Genomic organization of Borna disease virus.

Briese, Thomas; Schneemann, Anette; Lewis, Ann; Park, Yoo-Sun; Kim, S.; Ludwig, Hanns; Lipkin, W. Ian

doi:10.1073/pnas.91.10.4362

Cited by 282 publications

(341 citation statements)

References 28 publications

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“…Borna disease virus (BDV) is a noncytolytic, neurotropic, single strand, negative sense RNA virus that naturally infects a wide range of vertebrate species from birds and rodents to primates [1,3]. BDV can also cause encephalomyelitis in a wide range of experimental animals [5,6,14].In domestic cats, an characteristic disease of the central nervous system has been observed in Sweden [9]; the dominating clinical sign is an unsteady (staggering) gait. Histopathological examination of the cats showed that the inflammatory reaction of the central nervous system was most pronounced in the grey matter of the brain stem, basal ganglia and hippocampus.…”

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Detection of Anti-Borna Disease Virus Antibodies from Cats in Asian Countries, Japan, Philippines and Indonesia Using Electrochemiluminescence Immunoassay.

Horii¹,

Garcia

Noviana

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. Anti-Borna disease virus (BDV) antibodies were detected from cats in Japan, Philippines and Indonesia by using electrochemiluminescence immunoassay. Positive rates were 3.1%, 3.8% and 2.0% in Japan, Philippines and Indonesia, respectively. There was no differences in the positive rate of anti-BDV antibodies between male and female cats and among habitats. While, a significantly (P<0.05) higher prevalence (6.5%) was found in the oldest age group (more than 6 years) cats. KEY WORDS: Borna disease virus (BDV), electrochemiluminescence immunoassay (ECLIA), feline.J. Vet. Med. Sci. 63(8): 921-923, 2001 Borna disease is an infectious neurological disease that occurs sporadically in horses and sheep in Central Europe. Borna disease virus (BDV) is a noncytolytic, neurotropic, single strand, negative sense RNA virus that naturally infects a wide range of vertebrate species from birds and rodents to primates [1,3]. BDV can also cause encephalomyelitis in a wide range of experimental animals [5,6,14].In domestic cats, an characteristic disease of the central nervous system has been observed in Sweden [9]; the dominating clinical sign is an unsteady (staggering) gait. Histopathological examination of the cats showed that the inflammatory reaction of the central nervous system was most pronounced in the grey matter of the brain stem, basal ganglia and hippocampus. These changes and laboratory findings including leukopenia and elevations in protein content and white blood cell count of the cerebrospinal fluid in affected cats suggested that staggering disease is infectious and most probably caused by virus. By using an indirect immunofluorescence assay (IFA), anti-BDV antibody has been detected in the cats with typical staggering disease [10,13]. Recently, the high prevalence of BDV infections has been demonstrated in domestic cats with neurological disorders in Japan [11].A seroepidemiological survey of the cat has been done by using IFA and/or Western blot (WB) analysis also in Japan [12]. Since the antibody titers were usually very low in Borna disease, an establishment of sensitive and reliable method to detect a small amount of anti-BDV antibodies is required. Recently, we have developed a new system, electrochemiluminescence immunoassay (ECLIA), using recombinant BDV p24 and p40 proteins, which has the sensitivity and specificity to serve as a serological screening method for measuring anti-BDV antibodies [16]. In this study we tried to detect anti-BDV antibodies of domestic cats in Asian countries and to compare the prevalence of BDV among the countries.Blood samples were collected from domestic cats at veterinary hospitals of University of Philippines Los Baños, Laguna, Philippines; Faculty of Veterinary Science, Bogor Agricultural University, Bogor, Indonesia; Osaka, Kitakyushu, Kumamoto and Nagasaki, Japan. Serum samples were stored at -30°C until use.To measure antibody against BDV, ECLIA is performed as previously described [16]. Briefly, micro beads were coated with horse-derived BDV p24 and p40 reco...

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Detection of Anti-Borna Disease Virus Antibodies from Cats in Asian Countries, Japan, Philippines and Indonesia Using Electrochemiluminescence Immunoassay.

Horii¹,

Garcia

Noviana

et al. 2001

The Journal of Veterinary Medical Science

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“…Two isolates (reference strains V and He/80) were recently completely sequenced. BDV was classified into the Mononegavirales order and it is the only prototype of the new Bornaviridae family within this order [9,15].…”

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confidence: 99%

Borna disease: current knowledge and virus detection in France

Dauphin¹,

Legay

Pitel

et al. 2002

Vet. Res.

