Genomic full‐length sequence of a novel HLA‐B*39:01:01:03 allele was identified in a Chinese individual

Abstract

HLA-B*39:01:01:03 allele differs from HLA-B*39:01:01:01 allele by a single nucleotide substitution.

A novel HLA‐B*15 allele, HLA‐B*15:326, was identified in a Chinese bone marrow donor

“…The genotype of the donor was HLA‐A*11:01,30:01 ; DRB1*07:01,12:01:01G ; while HLA‐B locus was assigned as HLA‐B*13:02,15:01 with one nucleotide mismatch at position 176 by the software. For further confirmation, we amplified the HLA‐B*13 and HLA‐B*15 alleles separately using allele‐specific primers and sequenced both alleles for exons 2–4 according to our previous reports . The amplified PCR products were sequenced using ABI PRISM BigDyeTM3.1 Terminator Kit (Applied Biosystems) according to the manufacturer's protocol.…”