Genomic Insight and Optimization of Astaxanthin Production from a New Rhodotorula sp. CP72-2

Kingkaew, Engkarat; Tedsree, Nisachon; Phuengjayaem, Sukanya; Rojsitthisak, Pornchai; Sritularak, Boonchoo; Thitikornpong, Worathat; Thompho, Somphob; Mhuantong, Wuttichai; Tanasupawat, Somboon

doi:10.3390/fermentation9060501

Cited by 2 publications

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References 50 publications

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“…(teleomorph is Rhodosporidium) is one of the most studied strains. It is a promising source of pigments such as β-carotene, γcarotene, lycopene, torulene, and torularhodin [6][7][8]. Colonies can have an orange, salmon, pink, or red color depending on the type and concentration of pigments.…”

Section: Introductionmentioning

confidence: 99%

Current Advances in Carotenoid Production by Rhodotorula sp.

Ochoa-Viñals,

Alonso-Estrada,

Pacios-Michelena

et al. 2024

Fermentation

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Microbial carotenoids are pigments of lipophilic nature; they are considered promising substitutes for chemically synthesized carotenoids in the food industry. Their benefits for human health have been demonstrated due to their antioxidant capacity. Yeasts of the genus Rhodotorula have genotypic characteristics that allow them to accumulate high concentrations of carotenes under certain stress conditions. The present review includes recent information covering different aspects of carotenoid production in Rhodotorula sp. fermentation. This review focuses on fermentation carotenoid production strategies, describing various economic raw materials as sources of carbon and nitrogen, the capacity for tolerance to heavy metals, and the effect of light, pH, and salts on the accumulation of carotenoids. Genetic modification strategies used to obtain strains with increased carotenoid production are described. Furthermore, using magnetic nanoparticles in the fermentation system, which could be a stress factor that increases pigment production, is considered for the first time. Rhodotorula is a potential source of high-value carotenoids with applications in the cosmetics, pharmaceutical, and food industries.

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Section: Introductionmentioning

confidence: 99%

Current Advances in Carotenoid Production by Rhodotorula sp.

Ochoa-Viñals,

Alonso-Estrada,

Pacios-Michelena

et al. 2024

Fermentation

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Comparative genomic analysis and optimization of astaxanthin production of Rhodotorula paludigena TL35-5 and Rhodotorula sampaioana PL61-2

Hoondee,

Phuengjayaem,

Kingkaew

et al. 2024

PLoS ONE

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Astaxanthin is a powerful antioxidant known to enhance skin, cardiovascular, eye, and brain health. In this study, the genome insights and astaxanthin production of two newly isolated astaxanthin-producing yeasts (TL35-5 and PL61-2) were evaluated and compared. Based on their phenotypic and genotypic characteristics, TL35-5 and PL61-2 were identified as basidiomycetous yeasts belonging to Rhodotorula paludigena and Rhodotorula sampaioana, respectively. To optimize astaxanthin production, the effects of cultural medium composition and cultivation conditions were examined. The optimal conditions for astaxanthin production in R. paludigena TL35-5 involved cultivation in AP medium containing 10 g/L glucose as the sole carbon source, supplemented with 1.92 g/L potassium nitrate, pH 6.5, and incubation at 20°C for 3 days with shaking at 200 rpm. For R. sampaioana PL61-2, the optimal medium composition for astaxanthin production consisted of AP medium with 40 g/L glucose, supplemented with 0.67 g/L urea, pH 7.5, and the fermentation was carried out at 20°C for 3 days with agitating at 200 rpm. Under their optimal conditions, R. paludigena TL35-5 and R. sampaioana PL61-2 gave the highest astaxanthin yields of 3.689 ± 0.031 and 4.680 ± 0.019 mg/L, respectively. The genome of TL35-5 was 20,982,417 bp in length, with a GC content of 64.20%. A total of 6,789 protein-encoding genes were predicted. Similarly, the genome of PL61-2 was 21,374,169 bp long, with a GC content of 64.88%. It contained 6,802 predicted protein-encoding genes. Furthermore, all essential genes involved in astaxanthin biosynthesis, including CrtE, CrtYB, CrtI, CrtS, and CrtR, were identified in both R. paludigena TL35-5 and R. sampaioana PL61-2, providing evidence for their ability to produce astaxanthin.

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Genomic Insight and Optimization of Astaxanthin Production from a New Rhodotorula sp. CP72-2

Cited by 2 publications

References 50 publications

Current Advances in Carotenoid Production by Rhodotorula sp.

Current Advances in Carotenoid Production by Rhodotorula sp.

Comparative genomic analysis and optimization of astaxanthin production of Rhodotorula paludigena TL35-5 and Rhodotorula sampaioana PL61-2

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