Overexpression, purification and enzymatic characterization of a recombinant Arabian camel Camelus dromedarius glucose-6-phosphate dehydrogenase

Saeed, Hesham; Ismaeil, Mohammad; Embaby, Amira M.; Ataya, Farid S.; Malik, Ajamaluddin; Shalaby, Manal; El-Banna, Sabah G; Ali, Ahmed; Bassiouny, Khalid

doi:10.1016/j.pep.2015.09.002

Cited by 2 publications

(4 citation statements)

References 34 publications

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“…Numerous G6PDs isolated from mesophiles and thermophiles have been characterized [4,5,6,7,8,22,24,25,27,30,33], but only a few psychrophilic G6PD are known [9,10]. As a result, the functioning mechanism of psychrophilic G6PDs at low temperatures remains largely unraveled.…”

Section: Discussionmentioning

confidence: 99%

“…A similar mechanism is also observed in SpG6PD1, wherein the K m G6P values are markedly higher, whereas the thermal stability and activation enthalpy (Δ H ‡ ) are lower than those of mesophilic and thermophilic counterparts (Table 4 and Table 5). Moreover, compared to the cold-adapted and mesophilic counterparts that maintain their initial enzyme activity at moderate temperatures, and thermophilic G6PDs, which retain their activity for 2 h at 70 °C [6,7,10,24,26], the activity of SpG6PD1 was found to be dramatically decreased at moderate temperature (Figure 4). However, SpG6PD1 still retained high enzyme activity at 4 °C (Figure 3).…”

Section: Discussionmentioning

confidence: 99%

“…SpG6PD1 ( Sphingomonas sp. PAMC 26621), SeG6PD ( Sphingomonas elodea [20]), PfG6PD ( Pseudomonas fluorescens [21]), LmG6PD ( Leuconostoc mesenteroides [5]), HmG6PD (Human [22]), EcG6PD ( Escherichia coli [23]), CdG6PD ( Camelus dromedarius [24]), HvG6PD ( Hordeum vulgare [25]), AqG6PD ( Aquifex aeolicus VF5 [7]), TtG6PD ( Thermoanaerobacter tengcongensis [6]), TmG6PD ( Thermotoga maritima [26]). Catalytic residues Asp174, His175, and His237 (turquoise color), the substrate-binding motifs (yellow color), cofactor-binding pockets (green color).…”

Section: Figurementioning

confidence: 99%

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Cloning, Expression, and Characterization of a Psychrophilic Glucose 6-Phosphate Dehydrogenase from Sphingomonas sp. PAMC 26621

TranNgoc

Pham

Lee

et al. 2019

IJMS

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Glucose 6-phosphate dehydrogenase (G6PD) (EC 1.1.1.363) is a crucial regulatory enzyme in the oxidative pentose phosphate pathway that provides reductive potential in the form of NADPH, as well as carbon skeletons for the synthesis of macromolecules. In this study, we report the cloning, expression, and characterization of G6PD (SpG6PD1) from a lichen-associated psychrophilic bacterium Sphingomonas sp. PAMC 26621. SpG6PD1 was expressed in Escherichia coli as a soluble protein, having optimum activity at pH 7.5–8.5 and 30 °C for NADP+ and 20 °C for NAD+. SpG6PD1 utilized both NADP+ and NAD+, with the preferential utilization of NADP+. A high Km value for glucose 6-phosphate and low activation enthalpy (ΔH‡) compared with the values of mesophilic counterparts indicate the psychrophilic nature of SpG6PD1. Despite the secondary structure of SpG6PD1 being maintained between 4–40 °C, its activity and tertiary structure were better preserved between 4–20 °C. The results of this study indicate that the SpG6PD1 that has a flexible structure is most suited to a psychrophilic bacterium that is adapted to a permanently cold habitat.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Cloning, Expression, and Characterization of a Psychrophilic Glucose 6-Phosphate Dehydrogenase from Sphingomonas sp. PAMC 26621

TranNgoc

Pham

Lee

et al. 2019

IJMS

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“…G6PD catalyses the conversion of glucose 6-phosphate to 6phosphoglucono-d-lactone with reduction of NADP + to NADPH and NADPH serves as hydrogen and electron donor for a variety of reductive reactions. G6PD deficiency causes worse inflammatory response during disease progression in IBDs and is found to be new promising target for cancer therapy as it is involved in apoptosis and angiogenesis [24][25][26] . b-Glucosidases play key roles in a variety of essential physiological processes as the b-glucosidase activity is important representative indices for the activity of probiotics related to IBDs [27][28][29][30] .…”

Section: Introductionmentioning

confidence: 99%

In vivoeffects of curcumin on the paraoxonase, carbonic anhydrase, glucose-6-phosphate dehydrogenase andβ-glucosidase enzyme activities in dextran sulphate sodium-induced ulcerative colitis mice

Yıldırım

Sunay

Sinan

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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Increases in the risk of infections and malignancy due to immune suppressive therapies of inflammatory bowel diseases (IBDs) have led the researchers to focus on more nontoxic and acceptable natural products like curcumin. Here we investigate whether prophylactic and therapeutic application of the curcumin alters the enzyme activities of paraoxonase (PON), carbonic anhydrase (CA), glucose-6-phosphate dehydrogenase (G6PD) and cytosolic β-glucosidase in dextran sulphate sodium (DSS)-induced ulcerative colitis mice. Prophylactic application of curcumin resulted in higher MPO activity, less body weight loss and longer colon lengths compared to therapeutic group indicating preventive role of curcumin in IBDs. DSS-induced decrease in liver and serum PON activities were completely recovered by prophylactic administration of curcumin. DSS-induced reduction in liver cytosolic β-glucosidase activity was not affected by curcumin neither in the prophylactic group nor in the therapeutic group. Erythrocyte CA activity was significantly increased in curcumin groups, however no remarkable change in G6PD activity was observed.

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Overexpression, purification and enzymatic characterization of a recombinant Arabian camel Camelus dromedarius glucose-6-phosphate dehydrogenase

Cited by 2 publications

References 34 publications

Cloning, Expression, and Characterization of a Psychrophilic Glucose 6-Phosphate Dehydrogenase from Sphingomonas sp. PAMC 26621

Cloning, Expression, and Characterization of a Psychrophilic Glucose 6-Phosphate Dehydrogenase from Sphingomonas sp. PAMC 26621

In vivoeffects of curcumin on the paraoxonase, carbonic anhydrase, glucose-6-phosphate dehydrogenase andβ-glucosidase enzyme activities in dextran sulphate sodium-induced ulcerative colitis mice

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