Genomodifier capacity assay: a non-cell test using dsDNA molecules to evaluate the genotoxic/genoprotective properties of chemical compounds

Abstract

We describe here an ultrasensitive and fast protocol called a GEMO assay (genomodifier capacity assay).

Toxicity of irrigating solutions and pharmacological associations used in pulpectomy of primary teeth

“…It is an ultrasensitive, simple, fast and accurate test (Cadoná et al . ). The two main solutions (1% and 2.5% NaOCl and 2% CHX) had genotoxic potential in both tests.…”

Induction of cytotoxicity, oxidative stress and genotoxicity by root filling pastes used in primary teeth

“…The higher dsDNA fluorescence measured by the GEMO was associated with lower index damage measured by the alkaline comet assay (Cadoná et al . ). In contrast, it has been reported that calcium hydroxide pastes caused no DNA fragmentation in human osteosarcoma cells (Huang et al .…”

“…GEMO was performed according to Cadoná et al . (). In black 96‐well plate, pure calf thymus DNA (dsDNA) was diluted in a TE buffer (10 mmol L −1 Tris‐HCl, 1 mmol L −1 EDTA, pH 7.5).…”

“…Extracts causing breaks in double‐stranded DNA molecules are identified by reduced fluorescence when compared to the control group, which contained only dsDNA (Cadoná et al . ). The test was performed in triplicate, and the averaged values were considered for analysis.…”

“…The ultrasensitive PicoGreen ® dye is a fluorescent reagent that allows the quantification of double‐stranded DNA molecules in solution (Cadoná et al . ).…”