Mosquito Culex quinquefasciatus is a vector of nematode worms, namely Wuchereria bancrofti which is the cause of filariasis in tropical and subtropical countries. Distributed of Cx. quinquefasciatus is widely in Indonesia with differences the geographical, resulting in the adaptation to the environment and may results in a high variation, both phenotypic (morphology) and genotypic (genetic) variation. This study aims was to determine the genetic diversity of mosquitoes Cx.quinquefasciatus as vector filariasis in Pekalongan City and Regent. Genetic characterization performed by PCR-RAPD using three primers, ie OPA-11, OPA-12, and OPA-15. Data were analyzed by using UPGMA algorithm and Simple Matching Coefficient and presented as dendrogram. The results showed a high genetic diversity with the polymorphisms up to 100%. Keywords: Culex quinquefasciatus, vector, filariasis, PCR-RAPD PENDAHULUAN

Work that requires being in a humid area, can cause fungal infections. Skin fungal infections in humans can be divided into two, namely dermatophytosis and non-dermatophytosis. Dermatophytosis is a superficial fungal infection that attacks the keratinized parts of the skin, nails and hair and is caused by 3 genera, namely Microsporum, Epidermophyton, and Trichophyton. While non dermatophytosis is a fungal infection of the outer skin or this type of fungal infection does not reach the keratin tissue. Non-dermatophyte fungi include Aspergillus sp., Malessezia furfur. Due to dermatophyte and non-dermatophyte fungal infections, namely itching of the skin or earlobe. This activity aims to increase the understanding of cleaning workers at one of the universities in Surabaya about the dangers of fungus on the skin, so that they can prevent and anticipate early spread. The method used in this community service activity is the presentation and discussion of the dangers of skin fungal infections. To measure the level of understanding of the participants regarding the dangers of toxoplasmosis, a pretest was conducted at the beginning and at the end of the activity. This activity was attended by 22 janitors, both male and female. The average pretest score is 49,63 points, while the average posttest score is 71,48 points. These results indicate that there is an increase in the knowledge of cleaning workers at one of the universities in Surabaya about the causes, dangers, modes of transmission and methods of preventing skin fungal diseases. Educational activities are expected to be sustainable so that the community can apply steps to prevent skin fungal infections.

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Cockroaches are one of the most common types of insects around our neighborhood, and it is one of the most dangerous insects because some species of cockroaches are known to transmit disease in humans such as tuberculosis, typhoid, asthma, cholera, and hepatitis. Eradication of cockroaches can be done physically and chemically, the use of chemical-based insecticides can cause respiratory problems. In an insecticide drug containing natural ingredients contain essential oils, the content of essential oil compounds serve as an anti-insect drug on citronella. This study aimed to determine the effect of essential oils of citronella fragrance to cockroaches. This type of research was experimental. The population in this study were cockroaches (Periplaneta americana) obtained from the houses of villagers of Tanjung, Sampang, Madura. The total sample was 32 adult stage cockroaches. Data analysis was using free T test with error rate of 5% or 0,05. The results showed that cockroaches stopped moving with an average of 15.75. Based on T test results showed the significant results of 0.00 which was smaller than 0.05 then H0 was rejected Ha was accepted. It can be concluded that the essential oils of citronella fragrance have an effect on the death of cockroaches. Keywords:

Sugar disease or diabetes is commonly known in medical terms with diabetes mellitus. Diabetes mellitus is a disease characterized by an increase in blood glucose levels (hyperglycemia) that occurs due to the body's lack of the hormone insulin both absolute and relative.  Glucose is a form of carbohydrate metabolism that functions as the main source of energy controlled by insulin. Excess glucose is converted into glycogen which will be stored in the liver and muscles for backup if needed. Increased blood glucose levels occur in patients with Impaired Glucose Tolerance (TGT), Impaired Fasting Blood Sugar (GDPT) and Diabetes Mellitus.  Service activities in the form of education related to the dangers of Diabetes Mellitus, the use of natural ingredients as an alternative to lowering sugar levels and checking Random Blood Sugar levels.  There is an increase in public knowledge because  the post-test value is higher when compared to the pre-test value.   The  average pre-test score is 38.3 points while  the average post-test  score is 79.2 points.   And  the results of the GDA examination stated 23 people whose results were above normal and 13 people whose results were still within the normal range.