Genomic Organization and Sequence of the Mud Loach (Misgurnus mizolepis) Growth Hormone Gene: A Comparative Analysis of Teleost Growth Hormone Genes

Noh, Jae Koo; Cho, Kyou Nam; Nam, Yoon Kwon; Kim, Dong Soo; Kim, Chul Geun

doi:10.1016/s1016-8478(23)13596-5

Molecules and Cells

1999

DOI: 10.1016/s1016-8478(23)13596-5

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Genomic Organization and Sequence of the Mud Loach (Misgurnus mizolepis) Growth Hormone Gene: A Comparative Analysis of Teleost Growth Hormone Genes

Jae Koo Noh,

Kyou Nam Cho,

Yoon Kwon Nam

et al.

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Cited by 11 publications

References 22 publications

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Cloning, sequencing and expression of cDNA encoding growth hormone from Indian catfish (Heteropneustes fossilis)

et al. 2001

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A tissue-specific cDNA library was constructed using polyA+ RNA from pituitary glands of the Indian catfish Heteropneustes fossilis (Bloch) and a cDNA clone encoding growth hormone (GH) was isolated. Using polymerase chain reaction (PCR) primers representing the conserved regions of fish GH sequences the 3' region of catfish GH cDNA (540 bp) was cloned by random amplification of cDNA ends and the clone was used as a probe to isolate recombinant phages carrying the full-length cDNA sequence. The full-length cDNA clone is 1132 bp in length, coding for an open reading frame (ORF) of 603 bp; the reading frame encodes a putative polypeptide of 200 amino acids including the signal sequence of 22 amino acids. The 5' and 3' untranslated regions of the cDNA are 58 bp and 456 bp long, respectively. The predicted amino acid sequence of H. fossils GH shared 98% homology with other catfishes. Mature GH protein was efficiently expressed in bacterial and zebrafish systems using appropriate expression vectors. The successful expression of the cloned GH cDNA of catfish confirms the functional viability of the clone.

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Cloning, sequencing and expression of cDNA encoding growth hormone from Indian catfish (Heteropneustes fossilis)

et al. 2001

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Polymorphisms of the growth hormone gene and their association with growth traits and sex in Sarcocheilichthys sinensis

et al. 2020

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A comparative account of the structure of the growth hormone encoding gene and genetic interrelationship in six species of the genus Labeo

Ramasamy

Majumdar

2007

Fish Physiol Biochem

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The growth hormone (GH) gene isolated and cloned from various Labeo species (L. rohita, L. calbasu, L. fimbriatus, L. gonius, L. bata, and L. kontius) is shown to contain a single copy in the haploid genome, with an overall size of $2.5 kb. The GH gene in all the Labeo species studied has five exons and four introns of various sizes with the exon/ intron boundary sequence of GT/AG. The length variation of the GH gene between the species is found to be due to length variation in the form of several deletions in the third intron. The length of individual exons is the same in all the species with an open reading frame (ORF) of 630 bp (210 amino acids) except in L. rohita, which has a 9 bp deletion in the fourth exon, resulting in a shorter GH of 621 bp (207 amino acids). The similarity in the nucleotide and amino acid sequences between the different Labeo species is greater than 97%, in spite of eight amino acids being altered in the GH protein of Labeo that reside outside the conserved domain sequence required for its function. Nucleotide substitutions are seen in the form of 20 transitions and three transversions in the ORF of the GH gene.

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Genomic Organization and Sequence of the Mud Loach (Misgurnus mizolepis) Growth Hormone Gene: A Comparative Analysis of Teleost Growth Hormone Genes

Cited by 11 publications

References 22 publications

Cloning, sequencing and expression of cDNA encoding growth hormone from Indian catfish (Heteropneustes fossilis)

Cloning, sequencing and expression of cDNA encoding growth hormone from Indian catfish (Heteropneustes fossilis)

Polymorphisms of the growth hormone gene and their association with growth traits and sex in Sarcocheilichthys sinensis

A comparative account of the structure of the growth hormone encoding gene and genetic interrelationship in six species of the genus Labeo

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