Genomic Sequence Analysis of the Multidrug-Resistance Region of Avian Salmonella enterica serovar Indiana Strain MHYL

Abstract

A series of human and animal diseases that are caused by Salmonella infections pose a serious threat to human health and huge economic losses to the livestock industry. We found antibiotic resistance (AR) genes in the genome of 133 strains of S. Indiana from a poultry production site in Shandong Province, China. Salmonella enterica subsp. enterica serovar Indiana strain MHYL had multidrug-resistance (MDR) genes on its genome. Southern blot analysis was used to locate genes on the genomic DNA. High-throughput sequencing technology was used to determine the gene sequence of the MHYL genome. Areas containing MDR genes were mapped based on the results of gene annotation. The AR genes blaTEM, strA, tetA, and aac(6′)-Ib-cr were found on the MHYL genome. The resistance genes were located in two separate MDR regions, RR1 and RR2, containing type I integrons, and Tn7 transposons and multiple IS26 complex transposons with transposable functions. Portions of the MDR regions were determined to be highly homologous to the structure of plasmid pAKU_1 in S. enterica serovar Paratyphi A (accession number: AM412236), SGI11 in S. enterica serovar Typhimurium (accession number: KM023773), and plasmid pS414 in S. Indiana (accession No.: KC237285).

Prevalence of Salmonella Isolates and Their Distribution Based on Whole-Genome Sequence in a Chicken Slaughterhouse in Jiangsu, China

“…By correlating the core genome to the genotypes of antibiotic resistance genes, we observed a high diversity of the antibiotic resistance genes in the predominant cluster of S. Kentucky isolates (Figure 2 and Table S6), indicating that the multidrug resistance of S. Kentucky was less related to the core genome. Previous studies showed that S. Kentucky were multidrug-resistance serotypes (38)(39)(40)(41), while S. Kentucky isolates in this study contained antibiotic resistance gene from more than five different antibiotic groups. S. Enteritidis isolates in this study showed a close relationship of the core genome clusters to the genotypes of its antibiotic resistance genes (Figure 3 and Table S7).…”

Isolation and characterization of Salmonella paratyphi phage and its lytic spectrum that correlation with pili

“…It's regrettable of that overuse of antibiotics has increased the severity of salmonella [29]. The main reasons of drug resistance was that antibiotic resistance (AR) and multidrug-resistance (MDR) genes were in the genome of almost all Salmonella strains [30].…”

Comparison of Phenotype and Genotype Virulence and Antimicrobial Factors of Salmonella Typhimurium Isolated from Human Milk

“…The following supporting information can be downloaded at: . References [ 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 ] are cited in the supplementary materials.…”