Genomics, phylogeny and in silico analysis of mitochondrial glutathione S-transferase-kappa from the camel Camelus dromedarius

Ataya, Farid S.; Al‐Jafari, Abdulaziz A.; Daoud, Mohamed S.; Al-Hazzani, Amal A.; Shehata, Afaf I.; Saeed, Hesham; Fouad, Dalia

doi:10.1016/j.rvsc.2014.04.004

Cited by 14 publications

(7 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Camel G6PD is an important cytosolic enzyme involved in carbohydrate metabolism and generating NADPH that consumed in the lipogenesis process and in the elimination of reactive oxygen species (ROS) through different metabolic pathways that involved NADPH as a coenzyme. Hence, G6PD deficiency impairs the ability of some cells such as erythrocytes to form NADPH resulting in hemolysis and anemia [2,19]. In this study we reported overexpression of recombinant C. dromedarius G6PD in E.coli BL21 (DE3) pLysS and its subsequent purification and characterization.…”

Section: Introductionmentioning

confidence: 92%

“…The one-humped camel appears to be the best model of a species that thrives in this type of Arabian Desert environment. The camel developed unique adapted mechanisms that allow it to withstand harsh desert environment such as direct exposure to high temperatures that may result in oxidative stress and cellular damage [19,[23][24][25]. One of the most important housekeeping genes is the G6PD which plays a significant role in maintaining the levels of NADPH for fatty acid synthesis, generation of pentose sugars for nucleotide synthesis and elimination of the generated toxic ROS resulted from different metabolic pathways and environmental insults [4].…”

Section: Over Expression and Purification Of C Dromedarius G6pdmentioning

confidence: 99%

“…The Arabian camel are continuously exposed to toxic harmful agents from the environment and from different metabolic processes that produce reactive oxygen species (ROS). If not eliminated, ROS exerts damage to DNA and proteins and therefore pose a major threat to the genetic integrity of cells [19]. Camel G6PD is an important cytosolic enzyme involved in carbohydrate metabolism and generating NADPH that consumed in the lipogenesis process and in the elimination of reactive oxygen species (ROS) through different metabolic pathways that involved NADPH as a coenzyme.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Overexpression, purification and enzymatic characterization of a recombinant Arabian camel Camelus dromedarius glucose-6-phosphate dehydrogenase

Saeed

Ismaeil

Embaby

et al. 2018

Protein Expression and Purification

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 92%

Section: Over Expression and Purification Of C Dromedarius G6pdmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Overexpression, purification and enzymatic characterization of a recombinant Arabian camel Camelus dromedarius glucose-6-phosphate dehydrogenase

Saeed

Ismaeil

Embaby

et al. 2018

Protein Expression and Purification

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

The Arabian camel, Camelus dromedarius interferon epsilon: Functional expression, in vitro refolding, purification and cytotoxicity on breast cancer cell lines

et al. 2019

View full text Add to dashboard Cite

The current study highlights, for the first time, cloning, overexpression and purification of the novel interferon epsilon (IFNƐ), from the Arabian camel Camelus dromedaries. The study then assesses the cytotoxicity of IFNε against two human breast cancer cell lines MDA-MB-231 and MCF-7. Full-length cDNA encoding interferon epsilon (IFNε) was isolated and cloned from the liver of the Arabian camel, C . dromedarius using reverse transcription-polymerase chain reaction. The sequence analysis of the camel IFNε cDNA showed a 582-bp open reading frame encoding a protein of 193 amino acids with an estimated molecular weight of 21.230 kDa. A BLAST search analysis revealed that the C . dromedarius IFNε shared high sequence identity with the IFN genes of other species, such as Camelus ferus , Vicugna pacos , and Homo sapiens . Expression of the camel IFNε cDNA in Escherichia coli gave a fusion protein band of 24.97 kDa after induction with either isopropyl β-D-1-thiogalactopyranoside or lactose for 5 h. Recombinant IFNε protein was overexpressed in the form of inclusion bodies that were easily solubilized and refolded using SDS and KCl. The solubilized inclusion bodies were purified to apparent homogeneity using nickel affinity chromatography. We examined the effect of IFNε on two breast cancer cell lines MDA-MB-231 and MCF-7. In both cell lines, IFNε inhibited cell survival in a dose dependent manner as observed by MTT assay, morphological changes and apoptosis assay. Caspase-3 expression level was found to be increased in MDA-MB-231 treated cells as compared to untreated cells.

