Genosensor for SARS Virus Detection Based on Gold Nanostructured Screen‐Printed Carbon Electrodes

Abstract

A hybridization-based genosensor is designed on a disposable gold nanostructured screen-printed carbon electrode. Gold nanoparticles which are formed in situ by applying a constant current intensity during a fixed time, acts as an immobilization and transduction surface. The parameters affecting the methodology are studied, with special attention being placed on sensibility. Immobilization takes place through thiol-gold interaction in a relatively fast way, and the genosensor response is found to be linearly to the biotinylated target concentration between 2.5 and 50 pmol/ L; a detection limit of 2.5 pmol/L is calculated, and a sensitivity of 1.76 mA/pmol L À1 is achieved. These results improve significantly those described in literature, thus demonstrating the advantages offered by the use of goldnanostructured surfaces.

Ternary Monolayer Interfaces for Ultrasensitive and Direct Bioelectronic Detection of Nucleic Acids in Complex Matrices

“…These important advantages of SPEs make them extremely attractive for diverse clinical applications, in both centralized and decentralized settings [45,46]. The use of SPEs for the development of DNA hybridization biosensors has been widely reported [47][48][49][50]. Considerable efforts have been devoted for enhancing the performance, particularly the sensitivity and selectivity of DNA hybridization biosensors based on SPEs.…”

Genosensor for detection of four pneumoniae bacteria using gold nanostructured screen-printed carbon electrodes as transducers

“…The procedure to perform the enzymatic reaction of alkaline phosphatase has been previously optimized [19], and used in more recent works [23]. In this procedure the substrate (3-indoxyl phosphate (3-IP)), produces a compound able to reduce silver ions in solution into a metallic deposit, which is located where the enzymatic label AP is attached.…”

“…Although thick gold substrates are reported in the literature for enzymatic DNA detection (screen-printed gold electrodes [24], 2m thick film gold electrodes [25], or gold disk electrodes [26]), gold nanoparticles have been unusually used as electrochemical transducers [23], in despite of their widespread use as DNA labels due to the electrochemical properties of gold nanoparticles [27].…”

“…In this paper, a DNA hybridization assay with enzymatic electrochemical detection was carried out on a gold nanostructured screen-printed carbon electrode (SPCnAuE), which allows working with small volumes, and has been proved to obtain greater sensitivity than that obtained with non-nanostructured surfaces [23]. Although thick gold substrates are reported in the literature for enzymatic DNA detection (screen-printed gold electrodes [24], 2m thick film gold electrodes [25], or gold disk electrodes [26]), gold nanoparticles have been unusually used as electrochemical transducers [23], in despite of their widespread use as DNA labels due to the electrochemical properties of gold nanoparticles [27].…”

Nanohybrid Materials as Transducer Surfaces for Electrochemical Sensing Applications

“…For instance, Martínez-Paredes and colleagues [19] proposed an electrochemical method to generate gold nanostructured screen-printed carbon electrodes. The developed electrochemical surface was applied to the detection of lead underpotential deposition and to the construction of geno- [20] and immunosensors [21]. NPAus works as an excellent adsorbent support, solving carbon limitations and enhancing biocompatibility [14].…”