Genosensor for detection of four pneumoniae bacteria using gold nanostructured screen-printed carbon electrodes as transducers

Martínez-Paredes, Graciela; González‐García, María Begoña; Costa-Garcı́a, Agustı́n

doi:10.1016/j.snb.2010.06.064

Cited by 48 publications

(15 citation statements)

References 30 publications

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“…Among voltammetric methods, anodic stripping voltammetry (ASV) represents one of the most promising compared to any other electrochemical techniques, able to increase of sensitivity of others 2 or 3 orders of magnitude, reaching limit of detection (LOD) in the range of 10 −9 to 10 −10 M. In ASV, a metal is deposited on the surface of the electrodes during a preconcentration step, and it is then stripped applying an oxidizing potential. In addition to heavy metals quantification, interesting applications of ASV for proteins [7], biomolecules [11], Deoxyribonucleic acid (DNA) sequences [12] have been investigated. Labeling proteins with specific catalyzing enzymes, proteins concentration can be indirectly quantified as proportional to the amount of metal deposited by measuring current peak metal during the stripping step.…”

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confidence: 99%

Wireless Point-of-Care Platform With Screen-Printed Sensors for Biomarkers Detection

Tonello

Abate

Borghetti

et al. 2017

IEEE Trans. Instrum. Meas.

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Abstract-Measurement systems for early and reliable detection of degenerative diseases, such as Alzheimer's disease (AD), are extremely important in clinical diagnosis. Among these, biochemical assays represent a commonly used method to distinguish patients from healthy population thanks to the sensitive recognition of specific biomarkers in biological fluids. In order to overcome actual limitations of these techniques in term of cost, standardization, and sensitivity, this study aimed to realize a low-cost highly sensitive portable point-of-care (PoC) testing system based on screen-printed electrochemical sensors. The development of the platform specifically included both the design of the sensing probe and the electronic circuit devoted to condition and acquires the transduced electric signal. The designed circuit was implemented in a printed circuit board and interfaced to a wireless system based on bluetooth data transmission in order to improve the portability of the proposed solution. Preliminary results were obtained by using controlled concentrations of electrolytic solutions and calibrating the sensors for antibodies and for a well-known protein (i.e., interleukin 8) quantified by anodic stripping voltammetry (ASV). Findings from ASV measurements showed a sensitivity of 38 µA/(ng/ml) with a tested range from 1.25 to 20 ng/ml, with a limit of detection of 2 ng/ml. Further investigation will include the validation of this PoC device by testing the concentration of a specific p53 protein isoform, which was recently identified to early correlate to AD development.

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confidence: 99%

Wireless Point-of-Care Platform With Screen-Printed Sensors for Biomarkers Detection

Tonello

Abate

Borghetti

et al. 2017

IEEE Trans. Instrum. Meas.

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“…The use of gold nanoparticles as the immobilization and transduction surface enhanced the sensitivity and detection limit of the genosensor developed by Martinez-Paredes, for the detection of bacteria associated with respiratory tract infections such as Mycoplasma pneumoniae, Legionella pneumophila, Chlamydophila pneumoniae, andStreptococcus pneumonia. 113 Oligonucleotide molecules were adsorbed onto the screen-printed electrodes using mercaptohexanol as a spacer in the detection of enterobacteriaceae lac Z, which is an indicator of faecal contamination. 114 A disposable magnetic genosensor was also proposed by the same group of researchers for the analysis of Escherichia coli DNA fragments by enzyme-amplified coupling of streptavidin-peroxidase to immobilized biotinylated lacZ gene target sequences.…”

Section: Disposable Devices For Dna Detectionmentioning

confidence: 99%

Disposable Biosensors for Clinical Diagnosis

Janegitz¹,

Cancino²,

Zucolotto³

2014

j. nanosci. nanotech.

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We present an overview on the use of disposable electrochemical biosensors for diagnostics, focusing on the applications of these devices as immunosensors and DNA sensors in the point-of-care diagnostics. Analytical biosensors have emerged as efficient alternatives for the detection of innumerous diseases, because of their high specificity and the convenience of detecting the electrochemical signals produced by the presence of an analyte using a portable equipment. This review highlights the recently developed strategies toward immobilization of different biological molecules on disposable electrodes such as carbon nanotubes and metal nanoparticles. In the course of the review, we first introduced the disposable biosensors, followed by an overview of the immunosensors, and discussed the applications of DNA sensors and disposable biosensors in point-of-care diagnostics. We also have evaluated the prospects and future applications of these devices in the field of biomedical research.

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“…been used in several works, such as for bacteria [3][4][5][6][7]12], toxins [56,58] and pesticides [26,[29][30][31][32]36,37] detections. One reported example is related to the modification of Escherichia coli single-stranded DNA binding (SSB) protein with gold nanoparticles for electrochemical detection of DNA hybridization [4].…”

Section: Topical Clustermentioning

confidence: 99%

“…Indeed, in the last few years nanostructured materials, such as noble metal nanoparticles, quantum dots, and magnetic nanoparticles, have been employed in a broad spectrum of highly innovative approaches for assays of metal ions, small molecules, proteins and nucleic acids biomarkers, bacteria and other species [3,4,7,9,12,14]. In addition to the large surface-to-volume ratio that favours miniaturization, nanoparticles possess unique optical, electronic and magnetic properties depending on their core materials [2].…”

Section: Introductionmentioning

confidence: 99%

Nanomaterials Based Electrochemical Sensing Applications for Safety and Security

Marín¹,

Merkoçi

2012

Electroanalysis

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Nanomaterials based sensing systems provide a new class of rapid and low cost detection alternatives with interest in the field of safety and security applications. In this review we report the recent trends in the use of various nanomaterials such as nanoparticles, carbon nanotubes, nanowires and graphene to detect different safety and security related analytes (i.e. microorganisms, toxins, pesticides and explosives). Nanomaterials are used either as modifiers of the electrochemical transducers or as labels with the objective to enhance the electrochemical signal, improve the stability and in general the performance of the detection systems including their cost-efficiency. Most of the developed systems are shown to bring excellent improvements while being used in the laboratory. Their application in real sample and during in-field monitoring still needs a long way while issues such as the reproducibility, stability, mass production capability of the designed devices etc. shouldnt still yet be resolved.

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Genosensor for detection of four pneumoniae bacteria using gold nanostructured screen-printed carbon electrodes as transducers

Cited by 48 publications

References 30 publications

Wireless Point-of-Care Platform With Screen-Printed Sensors for Biomarkers Detection

Wireless Point-of-Care Platform With Screen-Printed Sensors for Biomarkers Detection

Disposable Biosensors for Clinical Diagnosis

Nanomaterials Based Electrochemical Sensing Applications for Safety and Security

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