Genomic Islands in Sinorhizobium meliloti Rm1021, Nitrogen-Fixing Symbiont of Alfalfa

The potency of bacteria isolated from the hydroponic rockwool of field mustard (Brassica rapa L.) for nitrogen fixation and indole acetic acid (IAA) production

“…The pH level and temperature of the water can be optimally conditioned for the growth and survival of microorganisms. Although some bacteria, such as Agrobacterium tumefaciens, Xylella fastidiosa, and Pseudomonas syringae can be pathogenic to plants, most bacteria in the hydroponic system are beneficial to plants (Roumiantseva et al, 2018). Many of these bacteria are able to suppress the growth of particular plant pathogenic bacteria to protect plants (Triwidodo, 2021).…”

Draft Genome Sequence of Sinorhizobium meliloti AK555

“…The genome of AK555 comprises a chromosome (SMc, circle of 3,675,317 bp), two circular megaplasmids (SMa, 1,658,858 bp, and SMb, 1,328,877 bp), and two cryptic plasmids (SMd, circle of 31,192 bp, and SMe, assembled from 3 contigs [440,009, 790, and 787 bp]). Genomic islands (GIs) as phage-related sequences with low G+C contents similar to those in strain Rm1021 (10) were not detected in the chromosome of AK555. Potential GIs represented by 41-kbp (G+C content, 58.67%; 91 open reading frames [ORFs]) and 51.4-kbp (G+C content, 59.7%; 108 ORFs) sequences integrated in tRNA-Gln (CTG) and in tRNA-Lys (CTT) with phage-related integrases and LigD (2 and 1, respectively) genes nearby.…”

Draft Genome Sequence of Sinorhizobium meliloti Strain AK170

“…The oriC sequence of AK170 (477 bp) has 100% identity with the corresponding sequences in genomes of Rm1021 (GenBank accession number AL591688) and AK83 (GenBank accession numbers NC_015590 and CP002781). The genome of AK170 does not contain any sequences similar to those which were identified as phage-related genomic islands in Rm1021 (10). Two of the three known open reading frames (ORFs) of hsd genes encoding a type I restriction-modification system and are known as a part of genomic island Sme19T of Rm1021 (10) were detected in genome of strain AK170.…”

“…The genome of AK170 does not contain any sequences similar to those which were identified as phage-related genomic islands in Rm1021 (10). Two of the three known open reading frames (ORFs) of hsd genes encoding a type I restriction-modification system and are known as a part of genomic island Sme19T of Rm1021 (10) were detected in genome of strain AK170. Both hsdR and hsdM of AK170 showed less than 70% nucleotide similarity with those in Rm1021 and more than 81% with the chromosome sequences of Rhizobium sp.…”