Genotipificación de los factores de virulencia vacA y cagA de Helicobacter pylori en individuos de dos regiones de Colombia con riesgo opuesto de cáncer gástrico

Trujillo, Esperanza; Martínez, Teresa; Bravo, María Mercedes

doi:10.7705/biomedica.v34i4.2273

Cited by 13 publications

(7 citation statements)

References 16 publications

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“…The genotyping of virulence genes showed that in our population the frequency of the cagA gene was lower (59.5%) than that reported in previous investigations in Colombian mountain regions 33,48,64,65 and other Latin American countries, with 67 to 80% reported in patients with premalignant lesions. 34,66−68 The presence of the cagE gene has been suggested as a better marker than the cagA gene about the integrity of the Cag Pathogenicity Island (cag PAI), and it has been observed with a frequency greater than 80% in patients with gastrointestinal diseases in other countries.…”

Section: Discussioncontrasting

confidence: 83%

Infection with Helicobacter Pylori Presenting the vacA s2m2 haplotype is Strongly Associated with Protection Against Gastric Cancer

Guevara-Tique¹,

Castro-Valencia²,

Olaya³

et al. 2021

Preprint

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Background: Infection with Helicobacter pylori is recognized as the main risk factor for gastric cancer (GC); the clinical outcome of this infection is variable and partially depends on the virulence of the infective strain. This study characterizes H. pylori virulence genes in patients with diverse gastric lesions, from preneoplasia to GC, from a South American region with high GC mortality rates.Methods: We studied the virulence profiles of H. pylori strains to colonize the antrum of 318 patients with non-atrophic gastritis (NAG), 58 patients with preneoplastic lesions (PN), and 90 with GC from Ibagué, Colombia. The presence of 16S rDNA, the cagA and cagE genes, and the vacA s1, s2, m1, and m2 alleles were determined by PCR.Results: H. pylori infection was detected in 44% of all patients, 41.2% in NAG, 43.1% in PN and 54.4% of GC patients (p= 0.0813). cagA and cagE genes were significantly more frequent in and GC than in NAG (p= <.0001). The vacA s1m1 haplotype was significantly more frequent in PN (68%) and GC (65.3%) than in NAG (37.4%). The frequency of vacA s2m2 haplotype decreased significantly from NAG (42.7%) to PN (12%) and this to GC (4.1%). A total of 23 different genotypes were identified, with cagA+/cagE+/vacA s1m1 (84/205) as the more frequent in PN and GC and cagA-/cagE-/vacA s2m2 in NAG (49/205).Conclusions: In the population studied, vacA s2m2 was identified as a significant marker for protection against PN and GC, and genotype cagA+/cagE+/vacA s1m1 as a marker for increased GC risk. We also found that patients with PN and GC had a higher frequency of cagA+/cagE+/vacA s1m1 H. pylori strains known to be aggressive.

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Section: Discussioncontrasting

confidence: 83%

Infection with Helicobacter Pylori Presenting the vacA s2m2 haplotype is Strongly Associated with Protection Against Gastric Cancer

Guevara-Tique¹,

Castro-Valencia²,

Olaya³

et al. 2021

Preprint

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“…In our country we have found that H. pylori infects over 77% of the adult population (Trujillo et al 2014), despite this high prevalence of the infection, gastric cancer is present in a minimum percentage of the infected population. Therefore, it is important to identify the factors that determine that only a subgroup of infected individuals develops a serious gastroduodenal disease while the majority remain carriers.…”

Section: Discussionmentioning

confidence: 73%

El islote de patogenicidad cag de Helicobacter pylori y su asociación con lesiones preneoplásicas y cáncer gástrico

Liévano¹,

Gama²,

Bravo³

2018

Rev. U.D.C.A Act. & Div. Cient.

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Gastric cancer is one of the main causes of death by cancer in the world and the infection with Helicobacter pylori is one of the main risk factors associated with its appearance. Helicobacter pylori is a bacterium that colonizes the gastric mucosa, infecting about half of the world´s population. The pathological effects caused by infections with H. pylori greatly depend on an IV type secretion system encoded in the cag pathogenicity island (cagPAI). In this review, we describe the composition of the cagPAI, the alterations of cellular signaling pathways mediated by cagPAI which regulate oncogenic cellular responses that may increase the risk of malignant transformation associated with the infection and the importance of polymorphisms in cagPAI genes as potential markers of progression to gastric cancer.

