Genomics of Viral–Soybean Interactions

Maroof, M. A. Saghai; Tucker, David M.; Tolin, S. A.

doi:10.1007/978-0-387-72299-3_16

Cited by 17 publications

(6 citation statements)

References 119 publications

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“…These isogenic lines provide unique genetic materials that could facilitate global gene expression studies with the aid of microarray technology in future experiments. Isogenic lines that possess one, two, or all three of the identified SMV resistance genes, in the same susceptible background, should help our understanding of the epistatic interactions and genetic mechanisms between these distinct loci from different sources (Saghai Maroof et al, 2008). Identifying additional genes or modifiers for SMV resistance will be necessary to create a durable, broad‐spectrum resistance in future cultivars.…”

Section: Discussionmentioning

confidence: 99%

Pyramiding of Soybean Mosaic Virus Resistance Genes by Marker‐Assisted Selection

Maroof

Jeong

Gunduz³

et al. 2008

Crop Science

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Soybean mosaic virus (SMV) causes a disease of soybean [Glycine max (L.) Merr.] that is prevalent throughout the United States. The disease can be effectively managed through the deployment of single‐dominant resistance genes known as Rsv genes that confer resistance to different strains of SMV. Pyramiding respective Rsv genes from different loci (Rsv1, Rsv3, and Rsv4) through marker‐assisted selection (MAS) is an ideal method for creating durable and wide spectrum resistance to all strains of SMV. In this study, simple sequence repeat markers were used to create isogenic lines of the susceptible cultivar Essex containing one, two, or three Rsv loci for observing background and epistatic effects of Rsv1, Rsv3, and Rsv4 on inoculation with six strains of SMV. Results indicate that an Essex background or modifier genes from the donor source had effects on reactions of Rsv3 and Rsv4 genes, causing the isogenic lines to be more susceptible than the Rsv donor parents. Two‐gene and three‐gene isolines of Rsv1Rsv3, Rsv1Rsv4 and Rsv1Rsv3Rsv4, acted in a complementary manner, conferring resistance against all strains of SMV, whereas isolines of Rsv3Rsv4 displayed a late susceptible reaction to selected SMV strains. We demonstrate with MAS and three near‐isogenic lines, each containing a different SMV‐resistance gene, that pyramided lines can be generated in a straightforward manner into two‐ or three‐gene–containing lines with high levels of resistance to SMV.

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Section: Discussionmentioning

confidence: 99%

Pyramiding of Soybean Mosaic Virus Resistance Genes by Marker‐Assisted Selection

Maroof

Jeong

Gunduz³

et al. 2008

Crop Science

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“…More than 100 viruses are known to infect soybean, and of these, at least 46 have been detected in naturally occurring infections in fields [ 6 , 9 ]. Some of these viruses, such as soybean mosaic virus (SMV) are globally distributed and threaten soybean production in many countries [ 10 – 12 ]. In contrast, bean pod mottle virus (BPMV) is recognized as a major soybean pathogen mainly in the United States of America (USA) [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Detection and discovery of plant viruses in soybean by metagenomic sequencing

Elmore

Groves

Hajimorad

et al. 2022

Virol J

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Background Viruses negatively impact soybean production by causing diseases that affect yield and seed quality. Newly emerging or re-emerging viruses can also threaten soybean production because current control measures may not be effective against them. Furthermore, detection and characterization of new plant viruses requires major efforts when no sequence or antibody-based resources are available. Methods In this study, soybean fields were scouted for virus-like disease symptoms during the 2016–2019 growing seasons. Total RNA was extracted from symptomatic soybean parts, cDNA libraries were prepared, and RNA sequencing was performed using high-throughput sequencing (HTS). A custom bioinformatic workflow was used to identify and assemble known and unknown virus genomes. Results Several viruses were identified in single or mixed infections. Full- or nearly full-length genomes were generated for tobacco streak virus (TSV), alfalfa mosaic virus (AMV), tobacco ringspot virus (TRSV), soybean dwarf virus (SbDV), bean pod mottle virus (BPMV), soybean vein necrosis virus (SVNV), clover yellow vein virus (ClYVV), and a novel virus named soybean ilarvirus 1 (SIlV1). Two distinct ClYVV isolates were recovered, and their biological properties were investigated in Nicotiana benthamiana, broad bean, and soybean. In addition to infections by individual viruses, we also found that mixed viral infections in various combinations were quite common. Conclusions Taken together, the results of this study showed that HTS-based technology is a valuable diagnostic tool for the identification of several viruses in field-grown soybean and can provide rapid information about expected viruses as well as viruses that were previously not detected in soybean.

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“…In the United States, Cho and Goodman used 8 soybean cultivars to classify 98 SMV isolates into seven strains, namely, G1 to G7 (Cho and Goodman 1979, 1982). Four dominant genes, Rsv1 , Rsv3 , Rsv4, and Rsv5, confer resistance to the US SMV strains have been mapped to three chromosomes 2,13, and 14, respectively (Klepadlo et al ., 2017; Saghai Maroof et al ., 2008).…”

Section: Introductionmentioning

confidence: 99%

Rsc3K, a SMV resistance gene localized on chromosome 2 in soybean cv. Kefeng No.1, interacts with virulence determinant P3

Jin

Yin

Xue

et al. 2022

Preprint

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Soybean mosaic virus (SMV) is one of the most devastating viral pathogens in Glycine max (L.) Merr (soybean). Twenty-two SMV strains (SC1-SC22) isolated in China were identified based on their responses to ten soybean cultivars. By using the F2-derived F3 (F2:3) and recombinant inbred line (RIL) populations of resistant Soybean cultivar (cv.) Kefeng No.1 × susceptible cv. Nannong 1138-2, we localized the gene mediating resistant to SMV-SC3 strain to a 90 kb interval on chromosome 2 in Kefeng No.1. Bean pod mottle vi-rus (BPMV)-induced gene silencing (VIGS) were used to study the gene function of candidate genes in the mapping interval and revealed that an recombinant gene, later named as Rsc3K, caused by internal deletion of a genomic DNA fragement in Kefeng No.1, is the resistant gene to SMV-SC3. By shuffling genes between avirulent isolate SC3 and avirulent SMV isolate 1129, we found that P3 is the virulence determinant causing resistance on Kefeng No.1. We showed the interaction between Rsc3K and P3 by the yeast two-hybrid (Y2H) and bimolecular fluorescent complementation (BiFC) assays. In conclusion, this study demonstrated that Rsc3K plays a crucial role in resistance of Kefeng No.1 to SMV-SC3 by direct interaction with viral protein P3.

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Genomics of Viral–Soybean Interactions

Cited by 17 publications

References 119 publications

Pyramiding of Soybean Mosaic Virus Resistance Genes by Marker‐Assisted Selection

Pyramiding of Soybean Mosaic Virus Resistance Genes by Marker‐Assisted Selection

Detection and discovery of plant viruses in soybean by metagenomic sequencing

Rsc3K, a SMV resistance gene localized on chromosome 2 in soybean cv. Kefeng No.1, interacts with virulence determinant P3

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