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-For over two centuries, Borna disease (BD) has been described as a sporadically occurring infectious meningoencephalomyelitis affecting horses and sheep in Central Europe. Over the last decade, the BD epidemiology has been discussed. Firstly, its geographical distribution seems larger than what was previously thought. Secondly, the disease can affect a large number of warm-blooded animal species, including humans. The aetiological agent is the Borna disease virus (BDV), an enveloped, nonsegmented negative-stranded RNA virus classified in the new virus family Bornaviridae (Mononegavirales order). It can induce severe clinical signs of encephalitis with striking behavioural disturbances and may cause death. BDV genome has recently been detected in France in the blood and brain of several animal species (horses, bovines, foxes). meningoencephalomyelitis / Borna disease virus / epidemiology / diagnosisRésumé -La maladie de Borna : connaissances actuelles et détection du virus en France. La maladie de Borna est connue depuis plus de deux siècles en Europe centrale comme une méningoencé-phalomyélite d'origine infectieuse affectant les chevaux et les moutons. Depuis ces dix dernières années, l'épidémiologie de la maladie a été révisée puisque la répartition géographique de la maladie de Borna s'avère plus large que rapportée jusqu'alors. En outre, elle peut affecter un grand nombre d'espèces animales à sang chaud, y compris l'homme. L'agent étiologique est le BDV (virus de la maladie de Borna), virus enveloppé, à ARN simple brin, non segmenté et de polarité négative, récem-ment classifié dans la nouvelle famille des Bornaviridae, (ordre des Mononegavirales). Le BDV peut induire des signes cliniques sévères d'encéphalite virale avec des troubles comportementaux importants et il peut entraîner la mort de l'animal. Récemment, le génome du BDV a été mis en évidence en France dans le sang et le cerveau de plusieurs espèces animales (chevaux, bovins, renards). méningoencéphalomyélite / virus de la maladie de Borna / épidémiologie / diagnostic 127

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“…virus Borna / PCR / témoin interne / tissu biologique partial segment 2 of serotype 3 sequence and the primer recognition sites of two BDV targets: the p40 genomic region coding the nucleoprotein, and the p24 genomic region coding the phosphoprotein known as a cofactor of the BDV polymerase [4]. Since it contains another genomic fragment between the primer sites, this molecule cannot form a heteroduplex with the test template.…”

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Construction of an internal standard used in RT nested PCR for Borna Disease Virus RNA detection in biological samples

et al. 2000

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-The highly neurotropic Borna Disease Virus (BDV), which belongs to the Mononegavirales order -Bornaviridae family -is generally detected using the RT-nested-PCR. If false positive results (often caused by laboratory contaminations) can be avoided, some false negative results which are mostly due to inhibitory effects of some reaction components and/or to sample preparation errors, can occur. Thus, in order to control the RT-PCR sample, an RNA internal standard molecule named "mimic" was constructed with the same primer recognition sites as the viral nucleic acids, flanking a heterologous DNA fragment of distinct molecular weight. Because of their different sizes, the mimic and viral PCR products can be easily discriminated by agarose gel electrophoresis. The co-amplification of both BDV and mimic RNA was performed on infected cells and on biological tissues such as the brain and blood, commonly known to contain PCR inhibitor components. After mimic sensitivity studies were achieved (2.5 fg of "p40 RNA mimic" and 0.25 fg of "p24 RNA mimic"), the competitive amplification reaction between both BDV and mimic RNA was performed on these tissues. The results confirmed that nervous tissue has an inhibitory effect on RT-PCR, which supports the necessity of BDV detection by a higher sensitive method such as RT nested PCR. Moreover, these results confirmed the interest of an internal standard for BDV RNA detection in biological samples. Borna virus / PCR / internal standard / biological tissueRésumé -Construction d'un témoin interne de RT-PCR nichée pour la détection du génome du virus de la maladie de Borna dans des tissus biologiques. Le génome du virus de la maladie de Borna (« Borna disease Virus » -BDV), du fait des propriétés biologiques de ce virus, n'est généralement détectable que par la méthode RT-PCR nichée. Si les résultats « faux-positifs » peuvent être évités par des conditions de manipulation très strictes, les résultats « faux-négatifs » demeurent bien Vet. Res. 31 (2000) 565-572 565

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Genomic organization of Borna disease virus.

Cited by 282 publications

References 28 publications

Detection of Anti-Borna Disease Virus Antibodies from Cats in Asian Countries, Japan, Philippines and Indonesia Using Electrochemiluminescence Immunoassay.

Detection of Anti-Borna Disease Virus Antibodies from Cats in Asian Countries, Japan, Philippines and Indonesia Using Electrochemiluminescence Immunoassay.

Borna disease: current knowledge and virus detection in France

Construction of an internal standard used in RT nested PCR for Borna Disease Virus RNA detection in biological samples

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