Genomic heterogeneity in bladder cancer as detected by fluorescence <i>in situ</i> hybridization

Yokogi, Hiroyuki; Wada, Yukihiro; Moriyama-Gonda, Nobuko; Igawa, Mikio; Ishibe, Tomoyuki

doi:10.1046/j.1464-410x.1996.01786.x

Cited by 12 publications

(6 citation statements)

References 2 publications

(3 reference statements)

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“…Ozen [5] suggested that c-erbB2 and TP53 could be responsible for progression to high grade and lamina propria invasion. These studies and others [4,14,24] support the ®ndings of abnormalities in 7 and 17 reported here. However, there have been no studies associating such changes speci®cally with recurrence and it may be dangerous to extrapolate alterations associated with progression to a separate disease event.…”

Section: Diagnosis Of Recurrence In Tcc 45supporting

confidence: 91%

Aneusomy of chromosomes 7 and 17 predicts the recurrence of transitional cell carcinoma of the urinary bladder

Watters¹,

Ballantyne²,

Going

et al. 2000

BJU International

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Objective To determine if changes in chromosome 7 and 17 copy number can be used to predict recurrence in patients with primary noninvasive (pTa) or superficially invasive (pT1) transitional cell carcinoma (TCC) of the urinary bladder. Patients and methods Tissue specimens for 129 tumours from 52 patients (38 men and 14 women) with pTa/pT1 TCC at first diagnosis were retrieved from pathology archives. All patient notes were accessed and disease outcome documented for superficial (pTa/pT1) recurrence or progression to detrusor muscle invasion ( pT2). The tumours were examined for chromosomal copy number of chromosomes 7 and 17 using fluorescence in situ hybridization (FISH) with chromosome‐specific probes. The copy number of chromosomes 7 and 17 was determined in interphase nuclei on intact 6 µm tissue sections. Results Aneusomy of chromosomes 7 and 17 was detected in the index primary tumours of 10 of 32 (31%) patients with subsequent recurrent disease. No aneusomy for these chromosomes was detected in primary tumours from 20 patients with no detect‐able recurrence (P = 0.0082). The relative risk of recurrence was 3.62 times greater (95% confi‐dence interval 1.6–8.1, Cox’s multiple regression P = 0.0019) for patients with chromosomal aneusomy in primary TCC. Neither stage nor grade of the primary tumours was associated with recurrence in these patients, nor was there a significant association with increased grade (G2/3) or stage ( pT2) at recurrence. Conclusion These results suggest that the measurement of aneusomy by FISH, using markers for chromosomes 7 and 17, predict recurrence in a subgroup of patients with pTa/pT1 tumours at presentation. This finding may offer a new objective and quantitative test for patients destined to recur.

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Section: Diagnosis Of Recurrence In Tcc 45supporting

confidence: 91%

Aneusomy of chromosomes 7 and 17 predicts the recurrence of transitional cell carcinoma of the urinary bladder

Watters¹,

Ballantyne²,

Going

et al. 2000

BJU International

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“…FISH uses fluorescent-labelled DNA probes against the centromeres of specific chromosomes to demonstrate chromosomal deletion or duplication. Gene amplification can also be detected with FISH [10], FISH may provide important karyotypic information on individual cells during tumorigenesis [11,12]…”

Section: Fish -Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Diagnosis and Treatment of Superficial Transitional Cell Carcinoma of the Bladder: Facts and Perspectives

Kh¹

1997

Eur Urol

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“…Further investigations, especially in the follow-up of patients will clarify the value of FISH as a noninvasive, highly sensitive diagnostic tool for bladder cancer. [22] 26 +7, -9, -10, -11 Yokogi et al, 1996 [23] 30 +7, -9, +17…”

Section: Resultsmentioning

confidence: 99%

Fluorescence in situ Hybridization in the Diagnosis of Transitional Cell Carcinoma

Werner¹,

Ébert²,

Wunderlich³

et al. 1999

Oncol Res Treat

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Background: Cystoscopy is still considered to be the ‘Gold Standard’ in the diagnosis or urinary bladder cancer. However, this invasive technique is not free from subjective influences, especially in cases of superficial tumors of low malignancy. As is the case with cytological detection methods, cystoscopy suffers from a diagnostic gap in the early detection of urothelial carcinomas. Molecular genetic methods, on the other hand, are objective with regard to evaluation of early changes before any macroscopic tumor detection. Cytogenetic analyses of transitional cell carcinomas show structural changes of chromosomes 1, 3, 5, and 8 along with numerical aberrations of chromosomes 7, 8, 9, 10, and the Y chromosome. The detection of these changes in in vitro studies proved to be difficult for the need to establish tumor cell lines. Consequentially, fluorescence in situ hybridization (FISH) could be used as an alternative method to detect cytogenetic changes. Patients and Methods: Since 1995 we investigated 95 patients suffering from urinary bladder carcinoma in a prospective study. FISH was carried out on cells from spontaneous urine samples and bladder washes in parallel with conventional cytology. For chromosomes 7, 8, 9, and 12 centromere probes were used. Results: Comparing results on spontaneous urine samples and on bladder washes, the sensitivity of detecting tumor cells obtained by FISH was 27/32% (pTa), 72.6/92.8% (T1G1–2), and 73.3/86.6% (≤ pT1G3 and > pT1), respectively. In cases with papillomas and cystitis as well as in a control group, FISH yielded no false-positive results. Conclusion: Our data show that FISH is an objective, appropriate technique for diagnosis and follow-up of transitional cell carcinoma and represents a valuable complementation to the conventional cytological investigations.

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Genomic heterogeneity in bladder cancer as detected by fluorescence in situ hybridization

Cited by 12 publications

References 2 publications

Aneusomy of chromosomes 7 and 17 predicts the recurrence of transitional cell carcinoma of the urinary bladder

Aneusomy of chromosomes 7 and 17 predicts the recurrence of transitional cell carcinoma of the urinary bladder

Diagnosis and Treatment of Superficial Transitional Cell Carcinoma of the Bladder: Facts and Perspectives

Fluorescence in situ Hybridization in the Diagnosis of Transitional Cell Carcinoma

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