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“…A study conducted in northeastern Brazil reported that a high prevalence of the combination of H. pylori cagA and vacA s1m1 genes was associated with a more severe gastric condition (Cavalcante et al, 2012). Other studies conducted in Venezuela, Cuba, and Colombia have also demonstrated an association between the combination of cagA and vacA and the risk of developing gastric diseases (Ortiz-Princz et al, 2010;Takahashi et al, 2013;Trujillo et al, 2014;Sallas et al, 2017). In the Moroccan population, cagA-positive strains were also the most prevalent type and were most highly associated with the vacA allelic subtype, s2m2 (Boukhris et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested that the detection of dupA is not sufficient to assess its role in gastric pathologies. In addition to its presence, it is necessary to evaluate the size of the gene product, as shorter forms of the protein are inactive (Trujillo et al, 2014). Analyses of the whole dupA gene cluster may provide a better understanding of its role in the pathogenesis of H. pylori infection (Jung et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Research Article Prevalence of Helicobacter pylori cagA, dupA, and vacA genotypes and their association with the severity of gastropathies in patients with dyspepsia

Silva¹,

Oliveira²,

Gama³

et al. 2021

Genet. Mol. Res.

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Helicobacter pylori is a gram-negative bacterium associated with the development of severe gastric pathologies, such as atrophy, metaplasia, and gastric adenocarcinoma. This microorganism is considered a class I carcinogen by the International Agency for Research on Cancer. The virulence genes in the strain causing infection influence the clinical outcome and can be used as specific markers for the severity of gastric diseases. We evaluated H. pylori infection and associations of cagA, vacA and dupA virulence genes with gastric pathologies. Antral and gastric body biopsies of 117 patients with dyspepsia were analyzed by histological and molecular techniques. Screening for H. pylori infection was ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 20 (3): gmr18883 L.L.L. Silva et al. 2performed using the hpx gene (16S rRNA). Positive samples were evaluated for vacA, cagA, and dupA virulence genes. The clinical outcomes presented by the patients were stratified according to severity, being considered severe lesions atrophy, metaplasia and adenocarcinoma. Gastritis, duodenitis, esophagitis, ulcer, and xanthelasma were considered non-severe pathologies. The prevalence of infection was 64.1%, with a high frequency of strains positive for cagA (80.0%), dupA (70.7%), and vacA (56.0%). The cagA gene was detected in all isolates from patients with severe pathologies, whereas the vacA gene was not detected in this group. Simultaneous detection of the three genes was observed in 14.3% and 35.8% of the isolates from individuals with severe and non-severe pathologies, respectively. Furthermore, fewer virulence genes were detected in isolates from patients with severe disease (0.7) than in isolates from non-severe cases (1.4). Patients with severe diseases had a higher mean age and greater number of gastric diseases than patients with non-severe pathologies. The circulating H. pylori strains in the Brazilian Midwest exhibit high heterogeneity in the frequencies of virulence genes. Individual virulence factors and their combinations may influence clinical outcomes.

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Genotipificación de los factores de virulencia vacA y cagA de Helicobacter pylori en individuos de dos regiones de Colombia con riesgo opuesto de cáncer gástrico

Cited by 13 publications

References 16 publications

Infection with Helicobacter Pylori Presenting the vacA s2m2 haplotype is Strongly Associated with Protection Against Gastric Cancer

Infection with Helicobacter Pylori Presenting the vacA s2m2 haplotype is Strongly Associated with Protection Against Gastric Cancer

El islote de patogenicidad cag de Helicobacter pylori y su asociación con lesiones preneoplásicas y cáncer gástrico

Research Article Prevalence of <i>Helicobacter</i> <i>pylori</i> <i>cagA</i>, <i>dupA</i>, and <i>vacA</i> genotypes and their association with the severity of gastropathies in patients with dyspepsia

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