show abstract

“…This domesticated camel is one of the most important animals in the Arabian Peninsula, having high cultural and economic value. In Saudi Arabia, it comprises 16% of the animal biomass and is considered as the main source of meat [26, 27]. The aim of the present study was the isolation of full-length C. dromedarius IFNε gene, followed by its expression in Escherichia coli , in vitro refolding of the recombinant protein, purification, and characterization of the purified IFNε protein.…”

Section: Introductionmentioning

confidence: 99%

The Arabian camel,Camelus dromedariusinterferon epsilon: functional expression,in vitrorefolding, purification and cytotoxicity on breast cancer cell lines MDA-MB-231 and MCF-7

Abdel-Fattah

Saeed

El-Shennawy

et al. 2019

Preprint

View full text Add to dashboard Cite

18The current study highlights for the first time cloning, overexpression, purification, and assessing 19 the cytotxcity of the novel interferon epsilon (IFN ), from the Arabian camel Camelus 20 dromedarius, against two human breast cancer cell lines MDA-MB-231 and MCF-7. Full-length 21 cDNA encoding interferon epsilon (IFN) was isolated and cloned from the liver of the Arabian 22 camel, C. dromedarius using reverse transcription-polymerase chain reaction. The sequence 23 analysis of the camel IFN cDNA showed a 582-bp open reading frame encoding a protein of 193 24 amino acids with an estimated molecular weight of 22.953 kDa. A BLAST search analysis revealed 25 that the C. dromedarius IFN shared high sequence identity with the IFN genes of other species, 26 such as Camelus ferus, Vicugna pacos, and Homo sapiens. Expression of the camel IFN cDNA in 27 Escherichia coli gave a fusion protein band of 22.73 kDa after induction with either isopropyl β-D-28 1-thiogalactopyranoside or lactose for 5 h. Recombinant IFN protein was overexpressed in the 29 form of inclusion bodies that were easily solubilized and refolded using SDS and KCl. The 30 solubilized inclusion bodies were purified to apparent homogeneity using nickel affinity 31 chromatography. We examined the effect of IFN on two breast cancer cell lines MDA-MB-231 32 and MCF-7. In both cell lines, IFN inhibited cell survival in a dose dependent manner as observed 33 by MTT assay, morphological changes and apoptosis assay. Caspase-3 expression level was found 34 to be increased in MDA-MB-231 treated cells as compared to untreated cells.35 36 37 virally infected chick cells, and they called it interferon (IFN), a substance that interferes with 43 influenza and vaccinia virus replication [1]. Since then, the efforts to discover, characterize, and 44 develop new interferons as major therapeutic proteins have continued for over 60 years [2]. IFNs 45 are members of a large cytokine family of evolutionarily conserved pleiotropic regulators of cellular 46 functions; they are relatively low-molecular weight signaling proteins (20-25 kDa) usually 47 glycosylated and produced by a variety of cells, such as epithelia, endothelia, stroma, and cells of 48 the immune system [3-5]. The expression of IFNs is induced by a variety of different stimuli 49 associated with viral infections, bacteria, parasites, inflammation, and tumorigenesis [6]. IFNs, 50 therefore, induce a diverse range of biological functions and responses, including cell proliferation 51 and differentiation, inflammation, chemotaxis, immune cell (natural killer cells and macrophages) 52 activation, and apoptosis [7, 8]. The key to understanding these regulatory proteins lies in the 53 recognition of their pleiotropism, overlapping activities, functional redundancies, and side effects 54 [3]. Based on the type of receptors they interact with for signal transduction, IFNs are classified into 55 three major types namely, type I, II, and III, which have different gene and protein structures and 56 biological a...

show abstract

Genomics, phylogeny and in silico analysis of mitochondrial glutathione S-transferase-kappa from the camel Camelus dromedarius

Cited by 14 publications

References 53 publications

Overexpression, purification and enzymatic characterization of a recombinant Arabian camel Camelus dromedarius glucose-6-phosphate dehydrogenase

Overexpression, purification and enzymatic characterization of a recombinant Arabian camel Camelus dromedarius glucose-6-phosphate dehydrogenase

The Arabian camel, Camelus dromedarius interferon epsilon: Functional expression, in vitro refolding, purification and cytotoxicity on breast cancer cell lines

The Arabian camel,Camelus dromedariusinterferon epsilon: functional expression,in vitrorefolding, purification and cytotoxicity on breast cancer cell lines MDA-MB-231 and MCF-7

Contact Info

Product

Resources

About