Pyramiding of Soybean Mosaic Virus Resistance Genes by Marker‐Assisted Selection

Abstract

Soybean mosaic virus (SMV) causes a disease of soybean [Glycine max (L.) Merr.] that is prevalent throughout the United States. The disease can be effectively managed through the deployment of single‐dominant resistance genes known as Rsv genes that confer resistance to different strains of SMV. Pyramiding respective Rsv genes from different loci (Rsv1, Rsv3, and Rsv4) through marker‐assisted selection (MAS) is an ideal method for creating durable and wide spectrum resistance to all strains of SMV. In this study, simple sequence repeat markers were used to create isogenic lines of the susceptible cultivar Essex containing one, two, or three Rsv loci for observing background and epistatic effects of Rsv1, Rsv3, and Rsv4 on inoculation with six strains of SMV. Results indicate that an Essex background or modifier genes from the donor source had effects on reactions of Rsv3 and Rsv4 genes, causing the isogenic lines to be more susceptible than the Rsv donor parents. Two‐gene and three‐gene isolines of Rsv1Rsv3, Rsv1Rsv4 and Rsv1Rsv3Rsv4, acted in a complementary manner, conferring resistance against all strains of SMV, whereas isolines of Rsv3Rsv4 displayed a late susceptible reaction to selected SMV strains. We demonstrate with MAS and three near‐isogenic lines, each containing a different SMV‐resistance gene, that pyramided lines can be generated in a straightforward manner into two‐ or three‐gene–containing lines with high levels of resistance to SMV.

Marker-assisted identification of resistance genes to soybean mosaic virus in soybean lines

“…The results are consistent with previous reports that many SMV-resistant cultivars contain two or more SMV resistance gene(s) (Ma et al 1995Chen et al 2001;Zheng et al 2006). Thus, our results suggest that future SMV breeding efforts should be directed toward pyramiding SMV resistance genes into elite soybean lines, as demonstrated in a recent report by Saghai Maroof et al (2008). Our analysis, performed by combining inheritance studies and molecular marker analysis, determined the type and number of SMV resistance genes in several soybean cultivars.…”

Resistance to Asian soybean rust in soybean lines with the pyramided three Rpp genes

“…In soybean, the pyramiding of resistance genes is known for its high and broad-spectrum resistance to soybean mosaic virus (Saghai Maroof et al 2008). The pyramiding of available ASR resistance genes in a single soybean cultivar may also provide more durable resistance against P. pachyrhizi populations expressing a wide range of pathogenicity in the field .…”

Magnaporthe oryzae Genetic Diversity and Its Outcomes on the Search for Durable Resistance

“…MAS of hypostatic genes would facilitate their maintenance in pyramiding populations. Molecular markers tightly linked to resistance genes have facilitated their pyramiding into single elite cultivars, with the possibility of creating more durable or broad spectrum resistance (Saghai-Maroof et al, 2008).…”