in salt‐affected plants versus plants subjected to salinity alone. Thus, the increased antioxidant enzyme activities contributed to the observed reduction in hydrogen peroxide and malondialdehyde contents, while enhanced levels of proline and other metabolites contributed to osmoprotection, collectively resulting in significant plant growth improvement under salinity. Furthermore, nTiO2‐mediated positive effects were concentration dependent with 0·01% nTiO2 being the most effective, whereas 0·02% showed an intermediate response and 0·03% was almost ineffective under both control and saline soil conditions. Our findings provide a foundation for nTiO2 application in improving growth of plants cultivated on naturally contaminated saline soils. Copyright © 2017 John Wiley & Sons, Ltd.

show abstract

“…No obstante, al igual que con otras NP, las NP de ZnO, SiO 2 y TiO 2 han sido señaladas como potenciales agentes perjudiciales para la salud humana y el medioambiente (8) . Estudios previos para estas tres NP han reportado su efecto citotóxico en los fibroblastos pulmonares humanos (9) , , un efecto inflamatorio en las células intestinales mediante la producción elevada de especies reactivas de oxígeno (ROS) (6) así como también efecto genotóxico y citotóxico en modelos vegetales (10) . Por estas evidencias, los autores recomiendan una extrema atención para el uso seguro de estos nanomateriales (9) .…”

Section: Introductionunclassified

Efecto de las nanopartículas industriales TiO2, SiO2 y ZnO sobre la viabilidad celular y expresión génica en médula osea roja de Mus musculus

Zarria-Romero

Osorio

Pino

et al. 2017

Rev Peru Med Exp Salud Publica

View full text Add to dashboard Cite

RESUMENObjetivos. Evaluar el efecto de las nanopartículas de ZnO, TiO 2 y SiO 2 sobre la viabilidad celular y la expresión génica de las interleuquinas 7 y 3 y del factor estimulante de colonias de granulocito -macrófago (GM-CSF) en Mus musculus. Materiales y métodos. Se extrajo médula ósea roja de cinco roedores (Balb/c) para el estudio de viabilidad celular mediante la prueba de MTT. Por otro lado, grupos cinco roedores fueron inoculados vía intraperitoneal con dosis de 0,5; 1; 2,5; 5 y 10 mg/kg de nanopartículas de ZnO y SiO 2 y de 5; 10; 15; 20 y 25 mg/kg de nanopartículas deTiO 2 , 30 h después, se obtuvo el ARN a partir de la médula ósea roja para los análisis de expresión génica empleando las técnicas de PCR y RT-PCR cuantitativa. Resultados. Las nanopartículas de ZnO y SiO 2 redujeron la viabilidad celular de una manera dosis-dependiente en un 37 y 26%, respectivamente, a partir de una dosis de 1 mg/kg. En cuanto al efecto sobre la expresión génica, a las dosis 5 y 10 mg/kg, las nanopartículas de TiO 2 redujeron en mayor porcentaje la expresión de las interleuquinas 7 y 3 (55,3 y 70,2% respectivamente), con respecto a la expresión del GM-CSF, el mayor porcentaje de reducción lo produjo las nanopartículas de SiO 2 (91%). Las nanopartículas de ZnO redujeron a partir de las dosis de 20 y 25 mg/kg. Conclusiones. Las nanopartículas de ZnO, SiO 2 y TiO 2 alteran la viabilidad celular y la expresión génica en la médula ósea de ratón. Palabras clave: Nanopartículas; Interleucinas; Expresión génica; Mus musculus (fuente: DeCS BIREME). EFFECT OF THE INDUSTRIAL NANOPARTICLES TiO 2 , SiO 2 and ZnO ON CELL VIABILITY AND GENE EXPRESSION IN RED BONE MARROW OF Mus musculus ABSTRACT Objectives.To evaluate the effect of ZnO, TiO 2 and SiO 2 nanoparticles on cell viability and expression of the interleukin 7, interleukin 3, and granulocyte-macrophage colony stimulating factor (GM-CSF) genes in Mus musculus. Materials and methods. Red bone marrow was extracted from five Balb/c mice for the analysis of cell viability using the MTT test. The mice were divided into two groups of five each: one group was inoculated intraperitoneally with 0.5, 1.0, 2.5, 5.0, and 10 mg/kg of ZnO and SiO 2 nanoparticles, respectively, and the other group was inoculated with 5.0, 10.0, 15.0, 20.0, and 25 mg/kg of TiO 2 nanoparticles, respectively. Thirty hours later, RNA was extracted from the red bone marrow of the mice in both groups for gene expression analysis using quantitative PCR and RT-PCR. Results. ZnO and SiO 2 nanoparticles reduced cell viability in a dose-dependent manner by 37% and 26%, respectively, starting at a dose of 1 mg/kg. TiO 2 nanoparticles at 5 mg/kg and 10 mg/kg reduced the gene expression of interleukins 7 and 3 by 55.3% and 70.2%, respectively, and SiO 2 nanoparticles caused the greatest decrease (91%) in the expression of GM-CSF. ZnO nanoparticles reduced the expression of GM-CSF starting at doses of 20 mg/kg and 25 mg/kg. Conclusions: ZnO, SiO 2 and TiO 2 nanoparticles affect cell viability and gene expression in the mo...

show abstract

Genotoxic and cytotoxic effects of ZnO nanoparticles for Dunaliella tertiolecta and comparison with SiO 2 and TiO 2 effects at population growth inhibition levels

Cited by 105 publications

References 61 publications

In vitro and in vivo toxicity assessment of nanoparticles

In vitro and in vivo toxicity assessment of nanoparticles

Titanium Dioxide Nanoparticles Improve Growth and Enhance Tolerance of Broad Bean Plants under Saline Soil Conditions

Efecto de las nanopartículas industriales TiO2, SiO2 y ZnO sobre la viabilidad celular y expresión génica en médula osea roja de Mus musculus

Contact Info

Product

Resources

About